GPIHBP1 Antibody - BSA Free

Western Blot: GPIHBP1 Antibody [NB110-55293] - Detection of HDLBP in CHO transfected lysate using NB110-55293. Lane 1: empty vector Lane 2: HDLBP transfected lysate

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

GPIHBP1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GPIHBP1 Antibody - BSA Free (NB110-55293) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide corresponding to an internal region [within residues 100-180] of human GPIHBP1. [Swiss-Prot# Q8IV16]
• Localization: Cytoplasmic and Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPIHBP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/ml
• Application Notes: This GPIHBP1 antibody is useful for Western blot analysis where a band is seen at ~19 kDa using CHO pgsA745 transfected cell lysates.
• Control Peptide:

  GPIHBP1 Peptide (NB110-55293PEP)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GPIHBP1 Antibody - BSA Free

• glycosylphosphatidylinositol anchored high density lipoprotein binding protein1
• glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein1
• GPI anchored high density lipoprotein binding protein 1
• GPI-Anchored HDL-Binding Protein 1
• GPIHBP1
• GPI-HBP1
• GPI-HBP1LOC338328
• HBP1
• High density lipoprotein-binding protein 1
• HYPL1D

Background

GPIHBP1 (glycosylphosphatidylinositol-anchored HDL-binding protein 1) is a glycosylphosphatidylinositol-anchored glycoprotein of capillary endothelial cells that shuttle lipoprotein lipase (LPL) from the interstitial spaces to capillary lumen, and is essential for triglyceride-rich lipoproteins metabolism in mammalian plasma. GPIHBP1 is localized on luminal/abluminal capillary endothelial cell surfaces where it is bound by a glycosylphosphatidylinositol anchor and associates strongly with LPL. It serves as LPL transporter from sub-endothelial spaces to luminal face of capillaries, enabling lipolysis of circulating triglycerides localized within plasma chylomicrons. It has high affinity for HDL and binds to LPL, chylomicrons as well as APOA5. In the absence of GPIHBP1, the stores of catalytically active LPL within tissues are normal, but the LPL is mislocalized to interstitial spaces and is absent from capillary lumen. LPL mislocalization interferes with lipoprotein lipolysis and causes chylomicronemia. In humans, loss of function GPIHBP1 mutations leads to familial chylomicronemia. It binds LPL and apoA-V strongly for serving as a platform for lipolysis within capillaries, particularly in tissues which show high expression levels for both GPIHBP1 and LPL genes, such as heart, skeletal muscle and adipose tissue. Gpihbp1-/Gpihbp1- knock out mice have shown that GPIHBP1-deficiency causes severe hypertriglyceridemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPIHBP1