Glucuronic Acid Epimerase Antibody - Azide and BSA Free

Western Blot: Glucuronic Acid Epimerase Antibody [H00026035-B01P] - Analysis of GLCE expression in transfected 293T cell line by GLCE polyclonal antibody. Lane 1: GLCE transfected lysate(67.87 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Glucuronic Acid Epimerase Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Glucuronic Acid Epimerase Antibody - Azide and BSA Free (H00026035-B01P) is a polyclonal antibody validated for use in WB. Anti-Glucuronic Acid Epimerase Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: GLCE (NP_056369.1, 1 a.a. - 617 a.a.) full-length human protein. MRCLAARVNYKTLIIICALFTLVTVLLWNKCSSDKAIQFPRRSSSGFRVDGFEKRAAASESNNYMNHVAKQQSEEAFPQEQQKAPPVVGGFNSNVGSKVLGLKYEEIDCLINDEHTIKGRREGNEVFLPFTWVEKYFDVYGKVVQYDGYDRFEFSHSYSKVYAQRAPYHPDGVFMSFEGYNVEVRDRVKCISGVEGVPLSTQWGPQGYFYPIQIAQYGLSHYSKNLTEKPPHIEVYETAEDRDKNKPNDWTVPKGCFMANVADKSRFTNVKQFIAPETSEGVSLQLGNTKDFIISFDLKFLTNGSVSVVLETTEKNQLFTIHYVSNAQLIAFKERDIYYGIGPRTSWSTVTRDLVTDLRKGVGLSNTKAVKPTKIMPKKVVRLIAKGKGFLDNITISTTAHMAAFFAASDWLVRNQDEKGGWPIMVTRKLGEGFKSLEPGWYSAMAQGQAISTLVRAYLLTKDHIFLNSALRATAPYKFLSEQHGVKAVFMNKHDWYEEYPTTPSSFVLNGFMYSLIGLYDLKETAGEKLGKEARSLYERGMESLKAMLPLYDTGSGTIYDLRHFMLGIAPNLARWDYHTTHINQLQLLSTIDESPVFKEFVKRWKSYLKGSRAKHN
• Specificity: Reacts with glucuronic acid epimerase.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: GLCE
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This antibody is reactive against transfected lysate in western blot, and as a detection antibody in ELISA.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24326668)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Glucuronic Acid Epimerase Antibody - Azide and BSA Free

• D-glucuronyl C5-epimerase
• EC 5.1.3
• EC 5.1.3.-
• glucuronic acid epimerase
• glucuronyl C5-epimerase
• Heparin/heparan sulfate:glucuronic acid C5-epimerase
• HSEPIheparin/heparan sulfate-glucuronic acid C5-epimerase
• KIAA0836heparan sulfate epimerase
• UDP-glucuronic acid epimerase

Background

Heparan sulfate (HS) is a negatively charged cell surface polysaccharide required for the biologic activities of circulating extracellular ligands. GLCE is responsible for epimerization of D-glucuronic acid (GlcA) to L-iduronic acid (IdoA) of HS, which endows the nascent polysaccharide chain with the ability to bind growth factors and cytokines (Ghiselli and Agrawal, 2005 [PubMed 15853773]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glucuronic Acid Epimerase Antibody (H00026035-B01P)(2)

Publications using H00026035-B01P

• Gasimli L, Glass CA, Datta P et al. Bioengineering murine mastocytoma cells to produce anticoagulant heparin. Glycobiology. 2014-03-01 [PMID: 24326668] (Mouse)
• Baik JY, Gasimli L, Yang B et al. Metabolic engineering of Chinese hamster ovary cells: towards a bioengineered heparin. Metab Eng. 2012-03-01 [PMID: 22326251] (WB)

Bioinformatics

• Gene Symbol: GLCE
• Entrez:
  • 26035(Human)
• Uniprot:
  • NP_056369.1(Human)