GR/NR3C1 Antibody (S04-8C8)

Western Blot: GR/NR3C1 Antibody (S04-8C8) [NBP3-14938] - Western blot detection of GR/NR3C1 in K562, 3T3, Hela cell lysates using GR/NR3C1 Rabbit mAb (1:1000 diluted). Predicted band size: 86kDa. Observed band size: 94, 91kDa.

Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (S04-8C8) [NBP3-14938] - Immunofluorescence of GR/NR3C1 (green) in Hela cells using NBP3-14938 at dilution 1/50, and DAPI (blue)

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: S04-8C8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

GR/NR3C1 Antibody (S04-8C8) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein of mouse GR/NR3C1 (Uniprot#: P06537)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NR3C1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1/50
  • Western Blot 1/1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for GR/NR3C1 Antibody (S04-8C8)

• GCR
• glucocorticoid receptor
• GR
• GRGCCR
• GRLNuclear receptor subfamily 3 group C member 1
• NR3C1
• nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
• nuclear receptor subfamily 3, group C, member 1

Background

Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor. The corresponding gene for the glucocorticoid receptor is NR3C1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NR3C1