GM-CSF Antibody (MP122E9) [Unconjugated]

Recombinant Mouse GM‑CSF (Catalog # 415-ML) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse GM‑CSF (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse GM‑CSF Monoclonal Antibody (Catalog # MAB415). The ND50 is typically 0.03-0.09 µg/mL.

Effects of TRX on inflammatory cytokines in BALF from exposed to cigarette smoke and challenged with poly(I:C).(A) KC and (B) GM-CSF in BALF obtained 6 h and 3 days after poly(I:C) challenge. (C) Messenger RNA of GM-CSF in lung homogenates 3 days after poly(I:C) challenge in mice exposed to cigarette smoke treated with or without TRX. (D) Neutrophil and lymphocyte counts in BALF 3 days after poly(I:C) challenge in mice exposed to cigarette smoke treated with and without TRX or anti-GM-CSF antibody. Error bars represent standard deviation (SD) (A, B, and C, n = 3–4 per group; D and E, n = 5 per group); *p<0.05. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0079016), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, B/N, ELISA(Cap)
• Clone: MP122E9
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

GM-CSF Antibody (MP122E9) [Unconjugated] Summary

• Immunogen: Yeast derived recombinant mouse GM-CSF
  Accession # P01587
• Specificity: Detects mouse GM-CSF in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human GM‑CSF.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CSF2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.03-0.09 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Mouse GM-CSF Biotinylated Antibody (Catalog number BAF415)
  Standard: Recombinant Mouse GM-CSF (Catalog number 415-ML)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GM-CSF Antibody (MP122E9) [Unconjugated]

• colony stimulating factor 2 (granulocyte-macrophage)
• Colony-stimulating factor
• CSF
• CSF2
• CSF-2
• GMCSF
• GM-CSF
• GMCSFgranulocyte-macrophage colony-stimulating factor
• granulocyte-macrophage colony stimulating factor
• MGC131935
• MGC138897
• Molgramostim
• molgramostin
• Sargramostim

Background

GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3-5). It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core of four bundled alpha ‑helices (8-10). Mature mouse GM-CSF shares 49-54% amino acid sequence identity with canine, feline, human, and porcine GM-CSF and 69% with rat GM-CSF. GM‑CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5 (11, 12). In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF R alpha (13). The activity of GM-CSF is species specific between human and mouse. Mouse GM-CSF is only weakly active on rat cells, although rat GM-CSF is fully active on mouse cells (14, 15).

1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
2. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:509.
3. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
4. Cao, Y. (2007) J. Clin. Invest. 117:2362.
5. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
6. Heuser, M. et al. (2007) Semin. Hematol. 44:148.
7. Hege, K.M. et al. (2006) Int. Rev. Immunol. 25:321.
8. Kaushansky, K. et al. (1992) Biochemistry 31:1881.
9. Diederichs, K. et al. (1991) Science 254:1779.
10. Gough, N.M. et al. (1984) Nature 309:763.
11. Onetto-Pothier, N. et al. (1990) Blood 75:59.
12. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9655.
13. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
14. Oaks, M.K. et al. (1995) J. Interferon Cytokine Res. 15:1095.
15. Vandenabeele, P. et al. (1990) Lymphokine Res. 9:381.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GM-CSF Antibody (MAB415)(18)

