GM130/GOLGA2 Antibody [Janelia Fluor® 669]

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Janelia Fluor 669

GM130/GOLGA2 Antibody [Janelia Fluor® 669] Summary

• Immunogen: Partial recombinant human GM130/GOLGA2 protein (amino acids 528-606). [UniPro Q08379]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GOLGA2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for GM130/GOLGA2 Antibody [Janelia Fluor® 669]

• 130 kDa cis-Golgi matrix protein
• GM130 autoantigen
• GM130
• GM130Golgi matrix protein GM130
• GOLGA2
• golgi autoantigen, golgin subfamily a, 2
• golgin A2
• Golgin subfamily A member 2
• Golgin-95
• MGC20672
• SY11 Protein

Background

Golgi Matrix Protein (GM130) is peripheral cytoplamic protein that is bound to the Golgi membranes. It has been implicated as a maintainer of cis-Golgi structure (1). During mitosis, it regulates the disassembly and reassembly of the Golgi complex. It is also linked to docking and fusion of coatomer (COP I) coated vesicles to the Golgi membrane (2). The COOH-terminal domain of GM130 is highly homologous to Golgi human auto-antigen, golgin-95. GM130 interacts specifically and GTP-dependent manner with Rab1b protein, a regulator of anterograde traffic between ER and Golgi membranes (3). GM130 is also activator of YSK1, an essential player in cell migration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GOLGA2