Exostosin-like 2/EXTL2 Antibody

Western Blot: Exostosin-like 2/EXTL2 Antibody [NBP2-16394] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: Exostosin-like 2/EXTL2 Antibody [NBP2-16394] - Paraffin-embedded SC-M1 xenograft. Exostosin-like 2 antibody [N2C3] dilution: 1:500.

• Reactivity: Hu, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Exostosin-like 2/EXTL2 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human EXTL2. The exact sequence is proprietary.
• Localization: Endoplasmic reticulum membrane; Single-pass type II membrane protein; Processed exostosin-like 2: Secreted
• Predicted Species: Rhesus Macaque (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EXTL2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Application Notes: Use in ICC/IF reported in scientific literature (PMID: 30553836).
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (86%), Rat (85%), Pig (85%), Bovine (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Exostosin-like 2/EXTL2 Antibody

• Alpha-1,4-N-acetylhexosaminyltransferase EXTL2
• alpha-1,4-N-acteylhexosaminyltransferase
• Alpha-GalNAcT EXTL2
• EC 2.4.1.223
• exostoses (multiple)-like 2
• Exostosin like 2
• Exostosin-like 2
• EXTL2
• EXTR2
• EXT-related protein 2
• Glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase
• processed exostosin-like 2

Background

Glycosyltransferase required for the biosynthesis of heparan-sulfate and responsible for the alternating addition of beta-1-4-linked glucuronic acid (GlcA) and alpha-1-4-linked N-acetylglucosamine (GlcNAc) units to nascent heparan sulfate chains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Exostosin-like 2/EXTL2 Antibody (NBP2-16394)(1)

Publication using NBP2-16394

• Chanalaris A, Clarke H, Guimond SE et al. Heparan Sulfate Proteoglycan Synthesis is Dysregulated in Human Osteoarthritic Cartilage Am. J. Pathol. 2018-12-13 [PMID: 30553836] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: EXTL2
• Uniprot:
  • Q9UBQ6(Human)