Human GM-CSF ELISA Kit (Colorimetric)

ELISA: Human GM-CSF ELISA Kit (Colorimetric) [NBP1-91277] - Representative standard curve.

Product Discontinued

Human GM-CSF ELISA Kit (Colorimetric) Summary

• Specificity: The assay detects both natural and recombinant human GM-CSF. The interference of circulating factors of the immune systemwas evaluated by spiking these proteins at physiologically relevant concentrations into a human GM-CSF positive serum. There was no crossreactivity detected.
• Standard Curve Range: 7.8 - 500 pg/mL
• Sensitivity: 2.9 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 4.40
• Intra-Assay: %CV < 6.00
• Spike Recovery: 85%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CSF2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human GM-CSF ELISA Kit (Colorimetric)

• colony stimulating factor 2 (granulocyte-macrophage)
• Colony-stimulating factor
• CSF
• CSF2
• CSF-2
• GMCSF
• GM-CSF
• GMCSFgranulocyte-macrophage colony-stimulating factor
• granulocyte-macrophage colony stimulating factor
• MGC131935
• MGC138897
• Molgramostim
• molgramostin
• Sargramostim

Background

Granulocyte - macrophage colony - stimulating factor (GM-CSF) is a small glycoprotein growth factor which stimulates the production and function of neutrophils, eosinophils and monocytes GM-CSF can be produced by a wide variety of tissue types, including fibroblasts, endothelial cells, T-cells, macrophages, mesothelial cells, epithelial cells and many types of tumor cells. In most of these tissues, inflammatory mediators, such as interleukin-1, interleukin-6, tumor necrosis factor or endotoxin, are inducers of GM-CSF gene expression, which occurs at least partly by post-transcriptional modification of the GM-CSF mRNA. The biological effects of GM-CSF are mediated through binding to cell surface receptors, which appear to be widely expressed by hematopoietic cells and also by some non-hematopoietic cells, such as endothelial cells. At least two different functional classes of GM-CSF receptor have been identified. The neutrophil GM-CSF receptor exclusively binds GM-CSF, while interleukin-3 competes for binding of GM-CSF to a second class of receptors detected on some leukemic cell lines. Monitoring of GM-CSF has been found relevant in human prostate cancer, poorly healing wounds, thyroid carcinoma, severe mucositis, fungal infections, AIDS, bone marrow transplantation, renal cell carcinoma and prostate cancer, acute lymphoblastic leukemia pulmonary inflammation, hematological malignancies, infection, lung cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for GM-CSF ELISA Kit (NBP1-91277)(1)

Publication using NBP1-91277

• Austin SA, Santhanam AV, Hinton DJ et al. Endothelial nitric oxide deficiency promotes Alzheimer's disease pathology. J Neurochem 2013-06-08 [PMID: 23745722] (ELISA)

FAQs for GM-CSF ELISA Kit (NBP1-91277). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CSF2