GM-CSF Antibody (30-4)

Product Discontinued

GM-CSF Antibody (30-4) Summary

• Immunogen: Recombinant protein corresponding to amino acids 14 to 144 of human GM-CSF produced in E. coli.
• Specificity: NB100-65022 recognizes recombinant and native human granulocyte macrophage colony stimulating factor (GM-CSF). Human Granulocyte colony stimulating factor (GM-CSF) is a 14.6kDa glycoprotein, which is produced by T cells, macrophages, fibroblasts and endothelial cells. GM-CSF is involved in the stimulation of proliferation and differentiation of granulocyte and macrophage progenitor cells. The neutralisation dose (ND50) of this batch, as determined by the proliferation of TF-1 cells, is approximately 0.3-0.5 ug/ml in the presence of 0.5 ng/ml of recombinant human GM-CSF. The ND50 may vary depending on conditions.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSF2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 0.5-1 ug/ml
  • Functional 0.3-0.5 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 8-25 ug/ml
  • Immunohistochemistry-Paraffin
  • Western Blot 1-2 ug/ml
• Application Notes: In WB should be used under non-reducing conditions, reducing conditions are not recommended.Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24737547)
• Control:

  GM-CSF Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 1% Trehalose
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for GM-CSF Antibody (30-4)

• colony stimulating factor 2 (granulocyte-macrophage)
• Colony-stimulating factor
• CSF
• CSF2
• CSF-2
• GMCSF
• GM-CSF
• GMCSFgranulocyte-macrophage colony-stimulating factor
• granulocyte-macrophage colony stimulating factor
• MGC131935
• MGC138897
• Molgramostim
• molgramostin
• Sargramostim

Background

The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GM-CSF Antibody (NB100-65022)(1)

Publication using NB100-65022

• Nebiker C, Han J, Castori-Eppenberger S et al. GM-CSF production by tumor cells is associated with improved survival in colorectal cancer. Clin. Cancer Res. 2014-04-15 [PMID: 24737547] (IHC-P, Human)

Bioinformatics

• Gene Symbol: CSF2
• Entrez:
  • 1437(Human)
  • 12981(Mouse)
• Uniprot:
  • P04141(Human)