Human, Mouse GM-CSF ELISA Kit (Colorimetric)

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Human, Mouse GM-CSF ELISA Kit [NBP1-91277] - Data for Human GM-CSF Platinum ELISA

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, heparin)
Standard Curve Range
7.8 - 500 pg/ml
Sensitivity
2.9 pg/mL

Order Details

Human, Mouse GM-CSF ELISA Kit (Colorimetric) Summary

Standard Curve Range
7.8 - 500 pg/ml
Sensitivity
2.9 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
4.40%
Intra-Assay
6.00%
Spike Recovery
85%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CSF2

Applications/Dilutions

Application Notes
The human GM-CSF ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human GM-CSF
Publications
Read Publication using
NBP1-91277 in the following applications:

Reactivity Notes

Human. Mouse reactivity reported in the scientific literature (PMID: 23745722).

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Stop Solution (1M Phosphoric acid)
  2. Sample Diluent (Use when an external predilution of the samples is needed)
  3. Substrate Solution (tetramethyl-benzidine)
  4. Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
  5. Aluminium pouch(es) with a human GM-CSF Standard curve (coloured)
  6. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human GM-CSF, Biotin-Conjugate (anti-human GM-CSF monoclonal antibody), Streptavidin-HRP and Sample Diluent, lyophilized
  7. Adhesive Films
  8. Blue-Dye
  9. Green-Dye

Alternate Names for Human, Mouse GM-CSF ELISA Kit (Colorimetric)

  • colony stimulating factor 2 (granulocyte-macrophage)
  • Colony-stimulating factor
  • CSF
  • CSF2
  • GMCSF
  • GM-CSF
  • GMCSFgranulocyte-macrophage colony-stimulating factor
  • granulocyte-macrophage colony stimulating factor
  • MGC131935
  • MGC138897
  • molgramostin
  • sargramostim

Background

Granulocyte - macrophage colony - stimulating factor (GM-CSF) is a small glycoprotein growth factor which stimulates the production and function of neutrophils, eosinophils and monocytes GM-CSF can be produced by a wide variety of tissue types, including fibroblasts, endothelial cells, T-cells, macrophages, mesothelial cells, epithelial cells and many types of tumor cells. In most of these tissues, inflammatory mediators, such as interleukin-1, interleukin-6, tumor necrosis factor or endotoxin, are inducers of GM-CSF gene expression, which occurs at least partly by post-transcriptional modification of the GM-CSF mRNA. The biological effects of GM-CSF are mediated through binding to cell surface receptors, which appear to be widely expressed by hematopoietic cells and also by some non-hematopoietic cells, such as endothelial cells. At least two different functional classes of GM-CSF receptor have been identified. The neutrophil GM-CSF receptor exclusively binds GM-CSF, while interleukin-3 competes for binding of GM-CSF to a second class of receptors detected on some leukemic cell lines. Monitoring of GM-CSF has been found relevant in human prostate cancer, poorly healing wounds, thyroid carcinoma, severe mucositis, fungal infections, AIDS, bone marrow transplantation, renal cell carcinoma and prostate cancer, acute lymphoblastic leukemia pulmonary inflammation, hematological malignancies, infection, lung cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for GM-CSF ELISA Kit (NBP1-91277)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ELISA.


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Bioinformatics

Gene Symbol CSF2
Entrez