GOLM1 Antibodies
We offer GOLM1 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Sandwich ELISA, Western Blot. Each GOLM1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our GOLM1 Antibodies can be used in a variety of model species: Human, Mouse, Primate, Rat. Use the list below to choose the GOLM1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our GOLM1 polyclonal antibodies and browse our GOLM1 monoclonal antibody catalog.
Alternate Names for GOLM1 Antibodies
anti-GOLM1 antibody, anti-bA379P1.3 antibody, anti-FLJ23608 antibody, anti-golgi membrane protein 1 antibody, anti-Golgi membrane protein GP73 antibody, anti-Golgi phosphoprotein 2C9orf155 antibody, anti-golgi protein, 73-kD antibody, anti-GOLPH2 FLJ22634 antibody, anti-GP73 chromosome 9 open reading frame 155 antibody, anti-PSEC0257 antibody
22 Results for "golm1" in Primary Antibodies
![Western Blot: GOLM1 Antibody [NBP1-50627] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and GOLM1 knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1:5,000 of Rabbit Anti-Human GOLM1 polyclonal Antibody (Catalog # NBP1-50627) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for GOLM1 at approximately 75 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Knockout-Validated-NBP1-50627-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GOLM1 Antibody [NBP1-50627] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and GOLM1 knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1:5,000 of Rabbit Anti-Human GOLM1 polyclonal Antibody (Catalog # NBP1-50627) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for GOLM1 at approximately 75 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: GOLM1 Antibody [NBP1-50627] - Western Blot Image of GOLM1. Whole cell protein from HeLa (lane 1), 3T3 (lane 2), MEF (lane 3), Raw264.7 (lane 4), L929 (lane 5), Neuro2A (lane 6) and PC12 (lane 7) were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 mg/ml anti-GOLM1 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Western-Blot-NBP1-50627-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GOLM1 Antibody [NBP1-50627] - Confocal immunofluorescent analysis of HeLa cells using GOLM1 antibody (NBP1-50627, 1:25). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/GOLM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-50627-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: GOLM1 Antibody [NBP1-50627] - ICC staining of GOLM1 in HEK293T cells with FITC (green). Nuclei (blue) were counterstained with Dapi.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-50627-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GOLM1 Antibody [NBP1-50627] - GOLM1 antibody was tested in HEK-293 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/GOLM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-50627-img0003.jpg)
![Western Blot: GOLM1 Antibody (OTI4B12) [NBP2-01639] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GOLM1 monoclonal antibody.](http://images.novusbio.com/fullsize/GOLM1-Antibody-OTI4B12-Western-Blot-NBP2-01639-img0009.jpg "Western Blot: GOLM1 Antibody (OTI4B12) [NBP2-01639] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GOLM1 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: GOLM1 Antibody (OTI4B12) [NBP2-01639] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GOLM1.](http://images.novusbio.com/fullsize/GOLM1-Antibody-OTI4B12-Immunocytochemistry-Immunofluorescence-NBP2-01639-img0008.jpg)
![Immunohistochemistry-Paraffin: GOLM1 Antibody (OTI4B12) [NBP2-01639] - Staining of paraffin-embedded Human prostate tissue using anti-GOLM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GOLM1-Antibody-OTI4B12-Immunohistochemistry-Paraffin-NBP2-01639-img0007.jpg)
![Flow Cytometry: GOLM1 Antibody (OTI4B12) [NBP2-01639] - Analysis of Hela cells, using anti-GOLM1 antibody , (Red), compared to a nonspecific negative control antibody, (Blue).](http://images.novusbio.com/fullsize/GOLM1-Antibody-OTI4B12-Flow-Cytometry-NBP2-01639-img0010.jpg)
![Western Blot: GOLM1 Antibody (OTI4B12) [NBP2-01639] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GOLM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GOLM1.](http://images.novusbio.com/fullsize/GOLM1-Antibody-OTI4B12-Western-Blot-NBP2-01639-img0001.jpg)
![Immunohistochemistry-Paraffin: GOLM1 Antibody (OTI4B12) [NBP2-01639] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GOLM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GOLM1-Antibody-OTI4B12-Immunohistochemistry-Paraffin-NBP2-01639-img0002.jpg)
![Immunohistochemistry-Paraffin: GOLM1 Antibody (OTI4B12) [NBP2-01639] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GOLM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GOLM1-Antibody-OTI4B12-Immunohistochemistry-Paraffin-NBP2-01639-img0003.jpg)
