GRP78/HSPA5 Antibody - BSA Free

Western Blot: GRP78 Antibody [NBP2-16749] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:10000.

Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Rat brain. Grp78 antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Mouse intestine. Grp78 stained by Grp78 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - GRP78/HSPA5 proteins were measured by immunoblotting in epithelioid (EPI BAP1+ UPN7), biphasic (BIP BAP1- UPN16) and sarcomatous (BAP1+ UPN22) MPM cells. Tubulin was used as a loading control. The figure is representative of 1 out of 3 experiments with similar results. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35197103/) licensed under a CC-BY license.

Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat liver.Grp78 stained by Grp78 antibody (NBP2-16749) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Grp78 antibody detects Grp78 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Grp78 antibody (NBP2-16749) diluted at 1:250. br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Grp78 antibody detects Grp78 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Grp78 protein stained by Grp78 antibody (NBP2-16749) diluted at 1:500.Blue: Hoechst 33342 staining.

Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody (NBP2-16749) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat duodenum.Grp78 stained by Grp78 antibody (NBP2-16749) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Grp78 antibody detects Grp78 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Grp78 stained by Grp78 antibody (NBP2-16749) diluted at 1:500.Red: Tau, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody (NBP2-16749) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Grp78 antibody detects Grp78 protein by western blot analysis.A. 30 ug PC-12 whole cell extract B. 30 ug Rat2 whole cell extract7.5% SDS-PAGEGrp78 antibody (NBP2-16749) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.Grp78 stained by Grp78 antibody (NBP2-16749) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt, Ch, Bv, Fi, RM, Xp, Ze
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GRP78/HSPA5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GRP78/HSPA5 Antibody - BSA Free (NBP2-16749) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-GRP78/HSPA5 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GRP78/HSPA5. The exact sequence is proprietary.
• Localization: Endoplasmic reticulum lumen; Melanosome
• Marker: ER Stress Marker
• Predicted Species: Bovine (100%), Zebrafish (95%), Rhesus Macaque (100%), Fish (95%), Xenopus (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSPA5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:5000-1:20000
• Theoretical MW: 72 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Japanese Medaka (95%), Xenopus laevis (99%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for GRP78/HSPA5 Antibody - BSA Free

• 78 kDa glucose-regulated protein
• BIP
• Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
• FLJ26106
• GRP78
• GRP-78
• GRP78BIP
• Heat shock 70 kDa protein 5
• heat shock 70kD protein 5 (glucose-regulated protein, 78kD)
• heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
• HSP70-5
• HSPA5
• Immunoglobulin heavy chain-binding protein
• MIF2

Background

When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GRP78/HSPA5 Antibody (NBP2-16749)(1)

Publication using NBP2-16749

• Salaroglio IC, Belisario DC, Bironzo P et al. SKP2 drives the sensitivity to neddylation inhibitors and cisplatin in malignant pleural mesothelioma Journal of experimental & clinical cancer research : CR 2022-02-23 [PMID: 35197103] (WB, Human)

Bioinformatics

• Gene Symbol: HSPA5
• Uniprot:
  • P11021(Human)