GLP-1R Antibody

Images

 
Western Blot: GLP-1R Antibody [NBP1-97308] - Analysis of GLP1R in human pancreas cell lysate.
Western Blot: GLP-1R Antibody [NBP1-97308] - Total protein from Human and Mouse brain and SHSY-5Y cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more
Simple Western: GLP-1R Antibody [NBP1-97308] - Simple Western lane view shows a specific band for GLP-1R in 0.5 mg/ml of Human Pancreas (left) and Mouse Pancreas (right) lysate. This experiment was performed under ...read more
Immunohistochemistry: GLP-1R Antibody [NBP1-97308] - Analysis of GLP1R in mouse pancreas using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: GLP-1R Antibody [NBP1-97308] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were ...read more
Immunohistochemistry-Paraffin: GLP-1R Antibody [NBP1-97308] - Tissue section of mouse pancreas using 1:200 dilution of rabbit anti-GLP1R antibody. The staining was developed with HRP labeled anti-rabbit IgG secondary ...read more
Immunocytochemistry/ Immunofluorescence: GLP1R Antibody [NBP1-97308] - GLP1R-1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry-Paraffin: GLP-1R Antibody [NBP1-97308] - Tissue section of mouse pancreas using 1:200 dilution of rabbit anti-GLP1R antibody. The staining was developed with HRP labeled anti-rabbit IgG secondary ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Simple Western, DB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.06 mg/ml

GLP-1R Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human GLP1R protein (between residues 250-350) [UniProt P43220]
Localization
Cell membrane; Multi-pass membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GLP1R
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:100
  • Dot Blot
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200 - 1:400
  • Immunohistochemistry-Paraffin 1:200 - 1:400
Application Notes
This GLP1R antibody is useful for WB, ICC/IF, and IHC-P. In WB, a band is seen ~53kDa representing GLP1R. In ICC/IF, membrane staining was observed in HeLa cells. In IHC-P, strong membrane staining was observed in mouse pancreas tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Dot blot reported in scientific literature (PMID 27435156).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control Peptide
Publications
Read Publications using
NBP1-97308 in the following applications:

  • DB
    1 publication
  • IHC
    6 publications
  • 2 publications
  • WB
    3 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27435156). Canine reactivity reported in scientific literature (PMID: 25747753).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.06 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GLP-1R Antibody

  • GLP-1 receptor
  • GLP1R
  • GLP-1R
  • GLP-1-R
  • glucagon-like peptide 1 receptor
  • MGC138331

Background

GLP1R (Glucagon-like peptide 1 receptor) is an incretin hormone produced in the intestine that is released in response to food intake. GLP1R plays an important role in maintaining blood glucose homeostasis and has been suggested to affect the feelings of satiety or hunger, sensation of glucose levels and control of glucagon sensitivity of islets. In addition, desensitization of GLP1R on pancreatic beta-cells is one of the causes of non insulin-dependent diabetes mellitus (NIDDM), and GLP1R is starting to play an increasingly important role in endocrine gastrointestinal tumor management.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GLP-1R Antibody (NBP1-97308)(12)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: DB, IHC, IHC-P, WB.


