Recombinant Mouse GM-CSF Protein

Product Discontinued

Recombinant Mouse GM-CSF Protein Summary

• Description: Recombinant biologically active CSF2 protein.

  Source:E. coli

• Preparation Method: GM-CSF quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.765 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GM-CSF as a Reference Standard.
• Details of Functionality: Murine GM-CSF is fully biologically active when compared to standard.The ED50 as determined by the dose-dependant stimulation of the proliferation of murine FDC-P1 cell line is less than 0.2 ng/ml, corresponding to a Specific Activity of 5x10^6 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CSF2
• Purity: >50% SDS-PAGE
• Endotoxin Note: Less than 0.1 ng/ug (IEU/ug) of Murine GM-CSF .

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 14.26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: GM-CSF Mouse was lyophilized with no additives.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >50% SDS-PAGE
• Reconstitution Instructions: Reconstitute with 20mM AcOH (acetic Acid) not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Notes

After reconstitution, store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Alternate Names for Recombinant Mouse GM-CSF Protein

• colony stimulating factor 2 (granulocyte-macrophage)
• Colony-stimulating factor
• CSF
• CSF2
• CSF-2
• GMCSF
• GM-CSF
• GMCSFgranulocyte-macrophage colony-stimulating factor
• granulocyte-macrophage colony stimulating factor
• MGC131935
• MGC138897
• Molgramostim
• molgramostin
• Sargramostim

Background

GMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CSF2