Glyoxalase I Antibody (Glo1a) - Azide and BSA Free

Western Blot: Glyoxalase I Antibody (Glo1a) - Azide and BSA Free [NBP2-80755] - HeLa whole cell extracts. Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: Glyoxalase I Antibody (Glo1a) - Azide and BSA Free [NBP2-80755] - Immunostaining of human retinal endothelial cells. Immunoreactivity for hGlx-1 was detected in human retinal endothelial cells stained with Glo1a mAb using anti mouse IgG Texas Red as secondary Ab. The cells were counter stained with phalloidin (green) and DAPI (blue), nuclear stain. No reactivity was detected in control cells, stained without Go1a mAb. Figure is a representative of 3 independent experiments. Photo courtesy of Dr. Nagaraj, Case Western Reserve University. Image from the standard format of this antibody.

Western Blot: Glyoxalase I Antibody (Glo1a) - Azide and BSA Free [NBP2-80755] - Detection of GLO-1 in human cell lines. Lane 1,2 and 3: MG-63 osteosarcoma cell line (positive control), 402-91 liposarcoma cell line and 402-91 liposarcoma cell line resistant to trabectedine. This image was submitted via customer Review. Image from the standard format of this antibody.

Simple Western: Glyoxalase I Antibody (Glo1a) - Azide and BSA Free [NBP2-80755] - Simple Western lane view shows a specific band for GLO1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from the standard format of this antibody.

Simple Western: Glyoxalase I Antibody (Glo1a) - Azide and BSA Free [NBP2-80755] - Simple Western lane view shows a specific band detected for GLO1 in HeLa lysate. This experiment was performed under reducing conditions using the Wes or Sally Sue separation system 12-230kDa (or 66-440kDa). Image from the standard format of this antibody.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ELISA, ICC/IF, IP
• Clone: Glo1a
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Glyoxalase I Antibody (Glo1a) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Glyoxalase I Antibody (Glo1a) - Azide and BSA Free (NBP1-19015) is a monoclonal antibody validated for use in WB, ELISA, ICC/IF, Simple Western and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human GLO1 protein [Swiss-Prot #Q04760].
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GLO1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 0.15 ng / 1 ug protein
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunoprecipitation reported in scientific literature
  • Simple Western 1:50
  • Western Blot
• Application Notes: In Western blot, a band is seen approx. 21 kDa.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:50. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine, 0.15 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Glyoxalase I Antibody (Glo1a) - Azide and BSA Free

• Aldoketomutase
• EC 4.4.1.5
• GLO1
• GLOD1
• Glx I
• GLYI
• glyoxalase domain containing 1
• Glyoxalase I
• glyoxalase Ialdoketomutase
• Ketone-aldehyde mutase
• lactoyl glutathione lyase
• lactoylglutathione lyase
• Methylglyoxalase
• S-D-lactoylglutathione methylglyoxal lyase

Background

Glyoxalase 1 (GLO1) is the main enzyme in glyoxalase system, a metabolic pathway responsible for detoxifying alpha-oxoaldehydes, particularly methylglyoxal; and is known for converting reactive dicarbonyls into non-toxic intermediates ultimately protecting living cells from producing and accumulating advanced glycation end products, AGEs. GLO1 has also been studied with reference to anxiety, and a murine model showed that this gene's overexpression increases anxiety-related behavior by reducing the GABAA receptor agonist MG. Moreover, an association analysis in a human population with anxiety disorder subtypes suggested a role for GLO1's implication in this phenotype. GLO1 plays an important role in tumour growth and partly in chemotherapy survival as evidenced by a number of studies suggesting that some tumours rely on GLO1 to increase their viability and resistance to multiple chemotherapeutic agents. Furthermore, genetic studies have implicated GLO1 polymorphisms in panic disorder and restless legs syndrome (RLS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GLO1