GR/NR3C1 Antibody (BuGR2) [PE]

Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [PE] [NB300-731PE] - An intracellular stain was performed on Jurkat cells with GR/NR3C1 (BuGR2) antibody NB300-731PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerthrin.

• Reactivity: Hu, Mu, Rt, Gp, Rb, Sh, Ye
• Applications: WB, Flow, GS, ICC/IF, IHC, IP, B/N, ChIP
• Clone: BuGR2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE

GR/NR3C1 Antibody (BuGR2) [PE] Summary

• Immunogen: Partially purified rat GR.
• Localization: Cytoplasmic in the absence of ligand; nuclear after ligand-binding.
• Specificity: Using enzymatic digestion analysis, has been shown to react with the undigested 97 kDa GR, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NR3C1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Chromatin Immunoprecipitation (ChIP)
  • Flow Cytometry
  • Gel Super Shift Assays
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for GR/NR3C1 Antibody (BuGR2) [PE]

• GCR
• glucocorticoid receptor
• GR
• GRGCCR
• GRLNuclear receptor subfamily 3 group C member 1
• NR3C1
• nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
• nuclear receptor subfamily 3, group C, member 1

Background

Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor. The corresponding gene for the glucocorticoid receptor is NR3C1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GR/NR3C1 Antibody (NB300-731PE)(2)

Publications using NB300-731PE

• Rudak PT, Choi J, Parkins KM et al. Chronic stress physically spares but functionally impairs innate-like invariant T cells Cell Rep 2021-04-14 [PMID: 33852855]
  
  Details:
  Citation using the PE format of this antibody.
• Roh Kyung-Baeg, Park Deokhoon, Jung Eunsun et al Inhibitory Effects of Prunella vulgaris L. Extract on 11 beta-HSD1 in Human Skin Cells. Evid Based Complement Alternat Med. 2018-10-04 [PMID: 30402116] (ICC/IF, Human)
  
  Details:
  Citation using the PE version of this antibody.

FAQs for GR/NR3C1 Antibody (NB300-731PE). (Showing 1 - 1 of 1 FAQs).

1. I'm wondering if you can tell me more about your post-conjugation Q/C process. What kind of Q/C tests do you run, and how do you determine whether the conjugation is good or bad (or said another way, passes or fails)?
   • We QC test every newly produced lot of antibody (unconjugated). Once the unconjugated product meets our testing standards, we release that antibody for sale. When it comes to the conjugations, we are not able to individually test every single conjugation in each application, as these are made-to-order (we only conjugate what is needed to fulfill customer orders). For PE specifically, we perform a 3:1 ratio of dye to protein, purify via HLPC, calculate the F/P ratio, and concentrate/calculate end concentration. As long as the conjugated antibody meets our F/P ratio standards and concentration standards, we release it for shipment.

Bioinformatics

• Gene Symbol: NR3C1
• Entrez:
  • 2908(Human)
  • 14815(Mouse)
  • 24413(Rat)