GRP78/HSPA5 Antibody

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Western Blot: GRP78/HSPA5 Antibody [NBP2-42848] - Analysis of A. 50 ug Rat heart lysate/extract B. 50 ug Rat Lung lysate/extract C. 50 ug Rat Liver lysate/extract D. 50 ug Rat kidney lysate/extract 7.5 % SDS-PAGE Hspa5 ...read more
Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody [NBP2-42848] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hspa5 protein stained by Hspa5 antibody diluted at 1:500. Blue: Hoechst 33342 ...read more
Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-42848] - Paraffin-embedded rat brain. Hspa5 antibody diluted at 1:500.
Western Blot: GRP78/HSPA5 Antibody [NBP2-42848] - Analysis of 30 ug whole cell lysate. A: 293T B: A431 C: HeLa D: HepG2 E: A375 7.5% SDS PAGE diluted at 1:10000.
Western Blot: GRP78/HSPA5 Antibody [NBP2-42848] - Analysis of 30 ug whole cell lysate. A: NIH-3T-3 B: JC C: BCL-1 D: C2C12 E: Raw264.7 7.5% SDS PAGE diluted at 1:10000..
Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-42848] - Analysis of gastric carcinoma. Hspa5 antibody dilution: 1:500.
Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-42848] - Paraffin-embedded mouse liver. Hspa5 antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

GRP78/HSPA5 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse Hspa5. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPA5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for GRP78/HSPA5 Antibody

  • 78 kDa glucose-regulated protein
  • BIP
  • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
  • FLJ26106
  • GRP78
  • GRP-78
  • GRP78BIP
  • Heat shock 70 kDa protein 5
  • heat shock 70kD protein 5 (glucose-regulated protein, 78kD)
  • heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
  • HSP70-5
  • HSPA5
  • Immunoglobulin heavy chain-binding protein
  • MIF2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for GRP78/HSPA5 Antibody (NBP2-42848)

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Diseases for GRP78/HSPA5 Antibody (NBP2-42848)

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Blogs on GRP78/HSPA5.

GRP78 - molecular chaperone and negative regulator of the unfolded protein response
The 78 kDa glucose-regulated protein (GRP78) is the eukaryotic orthologue to the prokaryotic heat shock 70 kDa protein 5 (HSPA5). GRP78 is also sometimes referred to as BiP. GRP78 is a member of the HSP70 family and plays dynamic roles in protein...  Read full blog post.

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Bioinformatics

Gene Symbol HSPA5