Glutamate Dehydrogenase Antibody (SR1881) - BSA Free

Western Blot: Glutamate Dehydrogenase Antibody (SR1881) [NBP3-22240] - Western blot analysis using NBP3-22240 in HepG2 cell lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: SR1881
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Glutamate Dehydrogenase Antibody (SR1881) - BSA Free Summary

• Description: Novus Biologicals Rabbit Glutamate Dehydrogenase Antibody (SR1881) - BSA Free (NBP3-22240) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human Glutamate Dehydrogenase (Uniprot #: P00367)
• Specificity: Detects endogenous levels of total Glutamate Dehydrogenase
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GLUD1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:500
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Glutamate Dehydrogenase Antibody (SR1881) - BSA Free

• EC 1.4.1
• EC 1.4.1.3
• GDH 1
• GDH
• GDH1
• GLUD
• glutamate dehydrogenase (NAD(P)+)
• Glutamate Dehydrogenase 1
• glutamate dehydrogenase 1, mitochondrial
• MGC132003

Background

One enzyme central to the metabolism of glutamate is glutamate dehydrogenase (GDH1; EC 1.4.1.3), that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex. It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GLUD1