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GRASP55 Antibody (NBP1-89747) (2)
Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 1 application: WB.
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Reviews using NBP1-89747:
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Prathyush Pothukuchi WB
Application Western Blot Sample Tested Cell lysate from HeLa cells Species Human Lot A103873
Application Western Blot Sample Tested Hela Cell Lysate Species Human Lot A103873 Blocking
Blocking Details TBST with 5% w/v nonfat dry milk for 1 hr at room temperature. Primary Anitbody
Dilution Ratio NBP1-89747 antibody (1:2000 dilution) in TBST with 5% BSA incubated with gentle agitation overnight at 4°C Secondary Antibody
Secondary Description Goat Anti-Rabbit IgG, H & L Chain Specific Peroxidase Conjugate 1:10000 diluted in 10 ml TBST with 5% w/v nonfat dry milk Secondary Manufacturer Cat# Cat No: 401315 Merckmillipore Secondary Concentration 1:10000 Details
Detection Notes Luminata Crescendo Western HRP substrate (WBLUR0100 Merckmillipore) for 5 min at RT,exposed the x-ray film for 30 sec.
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