Glucosylceramidase/GBA Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Glucosylceramidase/GBA Antibody [NBP1-32271] - Analysis of methanol-fixed HeLa, using GBA antibody at 1:100 dilution.

Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody [NBP1-32271] - Lung cancer, using GBA antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271] - Sample(30 ug of whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:500

Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271] - MCF-7 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glucosylceramidase/GBA antibody [C1C3] (NBP1-32271) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271] - Wild-type (WT) and GBA knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GBA antibody [C1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Ze, Rt, Po, Bv, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Glucosylceramidase/GBA Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Glucosylceramidase/GBA. The exact sequence is proprietary.
• Localization: Lysosome membrane, Peripheral membrane protein, Lumenal side
• Predicted Species: Rat (91%), Porcine (92%), Chimpanzee (100%), Bovine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GBA1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 25326392). Mouse reactivity reported in scientific literature (PMID: 30927072).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, (20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Glucosylceramidase/GBA Antibody - BSA Free

• Acid beta-glucosidase
• Alglucerase
• beta-glucocerebrosidase
• D-glucosyl-N-acylsphingosine glucohydrolase
• EC 3.2.1.45
• GBA
• GBA1
• GC
• GCB
• GLUC
• glucosidase, beta, acid
• glucosidase, beta; acid (includes glucosylceramidase)
• Glucosylceramidase
• Imiglucerase
• lysosomal glucocerebrosidase

Background

This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants encoding the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Glucosylceramidase/GBA Antibody (NBP1-32271)(2)

Publications using NBP1-32271

• Bieri G, Brahic M, Bousset L et al. LRRK2 modifies alpha-syn pathology and spread in mouse models and human neurons bioRxiv 2019-03-29 [PMID: 30927072] (WB, Mouse)
• Zancan I, Bellesso S, Costa R et al. Glucocerebrosidase deficiency in zebrafish affects primary bone ossification through increased oxidative stress and reduced Wnt/B-catenin signaling. Hum. Mol. Genet. 2014-10-17 [PMID: 25326392] (ICC/IF, WB, Zebrafish, Human)

Bioinformatics

• Gene Symbol: GBA1
• Uniprot:
  • P04062()