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GLP-1R Antibody - BSA Free

Images

 
Immunohistochemistry: GLP-1R Antibody [NBP1-97308] - Analysis of GLP1R in mouse pancreas using DAB with hematoxylin counterstain.
Biological Strategies: Knockdown Validated: GLP-1R Antibody [NBP1-97308] - Liraglutide reduces apoptosis of hCMSCs via PKA/i2-catenin pathway. The expression of GLP-1R and apoptotic proteins Bax, Bcl-2, cleaved ...read more
Simple Western: GLP-1R Antibody [NBP1-97308] - Simple Western lane view shows a specific band for GLP-1R in 0.5 mg/ml of Human Pancreas (left) and Mouse Pancreas (right) lysate. This experiment was performed under ...read more
Western Blot: GLP-1R Antibody [NBP1-97308] - Total protein from Human and Mouse brain and SHSY-5Y cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more
Biological Strategies: Western Blot: GLP-1R Antibody [NBP1-97308] - Expression of GLP-1R in three MSCs at different time points under the stimulation of LPS. The expression of GLP-1R in hCMSCs, hBMSCs, and hAMSCs ...read more
Immunocytochemistry/ Immunofluorescence: GLP-1R Antibody [NBP1-97308] - GLP1R-1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry-Paraffin: GLP-1R Antibody [NBP1-97308] - Tissue section of mouse pancreas using 1:200 dilution of rabbit anti-GLP1R antibody. The staining was developed with HRP labeled anti-rabbit IgG secondary ...read more
Western Blot: GLP-1R Antibody [NBP1-97308] - Analysis of GLP1R in human pancreas cell lysate.
Immunocytochemistry/ Immunofluorescence: GLP-1R Antibody [NBP1-97308] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were ...read more
Immunohistochemistry-Paraffin: GLP-1R Antibody [NBP1-97308] - Tissue section of mouse pancreas using 1:200 dilution of rabbit anti-GLP1R antibody. The staining was developed with HRP labeled anti-rabbit IgG secondary ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Simple Western, DB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

GLP-1R Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal portion of the human GLP1R protein (between residues 250-350) [UniProt P43220]
Localization
Cell membrane; Multi-pass membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GLP1R
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dot Blot reported in scientific literature (PMID 27435156)
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200 - 1:400
  • Knockdown Validated reported in scientific literature (PMID 31900217)
  • Simple Western 1:100
  • Western Blot 1:1000
Application Notes
In WB, a band is seen ~53kDa representing GLP1R. In ICC/IF, membrane staining was observed in HeLa cells. In IHC-P, strong membrane staining was observed in mouse pancreas tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control Peptide
GLP-1R Peptide (NBP1-97308PEP)
Publications
Read Publications using
NBP1-97308 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27435156). Canine reactivity reported in scientific literature (PMID: 25747753).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GLP-1R Antibody - BSA Free

  • GLP-1 receptor
  • GLP1R
  • GLP-1R
  • GLP-1-R
  • glucagon-like peptide 1 receptor
  • MGC138331

Background

GLP1R (Glucagon-like peptide 1 receptor) is an incretin hormone produced in the intestine that is released in response to food intake. GLP1R plays an important role in maintaining blood glucose homeostasis and has been suggested to affect the feelings of satiety or hunger, sensation of glucose levels and control of glucagon sensitivity of islets. In addition, desensitization of GLP1R on pancreatic beta-cells is one of the causes of non insulin-dependent diabetes mellitus (NIDDM), and GLP1R is starting to play an increasingly important role in endocrine gastrointestinal tumor management.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GLP-1R Antibody (NBP1-97308)(27)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: Cytometric Bead Assay Standard, ICC/IF, IF/IHC, IHC-P, KD, WB.


