GM-CSF Antibody (MP122E9)

Recombinant Mouse GM‑CSF (Catalog # 415-ML) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse GM‑CSF (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse GM‑CSF Monoclonal Antibody (Catalog # MAB415). The ND50 is typically 0.03-0.09 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N, ELISA(Cap)
• Clone: MP122E9
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

GM-CSF Antibody (MP122E9) Summary

• Immunogen: Yeast derived recombinant mouse GM-CSF
  Accession # P01587
• Specificity: Detects mouse GM-CSF in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human GM‑CSF.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CSF2
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.03-0.09 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Mouse GM-CSF Biotinylated Antibody (Catalog number BAF415)
  Standard: Recombinant Mouse GM-CSF (Catalog number 415-ML)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GM-CSF Antibody (MP122E9)

• colony stimulating factor 2 (granulocyte-macrophage)
• Colony-stimulating factor
• CSF
• CSF2
• CSF-2
• GMCSF
• GM-CSF
• GMCSFgranulocyte-macrophage colony-stimulating factor
• granulocyte-macrophage colony stimulating factor
• MGC131935
• MGC138897
• Molgramostim
• molgramostin
• Sargramostim

Background

GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3-5). It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core of four bundled alpha ‑helices (8-10). Mature mouse GM-CSF shares 49-54% amino acid sequence identity with canine, feline, human, and porcine GM-CSF and 69% with rat GM-CSF. GM‑CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5 (11, 12). In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF R alpha (13). The activity of GM-CSF is species specific between human and mouse. Mouse GM-CSF is only weakly active on rat cells, although rat GM-CSF is fully active on mouse cells (14, 15).

1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
2. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:509.
3. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
4. Cao, Y. (2007) J. Clin. Invest. 117:2362.
5. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
6. Heuser, M. et al. (2007) Semin. Hematol. 44:148.
7. Hege, K.M. et al. (2006) Int. Rev. Immunol. 25:321.
8. Kaushansky, K. et al. (1992) Biochemistry 31:1881.
9. Diederichs, K. et al. (1991) Science 254:1779.
10. Gough, N.M. et al. (1984) Nature 309:763.
11. Onetto-Pothier, N. et al. (1990) Blood 75:59.
12. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9655.
13. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
14. Oaks, M.K. et al. (1995) J. Interferon Cytokine Res. 15:1095.
15. Vandenabeele, P. et al. (1990) Lymphokine Res. 9:381.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GM-CSF Antibody (MAB415)(12)

Publications using MAB415

• J Yan, Q Zhao, K Gabrusiewi, LY Kong, X Xia, J Wang, M Ott, J Xu, RE Davis, L Huo, G Rao, SC Sun, SS Watowich, AB Heimberger, S Li FGL2 promotes tumor progression in the CNS by suppressing CD103+ dendritic cell differentiation Nat Commun, 2019;10(1):448. 2019 [PMID: 30683885] (Neutralization, Mouse)
• Eóin N McNamee Targeting GM-CSF in epithelial and vascular remodeling in experimental Eosinophilic Esophagitis Allergy, 2016;0(0):. 2016 [PMID: 27926989] (In Vivo, Mouse)
• Type I interferons and microbial metabolites of tryptophan modulate astrocyte activity and central nervous system inflammation via the aryl hydrocarbon receptor Nat Med, 2016;0(0):. 2016 [PMID: 27158906] (Neutralization, Mouse)
• Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum. J Biol Chem, 2015;290(37):22338-51. 2015 [PMID: 26183780] (Neutralization, Mouse)
• Tanabe , Naoya, Hoshino , Yuma, Marumo , Satoshi, Kiyokawa , Hirofumi, Sato , Susumu, Kinose , Daisuke, Uno , Kazuko, Muro , Shigeo, Hirai , Toyohiro, Yodoi , Junji, Mishima , Michiaki Thioredoxin-1 protects against neutrophilic inflammation and emphysema progression in a mouse model of chronic obstructive pulmonary disease exacerbation. PLoS ONE, 2013;8(11):e79016. 2013 [PMID: 24244404] (Neutralization, Mouse)
• Petrackova M, Stanek L, Mandys V, Dundr P, Vonka V Properties of bcr-abl-transformed mouse 12B1 cells secreting interleukin-2 and granulocyte-macrophage colony stimulating factor (GM-CSF): II. Adverse effects of GM-CSF. Int. J. Oncol., 2012;40(6):1915-22. 2012 [PMID: 22446515] (In Vivo, Mouse)
• Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012;58(2):169-77. 2012 [PMID: 22277799] (Luminex Development, Mouse)
• Cheung R, Shen F, Phillips JH, McGeachy MJ, Cua DJ, Heyworth PG, Pierce RH Activation of MDL-1 (CLEC5A) on immature myeloid cells triggers lethal shock in mice. J. Clin. Invest., 2011;121(11):4446-61. 2011 [PMID: 22005300] (Neutralization, Mouse)
• Wengner AM, Pitchford SC, Furze RC, Rankin SM The coordinated action of G-CSF and ELR + CXC chemokines in neutrophil mobilization during acute inflammation. Blood, 2007;111(1):42-9. 2007 [PMID: 17928531] (Neutralization, Mouse)
• Dumas C, Muyombwe A, Roy G, Matte C, Ouellette M, Olivier M, Papadopoulou B Recombinant Leishmania major secreting biologically active granulocyte-macrophage colony-stimulating factor survives poorly in macrophages in vitro and delays disease development in mice. Infect. Immun., 2003;71(11):6499-509. 2003 [PMID: 14573672] (ICC, Mouse)
• Pechova J, StÄ•pánová G, Kovar L, Kopecky J Tick salivary gland extract-activated transmission of Borrelia afzelii spirochaetes. Folia Parasitol., 2002;49(2):153-9. 2002 [PMID: 12194488] (ELISA Development, Mouse)
• Tomaki M, Zhao LL, Sjostrand M, Linden A, Ichinose M, Lotvall J Comparison of effects of anti-IL-3, IL-5 and GM-CSF treatments on eosinophilopoiesis and airway eosinophilia induced by allergen. Pulm Pharmacol Ther, 2002;15(2):161-8. 2002 [PMID: 12090790] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: CSF2
• Uniprot:
  • P01587()