gp96/HSP90B1/GRP94 Antibody (2H3)

Images

 
Western Blot: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - PAGE, transferred to PVDF membrane and probed with anti-human TRA 1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated ...read more
Immunocytochemistry/ Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - Staining of human ES cell colony with monoclonal anti-human TRA1 antibody (2H3)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
2H3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

gp96/HSP90B1/GRP94 Antibody (2H3) Summary

Immunogen
Recombinant human TRA1 (676-803aa) purified from E. coli
Marker
ER Stress Marker
Specificity
Anti-human TRA1 mAb, clone 2H3, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TRA1 protein.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HSP90B1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:200
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Immunofluorescence staining of human ES cell colony with monoclonal anti-human TRA1 antibody (2H3)
Control
gp96/HSP90B1/GRP94 Lysate (NBP2-65662)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for gp96/HSP90B1/GRP94 Antibody (2H3)

  • 94 kDa glucose-regulated protein
  • ECGP
  • Endoplasmin
  • endothelial cell (HBMEC) glycoprotein
  • glucose regulated protein, 94 kDa
  • gp96
  • GP96ECGP
  • Grp94
  • GRP-94
  • GRP94gp96 homolog
  • Heat shock protein 90 kDa beta member 1
  • heat shock protein 90kDa beta (Grp94), member 1
  • HSP90B1
  • TRA1endoplasmin
  • tumor rejection antigen (gp96) 1
  • Tumor rejection antigen 1
  • Tumor rejection antigen-1 (gp96)

Background

Tumor rejection antigen 1(TRA1) is expressed on the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). TRA1 is a marker protein of these stem cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-04346AF405
Alexa Fluor 488 Labeled NBP1-04346AF488
Alexa Fluor 647 Labeled NBP1-04346AF647
Alexa Fluor 700 Labeled NBP1-04346AF700
DyLight 350 Labeled NBP1-04346UV
DyLight 405 Labeled NBP1-04346V
DyLight 488 Labeled NBP1-04346G
DyLight 680 Labeled NBP1-04346FR
DyLight 755 Labeled NBP1-04346IR

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Bioinformatics Tool for gp96/HSP90B1/GRP94 Antibody (NBP1-04346)

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Diseases for gp96/HSP90B1/GRP94 Antibody (NBP1-04346)

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PTMs for gp96/HSP90B1/GRP94 Antibody (NBP1-04346)

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Bioinformatics

Gene Symbol HSP90B1
Uniprot