GluR2 Antibody (JU20-43)

Western Blot: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of GluR2 on rat brain lysates with Rabbit anti-GluR2 antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 99 kDa Observed band size: 90 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: GluR2 Antibody (JU20-43) [NBP2-75510] - Staining GluR2 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GluR2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/Immunofluorescence: GluR2 Antibody (JU20-43) [NBP2-75510] - Staining GluR2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of paraffin-embedded mouse brain tissue using anti-GluR2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of paraffin-embedded rat brain tissue using anti-GluR2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of paraffin-embedded rat brain tissue with Rabbit anti-GluR2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clone: JU20-43
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

GluR2 Antibody (JU20-43) Summary

• Description: Novus Biologicals Rabbit GluR2 Antibody (JU20-43) (NBP2-75510) is a recombinant monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-GluR2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human GluR2 aa 181-230 / 883. (SwissProt: P42262 Human; SwissProt: P23819 Mouse; SwissProt: P19491 Rat)
• Localization: Cell membrane, postsynaptic cell membrane, postsynaptic density membrane, endoplasmic reticulum membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GRIA2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500
• Theoretical MW: 99 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for GluR2 Antibody (JU20-43)

• AMPA 2
• AMPA2
• GluA2
• GluR2
• GLURB
• GluR-K2
• glutamate receptor, ionotropic, AMPA 2
• GRIA2
• HBGR2

Background

The GRIA2 gene encodes for glutamate receptor 2 with an isoform flop of 883 amino acids, 98 kDA, isoform flip of 883 amino acids and 98 kDA, and an isoform 3 of 901 amino acids long and 100 kDA. Glutamate receptors function as a ligand-gated ion channel in the central nervous system and are the predominant excitatory neurotransmitter receptors in the brain. GRIA2 has been researched regarding its role in Alzheimer's disease, multiple sclerosis, motor neuron disease, epilepsy, anorexia, bioplar disorder, and neurodegeneration. GRIA2 is involved in the CREB pathway, intracellular calcium signaling, glutamic acid signaling, and calcium channels as well as interacts with genes NSF, GRIA3, DLG4, GRID2, and SDCBP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GluR2 Antibody (NBP2-75510)(2)

Publications using NBP2-75510

• Kulikov AA, Naumova AA, Dorofeeva NA et al. Dynamics of neurodegeneration in the hippocampus of Krushinsky-Molodkina rats correlates with the progression of limbic seizures Epilepsy & behavior : E&B 2022-07-15 [PMID: 35849865]
• Zhang N, Zhang Z, He R et al. GLAST-CreER(T2) mediated deletion of GDNF increases brain damage and exacerbates long-term stroke outcomes after focal ischemic stroke in mouse model Glia 2020-06-04 [PMID: 32497340]

Bioinformatics

• Gene Symbol: GRIA2