gp96/HSP90B1/GRP94 Antibody (2H3) - BSA Free

Western Blot: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - PAGE, transferred to PVDF membrane and probed with anti-human TRA 1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - Staining of human ES cell colony with monoclonal anti-human TRA1 antibody (2H3)

Flow Cytometry: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - Analysis of TRA1 in U87MG cell line, staining at 2-5ug for 1x106cells (red line). Thesecondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 2H3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

gp96/HSP90B1/GRP94 Antibody (2H3) - BSA Free Summary

• Immunogen: Recombinant human gp96/HSP90B1/GRP94 (676-803aa) purified from E. coli
• Marker: ER Stress Marker
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSP90B1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 2-5ug- 1-106cells
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:100-1:200

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for gp96/HSP90B1/GRP94 Antibody (2H3) - BSA Free

• 94 kDa glucose-regulated protein
• ECGP
• Endoplasmin
• endothelial cell (HBMEC) glycoprotein
• glucose regulated protein, 94 kDa
• gp96
• GP96ECGP
• Grp94
• GRP-94
• GRP94gp96 homolog
• Heat shock protein 90 kDa beta member 1
• heat shock protein 90kDa beta (Grp94), member 1
• HSP90B1
• TRA1endoplasmin
• tumor rejection antigen (gp96) 1
• Tumor rejection antigen 1
• Tumor rejection antigen-1 (gp96)

Background

Tumor rejection antigen 1(TRA1) is expressed on the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). TRA1 is a marker protein of these stem cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for gp96/HSP90B1/GRP94 Antibody (NBP1-04346)(1)

Publication using NBP1-04346

• Mariscal J, Fernandez-Puente P, Calamia V et al. Proteomic characterization of epithelial-like extracellular vesicles in advanced endometrial cancer. J. Proteome Res. 2018-12-26 [PMID: 30585730] (WB, Human)

Bioinformatics

• Gene Symbol: HSP90B1
• Uniprot:
  • NP_003290(Human)