Filter By Application
DB
(1)
IHC
(6)
IHC-P
(2)
WB
(3)
All Applications
Filter By Species
Human
(5)
Mouse
(3)
Rat
(3)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NBP1-97308 Applications Species
Iwaya C, Nomiyama T, Komatsu S et al. Exendin-4, a glucagon-like peptide-1 receptor agonist, attenuates breast cancer growth by inhibiting NF-kB activation. Endocrinology. 2017 Oct 13 [PMID: 29045658] (IHC-P, Human) IHC-P Human
Wenjing H, Shuang Y, Weisong L, Haipeng X. Exendin-4 does not modify growth or apoptosis of human colon cancer cells. Endocr. Res. Mar 20 2017 [PMID: 28318339] (IHC, Human) IHC Human
Steven S, Jurk K, Kopp M et al. Glucagon-like peptide-1 receptor signalling reduces microvascular thrombosis, nitrosative and oxidative stress, systemic inflammation and platelet activation in endotoxaemic mice Br. J. Pharmacol. 2016 Jul 19 [PMID: 27435156] (WB, DB, Human, Mouse, Rat) WB, DB Human, Mouse, Rat
Tsutsumi Y, Nomiyama T, Kawanami T et al. Combined Treatment with Exendin-4 and Metformin Attenuates Prostate Cancer Growth. PLoS One 2015 [PMID: 26439622] (IHC) IHC
Ihara M, Asanuma H, Yamazaki S et al. An interaction between glucagon-like peptide-1 and adenosine contributes to cardioprotection of a dipeptidyl peptidase 4 inhibitor from myocardial ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol 2015 May 15 [PMID: 25747753]
Nomiyama T, Yanase, T. GLP-1 receptor agonist as treatment for cancer as well as diabetes: beyond blood glucose control. Expert Review of Endocrinology & Metabolism. May 23 2016
Cappetta D, Ciuffreda L, Cozzolino A et al. Dipeptidyl Peptidase 4 Inhibition Ameliorates Chronic Kidney Disease in a Model of Salt-Dependent Hypertension Oxidative Medicine and Cellular Longevity Jan 10 2019 [PMID: 30774748] (IHC, Rat) IHC Rat
Zhou D, Chen YW, Zhao ZH et al. Sodium butyrate reduces high-fat diet-induced non-alcoholic steatohepatitis through upregulation of hepatic GLP-1R expression Exp. Mol. Med. Dec 3 2018 [PMID: 30510243] (IHC-P, WB, Human) IHC-P, WB Human
Zhou Y, Li Z, Cao X et al. Exendin-4 Improves Behaviorial Deficits via GLP-1/GLP-1R Signaling following Partial Hepatectomy. Oncogenesis. Nov 12 2018 [PMID: 30408479] (IHC, Rat) IHC Rat
Rocha-Ferreira E, Poupon L, Zelco A et al. Neuroprotective exendin-4 enhances hypothermia therapy in a model of hypoxic-ischaemic encephalopathy Brain Aug 24 2018 [PMID: 30165597] (IHC, Mouse) IHC Mouse
Show All 12 Publications.

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Product General Protocols

View specific protocols for GLP-1R Antibody (NBP1-97308): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GLP-1R Antibody (NBP1-97308). (Showing 1 - 2 of 2 FAQs).

  1. If possible I would like to get more information on the immunogen used to rase NBP1-97308.
    • The immunogen for NBP1-97308 is considered to be proprietary; however the range of amino acids where the immunogen is located is between residues 250-350. [UniProt P43220].
  2. I am wondering if I can use it for in situ PLA to test interaction with a GPCR scaffolding protein, i.e. the secondary antibody should be exposed to the cytosol. Is this antibody suitable?
    • NBP1-97308 recognizes an extracellular portion of GLP1R so it does not seem that it is suitable for your studies, as described.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-97308AF350
Alexa Fluor 405 NBP1-97308AF405
Alexa Fluor 488 NBP1-97308AF488
Alexa Fluor 532 NBP1-97308AF532
Alexa Fluor 594 NBP1-97308AF594
Alexa Fluor 647 NBP1-97308AF647
Alexa Fluor 700 NBP1-97308AF700
Alexa Fluor 750 NBP1-97308AF750
Biotin NBP1-97308B
DyLight 350 NBP1-97308UV
DyLight 405 NBP1-97308V
DyLight 488 NBP1-97308G
DyLight 550 NBP1-97308R
DyLight 594 NBP1-97308DL594
DyLight 650 NBP1-97308C
DyLight 680 NBP1-97308FR
DyLight 755 NBP1-97308IR
FITC NBP1-97308F
HRP NBP1-97308H
Janelia Fluor 549 NBP1-97308JF549
Janelia Fluor 646 NBP1-97308JF646

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Bioinformatics

Gene Symbol GLP1R
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