GTPBP4 Antibody

Immunocytochemistry/Immunofluorescence: GTPBP4 Antibody [NBP2-16766] - A431 cells were fixed in iced-cold MeOH for 5 min. Green: GTPBP4 protein stained by GTPBP4 antibody diluted at 1:500. Blue: Hoechst 33343 staining.

Western Blot: GTPBP4 Antibody [NBP2-16766] - Sample (10 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:5000.

GTPBP4 antibody detects GTPBP4 protein by western blot analysis.A. 30 ug 293T whole cell lysate/extractB. 30 ug A431 whole cell lysate/extractC. 30 ug HeLa whole cell lysate/extractD. 30 ug HepG2 whole cell lysate/extract7.5 % SDS-PAGEGTPBP4 antibody (NBP2-16766) dilution: 1:5000

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GTPBP4 Antibody Summary

• Description: Novus Biologicals Rabbit GTPBP4 Antibody (NBP2-16766) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GTPBP4. The exact sequence is proprietary.
• Localization: Nucleus; nucleolus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GTPBP4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for GTPBP4 Antibody

• Chronic renal failure gene protein
• CRFGGTP-binding protein NGB
• FLJ10686
• FLJ10690
• G protein-binding protein CRFG
• GTP binding protein 4
• NGB
• NOG1FLJ39774
• nucleolar GTP-binding protein 1

Background

GTP-binding proteins are GTPases and function as molecular switches that can flip between two states: active, when GTP is bound, and inactive, when GDP is bound. 'Active' in this context usually means that the molecule acts as a signal to trigger other events in the cell. When an extracellular ligand binds to a G-protein-linked receptor, the receptor changes its conformation and switches on the trimeric G proteins that associate with it by causing them to eject their GDP and replace it with GTP. The switch is turned off when the G protein hydrolyzes its own bound GTP, converting it back to GDP. But before that occurs, the active protein has an opportunity to diffuse away from the receptor and deliver its message for a prolonged period to its downstream target. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: GTPBP4
• Uniprot:
  • Q9BZE4(Human)