GTPBP4 Antibody

Images

 
Western Blot: GTPBP4 Antibody [NBP2-16766] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7. 5% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: GTPBP4 Antibody [NBP2-16766] - A431 cells were fixed in iced-cold MeOH for 5 min. Green: GTPBP4 protein stained by GTPBP4 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Western Blot: GTPBP4 Antibody [NBP2-16766] - Sample (10 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:5000.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

GTPBP4 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human GTPBP4. The exact sequence is proprietary.
Localization
Nucleus; nucleolus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GTPBP4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GTPBP4 Antibody

  • Chronic renal failure gene protein
  • CRFGGTP-binding protein NGB
  • FLJ10686
  • FLJ10690
  • G protein-binding protein CRFG
  • GTP binding protein 4
  • NGB
  • NOG1FLJ39774
  • nucleolar GTP-binding protein 1

Background

GTP-binding proteins are GTPases and function as molecular switches that can flip between two states: active, when GTP is bound, and inactive, when GDP is bound. 'Active' in this context usually means that the molecule acts as a signal to trigger other events in the cell. When an extracellular ligand binds to a G-protein-linked receptor, the receptor changes its conformation and switches on the trimeric G proteins that associate with it by causing them to eject their GDP and replace it with GTP. The switch is turned off when the G protein hydrolyzes its own bound GTP, converting it back to GDP. But before that occurs, the active protein has an opportunity to diffuse away from the receptor and deliver its message for a prolonged period to its downstream target. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GTPBP4
Uniprot