![Immunohistochemistry-Paraffin: GOLM1 Antibody (OTI4B12) [NBP2-01639] - Staining of paraffin-embedded Human colon tissue using anti-GOLM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GOLM1-Antibody-OTI4B12-Immunohistochemistry-Paraffin-NBP2-01639-img0004.jpg)
![Immunohistochemistry-Paraffin: GOLM1 Antibody (OTI4B12) [NBP2-01639] - Staining of paraffin-embedded Human endometrium tissue using anti-GOLM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GOLM1-Antibody-OTI4B12-Immunohistochemistry-Paraffin-NBP2-01639-img0005.jpg)
![Immunohistochemistry-Paraffin: GOLM1 Antibody (OTI4B12) [NBP2-01639] - Staining of paraffin-embedded Human Kidney tissue using anti-GOLM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GOLM1-Antibody-OTI4B12-Immunohistochemistry-Paraffin-NBP2-01639-img0006.jpg)
![Western Blot: GOLM1 Antibody (5B10) [H00051280-M06] - Analysis of GOLM1 expression in transfected 293T cell line by GOLM1 monoclonal antibody (M06), clone 5B10. Lane 1: GOLM1 transfected lysatE (45.3 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GOLM1-Antibody-5B10-Western-Blot-H00051280-M06-img0005.jpg "Western Blot: GOLM1 Antibody (5B10) [H00051280-M06] - Analysis of GOLM1 expression in transfected 293T cell line by GOLM1 monoclonal antibody (M06), clone 5B10. Lane 1: GOLM1 transfected lysatE (45.3 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: GOLM1 Antibody (5B10) [H00051280-M06] - Analysis of monoclonal antibody to GOLM1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/GOLM1-Antibody-5B10-Immunocytochemistry-Immunofluorescence-H00051280-M06-img0003.jpg)
![Immunohistochemistry-Paraffin: GOLM1 Antibody (5B10) [H00051280-M06] - Analysis of monoclonal antibody to GOLM1 on formalin-fixed paraffin-embedded human endometrium. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/GOLM1-Antibody-5B10-Immunohistochemistry-Paraffin-H00051280-M06-img0004.jpg)
![ELISA: GOLM1 Antibody (5B10) [H00051280-M06] - Detection limit for recombinant GST tagged GOLPH2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GOLM1-Antibody-5B10-ELISA-H00051280-M06-img0002.jpg)
![Immunohistochemistry-Paraffin: GOLM1 Antibody [NBP1-88775] - Staining in human stomach and skeletal muscle tissues using anti-GOLM1 antibody. Corresponding GOLM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Immunohistochemistry-Paraffin-NBP1-88775-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GOLM1 Antibody [NBP1-88775] - Staining in human stomach and skeletal muscle tissues using anti-GOLM1 antibody. Corresponding GOLM1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: GOLM1 Antibody [NBP1-88775] - Analysis in human cell line NTERA-2.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Western-Blot-NBP1-88775-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: GOLM1 Antibody [NBP1-88775] - Staining of human cell line A-431 shows positivity in golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88775-img0004.jpg)
![Immunohistochemistry-Paraffin: GOLM1 Antibody [NBP1-88775] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Immunohistochemistry-Paraffin-NBP1-88775-img0008.jpg)
![Immunohistochemistry-Paraffin: GOLM1 Antibody [NBP1-88775] - Staining of human stomach shows high expression.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Immunohistochemistry-Paraffin-NBP1-88775-img0009.jpg)
![Western Blot: GOLM1 Antibody [NBP1-88774] - Analysis in control (vector only transfected HEK293T lysate) and GOLM1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/GOLM1-Antibody-Western-Blot-NBP1-88774-img0010.jpg "Western Blot: GOLM1 Antibody [NBP1-88774] - Analysis in control (vector only transfected HEK293T lysate) and GOLM1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: GOLM1 Antibody [NBP1-88774] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88774-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GOLM1 Antibody [NBP1-88774] - Staining in human stomach and skeletal muscle tissues using anti-GOLM1 antibody. Corresponding GOLM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Immunohistochemistry-Paraffin-NBP1-88774-img0008.jpg)
![Western Blot: GOLM1 Antibody [NBP1-88774] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Western-Blot-NBP1-88774-img0006.jpg)
![Immunohistochemistry-Paraffin: GOLM1 Antibody [NBP1-88774] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Immunohistochemistry-Paraffin-NBP1-88774-img0005.jpg)
![Immunohistochemistry-Paraffin: GOLM1 Antibody [NBP1-88774] - Staining of human stomach shows high expression.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Immunohistochemistry-Paraffin-NBP1-88774-img0007.jpg)
![Western Blot: GOLM1 Antibody [NBP1-77079] - Rat brain tissue lysate with GOLM1 antibody at (A) 0.25 and (B) 0.5 ug/mL.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Western-Blot-NBP1-77079-img0001.jpg "Western Blot: GOLM1 Antibody [NBP1-77079] - Rat brain tissue lysate with GOLM1 antibody at (A) 0.25 and (B) 0.5 ug/mL.")
