GPAM Antibody

Images

 
Western Blot: GPAM Antibody [NBP1-76907] - Western blot analysis of GPAT1 in rat brain tissue lysate with GPAT1 antibody at (A) 1 and (B) 2 ug/mL.
Immunohistochemistry-Paraffin: GPAM Antibody [NBP1-76907] - Staining of GPAT1 in mouse brain tissue with GPAT1 antibody at 5 ug/ml.
Immunohistochemistry-Paraffin: GPAM Antibody [NBP1-76907] - Rat brain with GPAM antibody at 2.5 ug/ml.
Immunofluorescence: GPAM Antibody [NBP1-76907] - Mouse brain tissue with GPAT1 antibody at 20 ug/m, Green: GPAT1 Antibody Blue: DAPI staining
Immunofluorescence: GPAM Antibody [NBP1-76907] - Immunofluorescence of GPAT1 in Rat Brain tissue with GPAT1 antibody at 20 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

GPAM Antibody Summary

Immunogen
Antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human GPAT1. The immunogen is located within amino acids 730 - 780 of GPAT1. Amino Acid Squence: SGPVPEPEYLQKLHK
Specificity
At least four isoforms of GPAT1 are known to exist; this antibody will detect only the two largest isoforms.
Predicted Species
Porcine (100%), Bovine (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GPAM
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA 1:100-1:2000
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Immunofluorescence 20 ug/ml
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
GPAM Peptide (NBP1-76907PEP)
Publications
Read Publication using
NBP1-76907 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for GPAM Antibody

  • EC 2.3.1
  • EC 2.3.1.15
  • glycerol-3-phosphate acyltransferase 1, mitochondrial
  • glycerol-3-phosphate acyltransferase, mitochondrial
  • GPAT
  • GPAT-1
  • GPAT1mitochondrial
  • MGC26846

Background

Glycerol-3-phosphate acyltransferase 1 (GPAT1), one of four known GPAT isoforms, is located on the mitochondrial outer membrane, allowing reciprocal regulation with carnitine palmitoyltransferase-1. It is thought to be critical for the development of hepatic steatosis; steatosis triggered by GPAT1 overexpression leads to hepatic and possibly peripheral insulin resistance. GPAT1 is transcriptionally upregulated by insulin and sterol regulatory element binding protein (SREBP-1) and downregulated by AMP-activated protein kinase. Mice deficient in GPAT1 exhibit decreased triacylglycerol (TAG) in cardiomyocytes even in high-fat diets, suggesting that GPAT1 contributes significantly to TAG accumulation in heart tissue during lipogenic or high fat diets. At least two isoforms of GPAT1 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GPAM Antibody (NBP1-76907)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Mouse
(1)
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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol GPAM
Uniprot