Granzyme B Antibody

Images

 
Immunocytochemistry: Granzyme B Antibody [NB100-684] - Staining of Granzyme B in red on FFPE human Tonsil. Secondary antibody is donkey anti-rabbit A647. Nuclei countestained with DAPI. Staining pattern is cytoplasmic ...read more
Immunohistochemistry-Paraffin: Granzyme B Antibody [NB100-684] - Formalin fixed paraffin embedded human tonsil stained with Granzyme B antibody

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC, IHC-Fr, IHC-P, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Granzyme B Antibody Summary

Immunogen
Synthetic peptide: EIIGGHEAKPHSRPYMAYL, corresponding to amino acids 20-38 of Human Granzyme B.
Localization
Cytoplasmic
Specificity
This reacts with a 32 kDa protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GZMB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunocytochemistry
Application Notes
Tested applications:* Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - 1/50 - 1/100 for 30mins at RT (ABC method). Perform heat mediated antigen retrieval (using 10mM citrate buffer pH 6.0) before commencing with IHC staining protocol. Immunohistochemistry (Frozen sections) - 1/50 - 1/100 for 30mins at RT (ABC method). Fix with ice cold acetone. Immunofluorescence - 1/50 Not tested in other applications. *Optimal dilutions / concentrations should be determined by the end user. Immunohistochemistry: use at a dilution of 1/50 - 1/100 for 30mins at RT (ABC method). For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval using 10mM citrate buffer pH 6.0 before commencing with the IHC staining protocol.
Reviewed Applications
Read 1 Review rated 5
using
NB100-684 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS and BSA
Preservative
Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Granzyme B Antibody

  • C11
  • CCPI
  • CGL1
  • CGL-1
  • CSPB
  • CSP-B
  • CTLA1
  • CTLA-1
  • CTSGL1
  • Fragmentin-2
  • Granzyme B
  • Granzyme-2
  • GRB
  • GrzB
  • GZMB
  • HLP
  • SECT

Background

The lytic enzymes of cytotoxic lymphocytes seem to be contained within lysosome-like granules found in the cytoplasm. These granules contain perforin, a membrane pore-forming protein, and a family of serine proteases (granzymes). Granzyme B, a 32 kDa protein, is a serine Aspase associated with the granules of Natural Killer (NK) cells. Granzyme B seems to be able to elicit cell death, perhaps by activating an endogenous pathway of programmed cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, ICC/IF

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Review for Granzyme B Antibody (NB100-684) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-684:
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Images Ratings Applications Species Date Details
Immunocytochemistry Granzyme B NB100-684
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reviewed by:
Manuel Flores
ICC Human 06/11/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedHuman Tonsil tissue
SpeciesHuman

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Caspase 7: The Cell's Suicide Switch
Caspase 7 (also known as CASP7, Mch3, ICE-LAP3, CMH-1) is a member of caspase family of cysteine proteases. It is an apoptosis-related cystein peptidase encoded by the CASP7 gene in humans. CASP7 homologous sequences have been identified in nearly all...  Read full blog post.

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Recent Reviews

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1
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Manuel Flores
06/11/2017
Application: ICC
Species: Human

Bioinformatics

Gene Symbol GZMB
Entrez