![Immunohistochemistry: GOLM1 Antibody - BSA Free [NBP1-77079] - Immunohistochemistry of GOLM1 in rat brain tissue with GOLM1 antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77079_rabbit-polyclonal-golm1-antibody-271120241956211.jpg)
![Immunocytochemistry/ Immunofluorescence: GOLM1 Antibody - BSA Free [NBP1-77079] - Immunofluorescence of GOLM1 in rat brain tissue with GOLM1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77079_rabbit-polyclonal-golm1-antibody-2711202420405516.jpg)
![Immunocytochemistry/Immunofluorescence: GOLM1 Antibody (12) [NBP3-12533] - Immunofluorescence staining of GOLM1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-human GOLM1 monoclonal antibody (dilution ratio 1:60) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to golgi apparatus .](http://images.novusbio.com/fullsize/GOLM1-Antibody-12-Immunocytochemistry-Immunofluorescence-NBP3-12533-img0001.jpg "Immunocytochemistry/Immunofluorescence: GOLM1 Antibody (12) [NBP3-12533] - Immunofluorescence staining of GOLM1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-human GOLM1 monoclonal antibody (dilution ratio 1:60) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to golgi apparatus .")
![Immunohistochemistry-Paraffin: GOLM1 Antibody (12) [NBP3-12533] - Immunochemical staining of human GOLM1 in human liver (cirrhosis) with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections)](http://images.novusbio.com/fullsize/GOLM1-Antibody-12-Immunohistochemistry-Paraffin-NBP3-12533-img0004.jpg)
![Immunohistochemistry-Paraffin: GOLM1 Antibody (12) [NBP3-12533] - Immunochemical staining of human GOLM1 in human uterus with mouse monoclonal antibody (8 ug/mL, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/GOLM1-Antibody-12-Immunohistochemistry-Paraffin-NBP3-12533-img0002.jpg)
![Immunohistochemistry-Paraffin: GOLM1 Antibody (12) [NBP3-12533] - Immunochemical staining of human GOLM1 in human stomach with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/GOLM1-Antibody-12-Immunohistochemistry-Paraffin-NBP3-12533-img0003.jpg)
![Western Blot: GOLM1 Antibody (2Q4F0) [NBP3-33189] - Western blot analysis of various lysates using GOLM1 Rabbit mAb at 1: 1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3min.](http://images.novusbio.com/fullsize/nbp3-33189_rabbit-golm1-mab-2q4f0-1012202411531023.jpg "Western Blot: GOLM1 Antibody (2Q4F0) [NBP3-33189] - Western blot analysis of various lysates using GOLM1 Rabbit mAb at 1: 1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3min.")
![Western Blot: GOLM1 Antibody (9C2) [NBP3-26550] - Positive Western Blot detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate. All lanes: GOLM1 Antibody at 1.3ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46, 45 KDa Observed band size: 80 KDa](http://images.novusbio.com/fullsize/nbp3-26550_rabbit-golm1-mab-9c2-28220241136308.jpg "Western Blot: GOLM1 Antibody (9C2) [NBP3-26550] - Positive Western Blot detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate. All lanes: GOLM1 Antibody at 1.3ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46, 45 KDa Observed band size: 80 KDa")
![Flow Cytometry: GOLM1 Antibody (9C2) [NBP3-26550] - Overlay histogram showing Hela cells stained with GOLM1 Antibody (9C2) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26550_rabbit-golm1-mab-9c2-282202414312724.jpg)
![Immunohistochemistry-Paraffin: GOLM1 Antibody (037) [NBP2-90166] - Immunochemical staining of human GOLM1 in human liver with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to cytoplasm of hepatocyte and interlobular bile duct. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).](http://images.novusbio.com/fullsize/GOLM1-Antibody-037-Immunohistochemistry-Paraffin-NBP2-90166-img0002.jpg "Immunohistochemistry-Paraffin: GOLM1 Antibody (037) [NBP2-90166] - Immunochemical staining of human GOLM1 in human liver with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to cytoplasm of hepatocyte and interlobular bile duct. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).")
![Immunohistochemistry-Paraffin: GOLM1 Antibody (037) [NBP2-90166] - Immunochemical staining of human GOLM1 in human uterus with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to cytoplasm of uterine gland. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).](http://images.novusbio.com/fullsize/GOLM1-Antibody-037-Immunohistochemistry-Paraffin-NBP2-90166-img0001.jpg)
![Flow Cytometry: GOLM1 Antibody (024) [NBP2-90165] - Analysis of Human GOLM1(GP73) expression on HeLa cells. The cells were treated , stained with purified anti-Human GOLM1(GP73), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/GOLM1-Antibody-024-Flow-Cytometry-NBP2-90165-img0001.jpg "Flow Cytometry: GOLM1 Antibody (024) [NBP2-90165] - Analysis of Human GOLM1(GP73) expression on HeLa cells. The cells were treated , stained with purified anti-Human GOLM1(GP73), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Flow Cytometry: GOLM1 Antibody (015) [NBP2-90164] - Analysis of Human GOLM1(GP73) expression on HeLa cells. The cells were treated, stained with purified anti-Human GOLM1(GP73), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/GOLM1-Antibody-015-Flow-Cytometry-NBP2-90164-img0001.jpg "Flow Cytometry: GOLM1 Antibody (015) [NBP2-90164] - Analysis of Human GOLM1(GP73) expression on HeLa cells. The cells were treated, stained with purified anti-Human GOLM1(GP73), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")