Glypican 1 Antibody - BSA Free

Western Blot: Glypican 1 Antibody [NBP3-13127] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Glypican 1 antibody (NBP3-13127) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Glypican 1 Antibody [NBP3-13127] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody (NBP3-13127) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Glypican 1 Antibody [NBP3-13127] - Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody (NBP3-13127) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Glypican 1 antibody detects Glypican 1 protein at cell membrane in human pancreas by immunohistochemical analysis. Sample: Paraffin-embedded human pancreas, normal (left) and cancer (right). Glypican 1 antibody (NBP3-13127) diluted at 1:250. br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Rt, RM
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Glypican 1 Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Glypican 1. The exact sequence is proprietary.
• Predicted Species: Rat (90%), Rhesus Macaque (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPC1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 62 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (89%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Glypican 1 Antibody - BSA Free

• FLJ38078
• Glypican 1
• glypican proteoglycan 1
• glypican
• glypican-1
• GPC1

Background

GPC1(Glypican 1) is a cell surface proteoglycan that contains heparan sulphate. The protein is attached to the cell membrane by a GPI anchor. GPC1 is required for efficient TGF-beta1 signaling in pancreatic cancer cells. Members of the glypican-related integral membrane proteoglycan family (GRIPS) may play a role in the control of cell division and growth regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPC1