GRP78/HSPA5 Antibody

Images

 
Western Blot: GRP78/HSPA5 Antibody [NBP1-54318] - analysis of Human Glucose deprived glia cell lysates showing detection of GRP78 protein using Rabbit Anti-GRP78 Polyclonal Antibody. Primary Antibody: Rabbit Anti-GRP78 ...read more
Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-54318] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP78 (Bip) ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Dr, HaSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

GRP78/HSPA5 Antibody Summary

Immunogen
Full length human GRP78 (Bip) his tagged at the N terminus
Localization
Endoplasmic Reticulum, Endoplasmic Reticulum Membrane, Melanosome
Marker
ER Stress Marker
Specificity
Detects a protein with a mass of approx. 78kDa corresponding to Grp78.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPA5
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
Application Notes
0.5 ug/ml of GRP78 (Bip) Antibody was sufficient for detection of Grp78 in 10 ug of rat tissue lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary Antibody.
Publications
Read Publication using NBP1-54318.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for GRP78/HSPA5 Antibody

  • 78 kDa glucose-regulated protein
  • BIP
  • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
  • FLJ26106
  • GRP78
  • GRP-78
  • GRP78BIP
  • Heat shock 70 kDa protein 5
  • heat shock 70kD protein 5 (glucose-regulated protein, 78kD)
  • heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
  • HSP70-5
  • HSPA5
  • Immunoglobulin heavy chain-binding protein
  • MIF2

Background

GRP78 is a ubiquitously expressed, 78-kDa glucoseregulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GRP78/HSPA5 Antibody (NBP1-54318)(1)

We have publications tested in 1 confirmed species: Human.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Blogs on GRP78/HSPA5.

GRP78 - molecular chaperone and negative regulator of the unfolded protein response
The 78 kDa glucose-regulated protein (GRP78) is the eukaryotic orthologue to the prokaryotic heat shock 70 kDa protein 5 (HSPA5). GRP78 is also sometimes referred to as BiP. GRP78 is a member of the HSP70 family and plays dynamic roles in protein...  Read full blog post.

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Bioinformatics

Gene Symbol HSPA5