Filter By Application
Cytometric Bead Assay Standard
(1)
ICC/IF
(2)
IF/IHC
(9)
IHC-P
(4)
KD
(1)
WB
(12)
All Applications
Filter By Species
Human
(10)
Mouse
(10)
Rat
(3)
All Species
Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using NBP1-97308 Applications Species
Cantacorps L, Coull BM, Falck J et al. Gut-derived peptide hormone receptor expression in the developing mouse hypothalamus PLOS ONE 2023-08-17 [PMID: 37590249] (WB) WB
You F, Li C, Zhang S et al. Sitagliptin inhibits the survival, stemness and autophagy of glioma cells, and enhances temozolomide cytotoxicity Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023-03-24 [PMID: 36966667] (WB, Human)

Details:
Dilution used in WB 1:1000
WB Human
Wang K, Cui X, Li F et al. Glucagon receptor blockage inhibits beta -cell dedifferentiation through FoxO1 American journal of physiology. Endocrinology and metabolism 2022-11-16 [PMID: 36383639] (IHC-P, WB, Mouse) IHC-P, WB Mouse
Li R, Ye Z, She D et al. Semaglutide May Alleviate Hepatic Steatosis in T2DM Combined with NFALD Mice via miR-5120/ABHD6 Drug design, development and therapy 2022-10-12 [PMID: 36238196] (IHC-P, Mouse) IHC-P Mouse
Li R, She D, Ye Z et al. Glucagon-Like Peptide 1 Receptor Agonist Improves Renal Tubular Damage in Mice with Diabetic Kidney Disease Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy 2022-04-29 [PMID: 35519661] (ICC/IF) ICC/IF
Meurot C, Martin C, Sudre L et al. Liraglutide, a glucagon-like peptide 1 receptor agonist, exerts analgesic, anti-inflammatory and anti-degradative actions in osteoarthritis Scientific reports 2022-01-28 [PMID: 35091584] (IF/IHC, Human) IF/IHC Human
Li Y, Xu B, Yang J Et al. Liraglutide protects against lethal renal ischemia-reperfusion injury by inhibiting high-mobility group box 1 nuclear-cytoplasmic translocation and release Pharmacological Research 2021-09-01 [PMID: 34481074] (IF/IHC, WB, Mouse) IF/IHC, WB Mouse
Mieczkowska A, Bouvard B, Legrand E, Mabilleau G [Gly2]-GLP-2, But Not Glucagon or [D-Ala2]-GLP-1, Controls Collagen Crosslinking in Murine Osteoblast Cultures Frontiers in endocrinology 2021-08-04 [PMID: 34421828] (WB) WB
Sho H, Fukui K, Yoneda S et al. Insulinoma induces a hyperinsulinemia-mediated decrease of GLUT2 and GLP1 receptor in normal pancreatic beta-cells Biochem Biophys Res Commun 2020-11-13 [PMID: 33199025] (WB, Mouse)

Details:
Western blot analysis performed on Min6 cells cultured in insulin
WB Mouse
Deng H, Yang F, Ma X et al. Long-Term Liraglutide Administration Induces Pancreas Neogenesis in Adult T2DM Mice Cell Transplant 2020-06-26 [PMID: 32584149] (ICC/IF, Mouse) ICC/IF Mouse
Show All 27 Publications.

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Product General Protocols

View specific protocols for GLP-1R Antibody (NBP1-97308): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GLP-1R Antibody (NBP1-97308). (Showing 1 - 2 of 2 FAQs).

  1. If possible I would like to get more information on the immunogen used to rase NBP1-97308.
    • The immunogen for NBP1-97308 is considered to be proprietary; however the range of amino acids where the immunogen is located is between residues 250-350. [UniProt P43220].
  2. I am wondering if I can use it for in situ PLA to test interaction with a GPCR scaffolding protein, i.e. the secondary antibody should be exposed to the cytosol. Is this antibody suitable?
    • NBP1-97308 recognizes an extracellular portion of GLP1R so it does not seem that it is suitable for your studies, as described.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GLP1R
Entrez
Uniprot