GSH2 Antibody (HL2533)

Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with GSH2 antibody [HL2533] (NBP3-48695) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Whole zebrafish extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with GSH2 antibody [HL2533] (NBP3-48695) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with GSH2 antibody [HL2533] (NBP3-48695) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

GSH2 antibody [HL2533] detects GSH2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.GSH2 stained by GSH2 antibody [HL2533] (NBP3-48695) diluted at 1:100.Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Ze
• Applications: WB, IHC
• Clone: HL2533
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

GSH2 Antibody (HL2533) Summary

• Description: Novus Biologicals Rabbit GSH2 Antibody (HL2533) (NBP3-48695) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human GSH2
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GSX2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for GSH2 Antibody (HL2533)

• GS homeobox 2
• GSH2
• Homeobox protein GSH-2

Background

The GSH2 (also known as GSX2) gene codes for a 304 amino acid long, 32 kDA GS homeobox 2 protein that is a transcription factor that binds to the DNA sequence 5'-CNAATTAG-3'. The GSH2 gene has been researched for its role in leukemia as well as neuronitis. Interactions between GSH2 and ABL1 and NKX2-1 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GSX2