GR/NR3C1 Antibody

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Genetic Strategies: Knockout Validated: GR/NR3C1 Antibody [NBP2-42221] - Wild-type (WT) and Glucocorticoid receptor knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was ...read more
Western Blot: GR/NR3C1 Antibody [NBP2-42221] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Glucocorticoid Receptor antibody diluted at 1:1000. The HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody [NBP2-42221] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution
Immunohistochemistry-Paraffin: GR/NR3C1 Antibody [NBP2-42221] - Human lung cancer. Glucocorticoid Receptor stained by Glucocorticoid Receptor antibody (GR/NR3C1)
Immunohistochemistry-Paraffin: GR/NR3C1 Antibody [NBP2-42221] - Human colon carcinoma, using Glucocorticoid receptorantibody at 1:250 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunoprecipitation: GR/NR3C1 Antibody [NBP2-42221] - Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of ...read more

Product Details

Summary
Reactivity Hu, Bv, FeSpecies Glossary
Applications WB, ICC/IF, IHC, IP, ChIP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

GR/NR3C1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Glucocorticoid receptor. The exact sequence is proprietary.
Localization
Cytoplasm; Nucleus
Predicted Species
Feline (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NR3C1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation Sequencing -Reported in scientific literature (PMID:35077705)
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:500-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
Theoretical MW
86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-42221 in the following applications:

Reactivity Notes

Bovine reactivity reported in scientific literature (Ruiz-Conca M et al). Mouse (89%), Rat (87%), Sheep (88%), Canine (87%), Guniea Pig (87%), Porcine (83%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 1% BSA, 20% Glycerol (pH7).
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for GR/NR3C1 Antibody

  • GCR
  • glucocorticoid receptor
  • GR
  • GRGCCR
  • GRLNuclear receptor subfamily 3 group C member 1
  • NR3C1
  • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
  • nuclear receptor subfamily 3, group C, member 1

Background

The protein encoded by this gene is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for GR/NR3C1 Antibody (NBP2-42221)(5)

We have publications tested in 2 confirmed species: Human, Bovine.

We have publications tested in 4 applications: Chemotaxis, Chip Cytometry, ICC/IF, WB.


Filter By Application
Chemotaxis
(1)
Chip Cytometry
(1)
ICC/IF
(1)
WB
(1)
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Filter By Species
Human
(2)
Bovine
(2)
All Species
Showing Publications 1 - 5 of 5.
Publications using NBP2-42221 Applications Species
Ruiz-Conca M, Gardela J, Alvarez-Rodriguez M et al. Immunofluorescence analysis of NR3C1 receptor following cortisol exposure during bovine in vitro oocyte maturation AETE Abstract 2019-09-12 (ICC/IF, Bovine) ICC/IF Bovine
Hoffman JA, Trotter KW, Day CR et al. Multimodal regulatory elements within a hormone-specific super enhancer control a heterogeneous transcriptional response Molecular cell 2022-01-20 [PMID: 35077705] (Chip Cytometry, Human) Chip Cytometry Human
Leung C, Kosyk O, Welter E et al. Naturalistic Glucocorticoid Receptor Activation Programs Key Cell Phenotypes and Functional Epigenomic Patterns in Human Fibroblasts SSRN Electronic Journal
Leung C, Kosyk O, Welter E et al. Chronic stress-driven glucocorticoid receptor activation programs key cell phenotypes and functional epigenomic patterns in human fibroblasts iScience 2022-09-01 [PMID: 36065188] (Chemotaxis, Human) Chemotaxis Human
Ruiz-Conca M, Gardela J, Mogas T et al. Apoptosis and glucocorticoid-related genes mRNA expression is modulated by coenzyme Q10 supplementation during in vitro maturation and vitrification of bovine oocytes and cumulus cells Theriogenology 2022-10-01 [PMID: 36063671] (WB, Bovine) WB Bovine

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NR3C1
Uniprot