Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [PE] [NB300-731PE] - An intracellular stain was performed on Jurkat cells with GR/NR3C1 (BuGR2) antibody NB300-731PE (blue) and a matched isotype control (orange). Cells were ...read more
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at 4C in the dark.
0.05% Sodium Azide
Protein A purified
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for GR/NR3C1 Antibody (BuGR2) [PE]
GRLNuclear receptor subfamily 3 group C member 1
nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
nuclear receptor subfamily 3, group C, member 1
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for GR/NR3C1 Antibody (NB300-731PE). (Showing 1 - 1 of 1 FAQ).
I'm wondering if you can tell me more about your post-conjugation Q/C process. What kind of Q/C tests do you run, and how do you determine whether the conjugation is good or bad (or said another way, passes or fails)?
We QC test every newly produced lot of antibody (unconjugated). Once the unconjugated product meets our testing standards, we release that antibody for sale. When it comes to the conjugations, we are not able to individually test every single conjugation in each application, as these are made-to-order (we only conjugate what is needed to fulfill customer orders). For PE specifically, we perform a 3:1 ratio of dye to protein, purify via HLPC, calculate the F/P ratio, and concentrate/calculate end concentration. As long as the conjugated antibody meets our F/P ratio standards and concentration standards, we release it for shipment.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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