Glutamine Synthetase Antibody (GLUL/8996R)

Formalin-fixed, paraffin-embedded human brain stained with Glutamine Synthetase antibody (GLUL/8996R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human brain stained with Glutamine Synthetase antibody (GLUL/8996R). Inset: PBS instead of primary antibody; secondary only negative control.

• Reactivity: Hu
• Applications: IHC
• Clone: GLUL/8996R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Glutamine Synthetase Antibody (GLUL/8996R) Summary

• Description: Store at 2 to 8C. Non-hazardous. No MSDS required.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment (around aa 200-373) of human Glutamine Synthetase protein (exact sequence is proprietary)
• Localization: Cytoplasm. Mitochondrion.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GLUL
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2ug/ml
• Application Notes: Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Glutamine Synthetase Antibody (GLUL/8996R)

• cell proliferation-inducing protein 59
• EC 4.1.1.15
• EC 6.3.1.2
• GLNSPIG59
• Glutamate decarboxylase
• glutamate-ammonia ligase (glutamine synthase)
• glutamate-ammonia ligase
• Glutamate--ammonia ligase
• glutamine synthase
• glutamine synthetase
• GS
• PIG43
• proliferation-inducing protein 43

Background

Glutamine synthetase is part of the glutamine synthetase family. Specifically, Glutamine synthetase catalyzes the condensation of glutamate and ammonia to form glutamine, during the metabolism of nitrogen. Ammonia incorporation in animals occurs through the actions of glutamate dehydrogenase and glutamine synthase. Glutamate plays the central role in mammalian nitrogen flow, serving as both a nitrogen donor and nitrogen acceptor.

Glutamine synthetase antibodies are useful tools for studying nitrogen metabolism and regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GLUL