GM-CSF Antibody (30-4)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Func, IHC, IHC-Fr, IHC-P
Clone
30-4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

GM-CSF Antibody (30-4) Summary

Immunogen
Recombinant protein corresponding to amino acids 14 to 144 of human GM-CSF produced in E. coli.
Specificity
NB100-65022 recognizes recombinant and native human granulocyte macrophage colony stimulating factor (GM-CSF). Human Granulocyte colony stimulating factor (GM-CSF) is a 14.6kDa glycoprotein, which is produced by T cells, macrophages, fibroblasts and endothelial cells. GM-CSF is involved in the stimulation of proliferation and differentiation of granulocyte and macrophage progenitor cells. The neutralisation dose (ND50) of this batch, as determined by the proliferation of TF-1 cells, is approximately 0.3-0.5 ug/ml in the presence of 0.5 ng/ml of recombinant human GM-CSF. The ND50 may vary depending on conditions.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CSF2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA 0.5-1 ug/ml
  • Functional 0.3-0.5 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 8-25 ug/ml
  • Immunohistochemistry-Paraffin
Application Notes
In WB should be used under non-reducing conditions, reducing conditions are not recommended.Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24737547)
Positive Control
GM-CSF Lysate (NBL1-09515)
Publications
Read Publication using
NB100-65022 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 1.0% Trehalose
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for GM-CSF Antibody (30-4)

  • colony stimulating factor 2 (granulocyte-macrophage)
  • Colony-stimulating factor
  • CSF
  • CSF2
  • GMCSF
  • GM-CSF
  • GMCSFgranulocyte-macrophage colony-stimulating factor
  • granulocyte-macrophage colony stimulating factor
  • MGC131935
  • MGC138897
  • molgramostin
  • sargramostim

Background

The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GM-CSF Antibody (NB100-65022)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(1)
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Human
(1)
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Product General Protocols

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CSF2
Entrez
Uniprot