GM-CSF Antibody (6804) [Unconjugated]

210-TA), and Recombinant Human TGF-beta 1 (240-B) were stained with Mouse Anti-Human GM-CSF Monoclonal Antibody (Catalog # MAB615, filled histogram) or isotype control antibody (IC002P, open histogram) followed by Goat anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol." > 210-TA), and Recombinant Human TGF-beta 1 (240-B) were stained with Mouse Anti-Human GM-CSF Monoclonal Antibody (Catalog # MAB615, filled histogram) or isotype control antibody (IC002P, open histogram) followed by Goat anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol." title="Human dendritic cells treated with LPS, Recombinant Human TNF-a (210-TA), and Recombinant Human TGF-beta 1 (240-B) were stained with Mouse Anti-Human GM-CSF Monoclonal Antibody (Catalog # MAB615, filled histogram) or isotype control antibody (IC002P, open histogram) followed by Goat anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol." />

Human dendritic cells treated with LPS, Recombinant Human TNF-a (<a class=

Kinetics of Azithromycin Effects on NALP3 Protein in THP-1 Monocytes. a THP-1 cells were treated with azithromycin 50ug/ml for the times shown prior to immunoblot analysis for indicated proteins. b THP-1 cells were treated with LPS 500 ng/ml for times shown prior to analysis. c THP-1 cells were treated with azithromycin (50ug/ml) and LPS (500 ng/ml) for times shown prior to immunoblot analysis. The data representative of 2–3 separate experiments Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28659178), licensed under a CC-BY license. Not internally tested by R&D Systems.

Kinetics of Azithromycin Effects on NALP3 Protein in THP-1 Monocytes. a THP-1 cells were treated with azithromycin 50ug/ml for the times shown prior to immunoblot analysis for indicated proteins. b THP-1 cells were treated with LPS 500 ng/ml for times shown prior to analysis. c THP-1 cells were treated with azithromycin (50ug/ml) and LPS (500 ng/ml) for times shown prior to immunoblot analysis. The data representative of 2–3 separate experiments Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28659178), licensed under a CC-BY license. Not internally tested by R&D Systems.

Kinetics of Azithromycin Effects on NALP3 Protein in THP-1 Monocytes. a THP-1 cells were treated with azithromycin 50ug/ml for the times shown prior to immunoblot analysis for indicated proteins. b THP-1 cells were treated with LPS 500 ng/ml for times shown prior to analysis. c THP-1 cells were treated with azithromycin (50ug/ml) and LPS (500 ng/ml) for times shown prior to immunoblot analysis. The data representative of 2–3 separate experiments Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28659178), licensed under a CC-BY license. Not internally tested by R&D Systems.

215-GM) was serially diluted and captured by Mouse Anti-Human GM‑CSF Monoclonal Antibody (Catalog # MAB615) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human GM‑CSF Monoclonal Antibody (Catalog # MAB215) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" > 215-GM) was serially diluted and captured by Mouse Anti-Human GM‑CSF Monoclonal Antibody (Catalog # MAB615) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human GM‑CSF Monoclonal Antibody (Catalog # MAB215) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" title="Recombinant Human GM‑CSF (Catalog # 215-GM) was serially diluted and captured by Mouse Anti-Human GM‑CSF Monoclonal Antibody (Catalog # MAB615) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human GM‑CSF Monoclonal Antibody (Catalog # MAB215) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" />

Recombinant Human GM‑CSF (Catalog # <a class=

• Reactivity: Hu
• Applications: CyTOF-ready, ELISA(Cap), Flow
• Clone: 6804
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GM-CSF Antibody (6804) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human GM-CSF
  Ala18-Glu144
  Accession # P04141
• Specificity: Detects human GM-CSF in ELISAs. In ELISAs, no cross-reactivity with recombinant mouse GM‑CSF, recombinant rat GM‑CSF, recombinant human (rh) M‑CSF, rhG‑CSF, rhIL‑5‑R alpha , or rhIL‑3 R alpha is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSF2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
• Application Notes: ELISA Detection: Human GM-CSF Biotinylated Antibody (Catalog number BAM215)
  Standard: Recombinant Human GM-CSF (Catalog number 215-GM)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GM-CSF Antibody (6804) [Unconjugated]

• colony stimulating factor 2 (granulocyte-macrophage)
• Colony-stimulating factor
• CSF
• CSF2
• CSF-2
• GMCSF
• GM-CSF
• GMCSFgranulocyte-macrophage colony-stimulating factor
• granulocyte-macrophage colony stimulating factor
• MGC131935
• MGC138897
• Molgramostim
• molgramostin
• Sargramostim

Background

GM-CSF is a growth factor produced by a variety of lymphoid cells. It binds to receptor heterodimers consisting of a GM-CSF-specific alpha chain and the common beta  chain that is shared by the high affinity receptors for IL-3 and IL-5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GM-CSF Antibody (MAB615)(9)

Publications using MAB615

• Girija Goyal, Pranav Prabhala, Gautam Mahajan, Bruce Bausk, Tal Gilboa, Liangxia Xie et al. Ectopic Lymphoid Follicle Formation and Human Seasonal Influenza Vaccination Responses Recapitulated in an Organ‐on‐a‐Chip Advanced Science 2022-05-01 [PMID: 35289122]
• J Li, A Wuethrich, AAI Sina, HH Cheng, Y Wang, A Behren, PN Mainwaring, M Trau A digital single-molecule nanopillar SERS platform for predicting and monitoring immune toxicities in immunotherapy Nature Communications, 2021-02-17;12(1):1087. 2021-02-17 [PMID: 33597530] (ELISA Development, Human)
• Valentin Haug, Branislav Kollar, Sotirios Tasigiorgos, Yori Endo, Martin Kauke, Ali-Farid Safi et al. Hypothermic Ex Situ Perfusion of Human Limbs With Acellular Solution for 24 Hours Transplantation 2020-09-01 [PMID: 32168040]
• EA Lendermon, TA Coon, JS Bednash, NM Weathingto, JF McDyer, RK Mallampall Azithromycin decreases NALP3 mRNA stability in monocytes to limit inflammasome-dependent inflammation Respir. Res., 2017-06-28;18(1):131. 2017-06-28 [PMID: 28659178] (Western Blot, Human)
• Rosen L, Freeman A, Yang L, Jutivorakool K, Olivier K, Angkasekwinai N, Suputtamongkol Y, Bennett J, Pyrgos V, Williamson P, Ding L, Holland S, Browne S Anti-GM-CSF autoantibodies in patients with cryptococcal meningitis. J Immunol, 2013-03-18;190(8):3959-66. 2013-03-18 [PMID: 23509356] (Flow Cytometry, Human)
• Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E A multiplex immunoassay for human adipokine profiling. Clin. Chem., 2010-06-08;56(0):1320. 2010-06-08 [PMID: 20530731] (Luminex Development, Human)
• Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62. 2008-03-01 [PMID: 18327985] (Luminex Development, Human)
• Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Blood, 2007-02-08;109(10):4272-9. 2007-02-08 [PMID: 17289812] (Luminex Development, Human)
• Hirst SJ, Hallsworth MP, Peng Q Selective induction of eotaxin release by interleukin-13 or interleukin-4 in human airway smooth muscle cells is synergistic with interleukin-1beta and is mediated by the interleukin-4 receptor alpha-chain. Am. J. Respir. Crit. Care Med., 2002-04-15;165(8):1161-71. 2002-04-15 [PMID: 11956062] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: CSF2
• Uniprot:
  • P04141(Human)