ADAR Antibody

Immunohistochemistry-Paraffin: ADAR Antibody [NBP1-90242] - Staining in human testis and skeletal muscle tissues using NBP1-90242 antibody. Corresponding ADAR RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: ADAR Antibody [NBP1-90242] - Staining of human cerebellum shows strong nuclear positivity in all layers.

Immunohistochemistry-Paraffin: ADAR Antibody [NBP1-90242] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: ADAR Antibody [NBP1-90242] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: ADAR Antibody [NBP1-90242] - Staining of human testis shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts.

Staining of human cerebellum, endometrium, skeletal muscle and testis using NBP1-90242 (A) shows similar protein distribution across tissues to independent antibody NBP3-05523 (B).

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ADAR Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids:SDNQPEGMISESLDNLESMMPNKVRKIGELVRYLNTNPVGGLLEYARSHGFAAEFKLVDQSGPPHEPKFVYQAKVGGRWFPAVCAHSKKQGKQEAADAALRVLIGENEKAERMGFTEVTPVTGASLRRTMLLLSRSPEA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADAR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: PFA/Triton X-100

Reactivity Notes

Mouse (86%), Rat (85%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ADAR Antibody

• ADAR
• ADAR1
• ADAR1DSH
• adenosine deaminase, RNA-specific
• DRADA
• DRADAP136
• DSRAD
• DSRADadenosine deaminase acting on RNA 1-A
• EC 3.5.4
• EC 3.5.4.-
• EC 3.5.4.37
• G1P1
• G1P1double-stranded RNA-specific adenosine deaminase
• IFI4
• IFI-4
• IFI4dsRNA adenosine deaminase
• interferon-induced protein 4
• Interferon-inducible protein 4
• K88DSRBP
• p136
• p136,136 kDa double-stranded RNA-binding protein

Background

ADAR encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ADAR Antibody (NBP1-90242)(3)

Publications using NBP1-90242

• Nachmani D, Zimmermann A, Oiknine Djian E et al. MicroRNA Editing Facilitates Immune Elimination of HCMV Infected Cells. PLoS Pathog 2014-02-01 [PMID: 24586166]
• Witman NM, Behm M, Ohman M, Morrison JI. ADAR-related activation of adenosine-to-inosine RNA editing during regeneration. Stem Cells Dev 2013-08-15 [PMID: 23534823]
• Rice GI, Kasher PR, Forte GM et al. Mutations in ADAR1 cause Aicardi-Goutieres syndrome associated with a type I interferon signature. Nat Genet 2012-11-01 [PMID: 23001123]

Bioinformatics

• Gene Symbol: ADAR
• Entrez:
  • 103(Human)
  • 56417(Mouse)
  • 81635(Rat)
• Uniprot:
  • P55265(Human)