Glucagon R/GCGR Antibody - BSA Free

Immunohistochemistry-Paraffin: Glucagon R/GCGR Antibody [NLS4256] - Analysis of anti-Glucagon Receptor antibody with human liver.

• Reactivity: Hu, Rt, Pm, Pm, Eq
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Glucagon R/GCGR Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 18 amino acid peptide from N-terminal extracellular domain of human Glucagon R/GCGR.
• Epitope: N-Terminus extracellular domain
• Localization: Plasma membrane
• Specificity: Human Glucagon R/GCGR. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Equine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GCGR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 13 ug/ml
  • Western Blot
• Application Notes: Use in WB reported in scientific literature (PMID: 23685839)..
• Control:

  Glucagon R/GCGR Overexpression Lysate

Reactivity Notes

Human, Rat reactivity reported in (PMID:23685839). Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Mouse (89%), Hamster (89%), Bovine (89%), Canine (89%).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Glucagon R/GCGR Antibody - BSA Free

• FLJ97182
• GCGR
• GGR
• GLR
• GL-R
• Glucagon R
• glucagon receptor
• GlucagonR
• MGC138246

Background

The Glucagon Receptor mediates the effects of glucagon in controlling glucose metabolism by initiating a cascade of events that regulate the amount of glucose released from the liver into the bloodstream. Glucagon generally functions as a counterregulatory hormone, opposing the actions of insulin, in maintaining the levels of blood glucose, particularly in patients with hypoglycemia. In patients with diabetes, excess glucagon secretion plays a primary role in the metabolic perturbations associated with diabetes, such as hyperglycemia. Glucagon knockout mice exhibit reduced glood glucose levels, improved glucose tolerance, elevated blood glucagon levels, and normal insulin levels. Despite a total absence of glucagon receptors, these animals maintained near-normal glycemia and normal lipidemia, in the presence of circulating glucagon concentrations that were elevated by two orders of magnitude. A missense mutation (Gly40Ser) in the glucagon receptor gene is associated with non-insulin-dependent diabetes mellitus. The glucagon receptor has been reported to be expressed in liver and adipose, but not in adrenal medulla. ESTs have been isolated from liver/spleen and kidney libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glucagon R/GCGR Antibody (NLS4256)(3)

Publications using NLS4256

• Mieczkowska A, Bouvard B, Legrand E, Mabilleau G [Gly2]-GLP-2, But Not Glucagon or [D-Ala2]-GLP-1, Controls Collagen Crosslinking in Murine Osteoblast Cultures Frontiers in endocrinology 2021-08-04 [PMID: 34421828] (WB)
• LaPierre MP, Abraham MA, Yue JT et al. Glucagon signalling in the dorsal vagal complex is sufficient and necessary for high-protein feeding to regulate glucose homeostasis in vivo. EMBO Rep. 2015-08-19 [PMID: 26290496]
• Mighiu PI, Yue JT, Filippi BM et al. Hypothalamic glucagon signaling inhibits hepatic glucose production. Nat Med 2013-06-01 [PMID: 23685839] (WB, Rat, Human)

Bioinformatics

• Gene Symbol: GCGR
• Entrez:
  • 2642(Human)
• Uniprot:
  • P47871()