Glutamate Receptor 3 Antibody (1059821) [Unconjugated]

GluR3 was detected in immersion fixed TT human medullary thyroid cancer cell line (positive) and absent in THP‑1 human acute monocytic leukemia cell line (negative) using Mouse Anti-Human GluR3 Monoclonal Antibody (Catalog # MAB11329) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 1059821
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Glutamate Receptor 3 Antibody (1059821) [Unconjugated] Summary

• Immunogen: Synthetic Peptide
  Accession # P42263
• Specificity: Detects human GluR3 in direct ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glutamate Receptor 3 Antibody (1059821) [Unconjugated]

• AMPA 3
• AMPA3
• AMPA-selective glutamate receptor 3
• GluA3
• GluR3
• GluR-3
• GLURC
• GLUR-C
• GLUR-K3
• glutamate receptor C
• glutamate receptor subunit 3
• glutamate receptor, ionotrophic, AMPA 3
• GRIA3

Background

GluR3 is a receptor for glutamate. Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiological processes. This gene belongs to a family of AMPA receptors.  GluR3 is a gene product of the GRIA3 gene and its pre-mRNA is subject to RNA editing. In humans, 80-90% of GRIA3 transcripts are edited. This allows sustained response to rapid stimuli. There is a tentative link between defective GRIA3 variants and a highly elevated risk of schizophrenia.

1. Entrez Gene: GRIA3 glutamate receptor, ionotropic, AMPA 3.
2. Seeburg PH, Higuchi M, Sprengel R. "RNA Editing of Brain Glutamate Receptor channels:  Mechanism and Physiology". Brain Res. Brain Res. Rev. 1998, May; 26(2-3):217.
3. Lomeli H, Mosbacher J, Höger T, Geiner JR, Kuner T, Monyer H, Higuchi M, Bach A, Seeburg PH., "Control of Kinetic Properties of AMPA rREceptor Channels by Nuclear RNA Editing". Science. 1994, Dec; 266(5191):1709.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P42263(Human)