Cytoglobin Antibody (1A1)

Images

 
Western Blot: Cytoglobin Antibody (1A1) [H00114757-M02] - Analysis of CYGB expression in transfected 293T cell line by CYGB monoclonal antibody (M02), clone 1A1.Lane 1: CYGB transfected lysate(21 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: Cytoglobin Antibody (1A1) [H00114757-M02] - Analysis of monoclonal antibody to CYGB on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Cytoglobin Antibody (1A1) [H00114757-M02] - Analysis of monoclonal antibody to CYGB on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.
ELISA: Cytoglobin Antibody (1A1) [H00114757-M02] - Detection limit for recombinant GST tagged CYGB is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
1A1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Cytoglobin Antibody (1A1) Summary

Immunogen
CYGB (AAH29798, 1 a.a. ~ 191 a.a) full length recombinant protein with GST tag.MEKVPGEMEIERRERSEELSEAERKAVQAMWARLYASCEDVGVAILVRFFVNFPSAKQYFSQFKHMEDPLEMERSPQLRKHACRVMGALNTVVENLHDPDKVSSVLALVGKAHALKHKVEPVYFKILSGVILEVVAEEFASDFPPETQRAWAKLRGLIYSHVTAAYKEVGWVQQVPNATTPPATLPSSGP
Specificity
CYGB - cytoglobin
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CYGB
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Antibody reactive against transfected lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin), immunofluorescence, and ELISA.
Publications
Read Publications using H00114757-M02.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Cytoglobin Antibody (1A1)

  • cytoglobin
  • HGBHistoglobin
  • histoglobin
  • STAPHGb
  • Stellate cell activation-associated protein

Background

Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues, scavenge nitric oxide or other reactive oxygen species, or serve a protective function during oxidative stress (Trent and Hargrove, 2002 [PubMed 11893755]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for Cytoglobin Antibody (H00114757-M02)(5)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CYGB
Entrez
OMIM
Uniprot