Publications using MAB415

• Hillers-Ziemer LE, Williams AE, Janquart A et al. Obesity-Activated Lung Stromal Cells Promote Myeloid Lineage Cell Accumulation and Breast Cancer Metastasis Cancers (Basel) 2021-02-28 [PMID: 33670906]
• Hillers-Ziemer LE, Williams AE, Janquart A et al. Obesity-Activated Lung Stromal Cells Promote Myeloid Lineage Cell Accumulation and Breast Cancer Metastasis Cancers (Basel) 2/28/2021 [PMID: 33670906]
• Sonia Iyer, Shuang Zhang, Simge Yucel, Heiko Horn, Sean G. Smith, Ferenc Reinhardt et al. Genetically defined syngeneic mouse models of ovarian cancer as tools for the discovery of combination immunotherapy. Cancer Discovery 2021-02-01 [PMID: 33158843]
• Christa I. DeVette, Harika Gundlapalli, Shu-Chin Alicia Lai, Curtis P. McMurtrey, Ashley R. Hoover, Hem R. Gurung et al. A pipeline for identification and validation of tumor-specific antigens in a mouse model of metastatic breast cancer OncoImmunology 2020-01-01 [PMID: 32002300]
• Ranveig Braathen, Heidi C. L. Spång, Mona M. Lindeberg, Even Fossum, Gunnveig Grødeland, Agnete B. Fredriksen et al. The Magnitude and IgG Subclass of Antibodies Elicited by Targeted DNA Vaccines Are Influenced by Specificity for APC Surface Molecules ImmunoHorizons 2018-01-01 [PMID: 31022690]
• J Yan, Q Zhao, K Gabrusiewi, LY Kong, X Xia, J Wang, M Ott, J Xu, RE Davis, L Huo, G Rao, SC Sun, SS Watowich, AB Heimberger, S Li FGL2 promotes tumor progression in the CNS by suppressing CD103+ dendritic cell differentiation Nat Commun, 2019-01-25;10(1):448. 2019-01-25 [PMID: 30683885] (Neutralization, Mouse)
• Maria Casanova-Acebes, José A. Nicolás-Ávila, Jackson LiangYao Li, Susana García-Silva, Akhila Balachander, Andrea Rubio-Ponce et al. Neutrophils instruct homeostatic and pathological states in naive tissues Journal of Experimental Medicine 2018-11-05 [PMID: 30282719] (Neutralization, Transgenic Mouse)
• Eóin N McNamee Targeting GM-CSF in epithelial and vascular remodeling in experimental Eosinophilic Esophagitis Allergy, 2017-01-23;0(0):. 2017-01-23 [PMID: 27926989] (In Vivo, Mouse)
• Type I interferons and microbial metabolites of tryptophan modulate astrocyte activity and central nervous system inflammation via the aryl hydrocarbon receptor Nat Med, 2016-05-09;0(0):. 2016-05-09 [PMID: 27158906] (Neutralization, Mouse)
• Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum. J Biol Chem, 2015-07-16;290(37):22338-51. 2015-07-16 [PMID: 26183780] (Neutralization, Mouse)
• Tanabe , Naoya, Hoshino , Yuma, Marumo , Satoshi, Kiyokawa , Hirofumi, Sato , Susumu, Kinose , Daisuke, Uno , Kazuko, Muro , Shigeo, Hirai , Toyohiro, Yodoi , Junji, Mishima , Michiaki Thioredoxin-1 protects against neutrophilic inflammation and emphysema progression in a mouse model of chronic obstructive pulmonary disease exacerbation. PLoS ONE, 2013-11-11;8(11):e79016. 2013-11-11 [PMID: 24244404] (Neutralization, Mouse)
• Petrackova M, Stanek L, Mandys V, Dundr P, Vonka V Properties of bcr-abl-transformed mouse 12B1 cells secreting interleukin-2 and granulocyte-macrophage colony stimulating factor (GM-CSF): II. Adverse effects of GM-CSF. Int. J. Oncol., 2012-03-22;40(6):1915-22. 2012-03-22 [PMID: 22446515] (In Vivo, Mouse)
• Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-01-25;58(2):169-77. 2012-01-25 [PMID: 22277799] (Luminex Development, Mouse)
• Cheung R, Shen F, Phillips JH, McGeachy MJ, Cua DJ, Heyworth PG, Pierce RH Activation of MDL-1 (CLEC5A) on immature myeloid cells triggers lethal shock in mice. J. Clin. Invest., 2011-10-17;121(11):4446-61. 2011-10-17 [PMID: 22005300] (Neutralization, Mouse)
• Wengner AM, Pitchford SC, Furze RC, Rankin SM The coordinated action of G-CSF and ELR + CXC chemokines in neutrophil mobilization during acute inflammation. Blood, 2007-10-10;111(1):42-9. 2007-10-10 [PMID: 17928531] (Neutralization, Mouse)
• Dumas C, Muyombwe A, Roy G, Matte C, Ouellette M, Olivier M, Papadopoulou B Recombinant Leishmania major secreting biologically active granulocyte-macrophage colony-stimulating factor survives poorly in macrophages in vitro and delays disease development in mice. Infect. Immun., 2003-11-01;71(11):6499-509. 2003-11-01 [PMID: 14573672] (ICC, Mouse)
• Pechova J, StÄ•pánová G, Kovar L, Kopecky J Tick salivary gland extract-activated transmission of Borrelia afzelii spirochaetes. Folia Parasitol., 2002-01-01;49(2):153-9. 2002-01-01 [PMID: 12194488] (ELISA Development, Mouse)
• Tomaki M, Zhao LL, Sjostrand M, Linden A, Ichinose M, Lotvall J Comparison of effects of anti-IL-3, IL-5 and GM-CSF treatments on eosinophilopoiesis and airway eosinophilia induced by allergen. Pulm Pharmacol Ther, 2002-01-01;15(2):161-8. 2002-01-01 [PMID: 12090790] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: CSF2
• Uniprot:
  • P01587()