Growth Hormone Antibody [Biotin]

Growth Hormone was detected in perfusion fixed frozen sections of rat pituitary using Goat Anti-Rat Growth Hormone Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1566) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Growth Hormone was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Rat Growth Hormone Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1566) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Growth Hormone Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant rat Growth Hormone
  Leu27-Phe216
  Accession # P01244
• Specificity: Detects rat Growth Hormone in Western blots. In this format, approximately 10% cross-reactivity with recombinant human Growth Hormone is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GH1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Growth Hormone Antibody [Biotin]

• GH1
• GHB5
• GHIGHD1B
• GHN
• GH-N
• GHNGrowth hormone
• GH-Nsomatotropin
• growth hormone 1Pituitary growth hormone
• Growth Hormone
• hGH-N
• IGHD1A
• IGHD2
• Somatotropin

Background

Growth Hormone, also named somatotropin, belongs to the somatotropin/prolactin superfamily. It is synthesized in the anterior pituitary gland. Growth Hormone regulates body growth by stimulating the secretion of IGF-I by liver and other tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GH1
• Uniprot:
  • P01244()