Granzyme B Antibody

Immunocytochemistry/Immunofluorescence: Granzyme B Antibody [NB100-684] - Staining of Granzyme B in red on FFPE human Tonsil. Secondary antibody is donkey anti-rabbit A647. Nuclei counter-stained with DAPI. Staining pattern is cytoplasmic with obvious polarization (see arrowheads) in some cases. This image was submitted via customer Review.

Immunohistochemistry-Paraffin: Granzyme B Antibody [NB100-684] - FFPE human tonsil stained with Granzyme B antibody.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Granzyme B Antibody Summary

• Description: Novus Biologicals Rabbit Granzyme B Antibody (NB100-684) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-Granzyme B Antibody: Cited in 8 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide: EIIGGHEAKPHSRPYMAYL, corresponding to amino acids 20-38 of Human Granzyme B (N-terminus).
• Localization: Cytoplasmic
• Specificity: This reacts with a 32 kDa protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GZMB
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:100
• Application Notes: IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4), 0.2% BSA, Tween-20
• Preservative: 0.05% Sodium Azide
• Purity: Affinity purified

Alternate Names for Granzyme B Antibody

• C11
• CCPI
• CGL1
• CGL-1
• CSPB
• CSP-B
• CTLA1
• CTLA-1
• CTSGL1
• Fragmentin-2
• Granzyme B
• Granzyme-2
• GranzymeB
• GRB
• GrzB
• GZMB
• HLP
• SECT

Background

The lytic enzymes of cytotoxic lymphocytes seem to be contained within lysosome-like granules found in the cytoplasm. These granules contain perforin, a membrane pore-forming protein, and a family of serine proteases (granzymes). Granzyme B, a 32 kDa protein, is serine Aspase associated with the granules of Natural Killer (NK) cells. Granzyme B seems to be able to elicit cell death, perhaps by activating an endogenous pathway of programmed cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Granzyme B Antibody (NB100-684)(8)

Publications using NB100-684

• Chen X, Zhao J, Herjan T et al. IL-17-induced HIF1? drives resistance to anti-PD-L1 via fibroblast-mediated immune exclusion Journal of Experimental Medicine 2022-06-06 [PMID: 35389431] (Immunohistochemistry-Paraffin, Human)
• Fujiwara H, Mathew Av, Kovalneko I et Al. Mitochondrial Complex II In Intestinal Epithelial Cells Regulates T-cell Mediated Immunopathology Nat Immunol 2021-10-23 [PMID: 34686860]
• Fang W, Zhou T, Shi H, et al. Progranulin induces immune escape in breast cancer via up-regulating PD-L1 expression on tumor-associated macrophages (TAMs) and promoting CD8+ T cell exclusion Journal of experimental & clinical cancer research : CR 2021-01-04 [PMID: 33390170] (IHC-P, Mouse)
• YR Na, JW Kwon, DY Kim, H Chung, J Song, D Jung, H Quan, D Kim, JS Kim, YW Ju, W Han, HS Ryu, YS Lee, JJ Hong, SH Seok Protein Kinase A Catalytic Subunit Is a Molecular Switch that Promotes the Pro-tumoral Function of Macrophages Cell Rep, 2020-05-12;31(6):107643. 2020-05-12 [PMID: 32402274]
• Luchtel RA, Bhagat T, Pradhan K et al. High-dose ascorbic acid synergizes with anti-PD1 in a lymphoma mouse model Proc. Natl. Acad. Sci. U.S.A. 2020-01-21 [PMID: 31911474] (ICC/IF, Mouse)
• Giles AJ, Hao S, Padget M et al. Efficient ADCC- killing of meningioma by avelumab and a high-affinity natural killer cell line, haNK JCI Insight. 2019-10-17 [PMID: 31536478] (IHC-P, Human)
• Medler T, Murugan D, Horton W et al. Complement C5a Fosters Squamous Carcinogenesis and Limits T Cell Response to Chemotherapy Cancer Cell. 2018-10-08 [PMID: 30300579] (IF/IHC, IF/IHC, Mouse)
• Gunderson AJ, Kaneda MM, Tsujikawa T et al. Bruton Tyrosine Kinase-Dependent Immune Cell Cross-talk Drives Pancreas Cancer. Cancer Discov. 2016-03-06 [PMID: 26715645] (IHC-P, Human)

Review for Granzyme B Antibody (NB100-684) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Manuel Flores ICC Human 06/11/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Human Tonsil tissue
• Species: Human

Bioinformatics

• Gene Symbol: GZMB
• Entrez:
  • 3002(Human)
  • 14939(Mouse)
  • 171528(Rat)