| Reactivity | HuSpecies Glossary |
| Applications | Flow |
| Clone | 6804 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Alexa Fluor 350 |
| Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | IC615V-100UG | |||
| Alexa Fluor 488 | IC615G-100UG | |||
| Alexa Fluor 594 | IC615T-100UG | |||
| Alexa Fluor 647 | IC615R-100UG | |||
| Alexa Fluor 700 | IC615N-100UG | |||
| Alexa Fluor 750 | IC615S-100UG | |||
| Phycoerythrin | IC615P | |||
| Unconjugated | MAB615-100 | |||
| Immunogen | E. coli-derived recombinant human GM-CSF Ala18-Glu144 Accession # P04141 |
| Specificity | Detects human GM-CSF in ELISAs. In ELISAs, no cross-reactivity with recombinant mouse GM‑CSF, recombinant rat GM‑CSF, recombinant human (rh) M‑CSF, rhG‑CSF, rhIL‑5‑R alpha, or rhIL‑3 R alpha is observed. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CSF2 |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells. |
| Storage | Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
| Buffer | Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. |
| Preservative | 0.09% Sodium Azide |
| Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
![GM-CSF Antibody (6804) [Alexa Fluor® 350]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "GM-CSF Antibody (6804) [Alexa Fluor® 350]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | IC615U | |
| Alexa Fluor 405 | IC615V | |
| Alexa Fluor 488 | IC615G | |
| Alexa Fluor 594 | IC615T | |
| Alexa Fluor 647 | IC615R | |
| Alexa Fluor 700 | IC615N | |
| Alexa Fluor 750 | IC615S | |
| Phycoerythrin | IC615P |
Diseases for GM-CSF Antibody (IC615U)Discover more about diseases related to GM-CSF Antibody (IC615U).
| Pathways for GM-CSF Antibody (IC615U)View related products by pathway.
|
PTMs for GM-CSF Antibody (IC615U)Learn more about PTMs related to GM-CSF Antibody (IC615U).
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CSF2 |
![Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.](http://images.novusbio.com/images2/Albumin-Antibody-Western-Blot-NB600-41532-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Albumin Antibody [NB600-41532] - NLRP12 downregulates multiple JNK activating pathways in hepatocytes. For checking the purity of primary hepatocytes, hepatocytes isolated from healthy WT and Nlrp12-/- mouse livers were immunostained with antibody for hepatocyte-specific marker Albumin and counterstained with DAPI. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/40396), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Albumin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-41532-img0003.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0018.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/images2/HLA DQ Antibody (SPV-L3)-Flow Cytometry-NBP2-45041-img0001.jpg)
![Western Blot: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of rat whole brain lysates using mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain. Strong bands at 40, 45kDa correspond to DCX protein, detected only in the developing brain.](http://images.novusbio.com/images2/Doublecortin-Antibody-(3E1)-Western-Blot-NBP1-92684-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in red, and costained with chicken pAb to Microtubule Associated Protein2 (MAP2), dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. DCX antibody reveals strong cytoplasmic staining in a population of small developing neurons and their processes, while MAP2 antibody stains dendrites and perikarya of mature neurons. Therefore DCX antibody is an excellent marker of developing neuronal cells.](http://images.novusbio.com/images2/Doublecortin-Antibody-(3E1)-Immunocytochemistry-Immunofluorescence-NBP1-92684-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Rat brain neural cultures stained with NBP1-92684 (green), a chicken polyclonal antibody to GFAP (NB300-213, red) and DNA (blue). The NBP1-92684 antibody reveals strong cytoplasmic staining in a population of small developing neurons. These cells are often found in small clumps as in these cultures, as in this example. Note that they are not positive for MAP2, which is characteristic of more mature neurons, and that the mature neuron shown is negative for NBP1-92684. This Doublecortin antibody is therefore an excellent marker of developing neuronal cells.](http://images.novusbio.com/images2/Doublecortin-Antibody-(3E1)-Immunocytochemistry-Immunofluorescence-NBP1-92684-img0003.jpg)