Western Blot: GLP1R Antibody [NBP1-97308] - WB analysis of GLP1R in human pancreas cell lysate.
Immunocytochemistry/ Immunofluorescence: GLP1R Antibody [NBP1-97308] - GLP1R-1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: GLP1R Antibody [NBP1-97308] - IHC analysis of GLP1R in mouse pancreas using DAB with hematoxylin counterstain.
Simple Western: GLP-1R Antibody [NBP1-97308] - Simple Western lane view shows a specific band for GLP-1R in 0.5 mg/ml of Human Pancreas (left) and Mouse Pancreas (right) lysate. This experiment was performed under ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 30% glycerol
0.05% Sodium Azide
Immunogen affinity purified
Western Blot 1:1000
Simple Western 1:100
This GLP1R antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and IHC-paraffin embedded sections. In Western Blot, a band is seen ~53kDa representing GLP1R. In ICC/IF, membrane staining was observed in HeLa cells. In IHC-P, strong membrane staining was observed in mouse pancreas tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10-15 uL of the recommended dilution is used per data point. Use in Dot blot reported in scientific literature (PMID 27435156).
Read Publications using NBP1-97308 in the following applications:
Human and mouse. Rat reactivity reported in scientific literature (PMID: 27435156)
Alternate Names for GLP-1R Antibody
glucagon-like peptide 1 receptor
GLP1R (Glucagon-like peptide 1 receptor) is an incretin hormone produced in the intestine that is released in response to food intake. GLP1R plays an important role in maintaining blood glucose homeostasis and has been suggested to affect the feelings of satiety or hunger, sensation of glucose levels and control of glucagon sensitivity of islets. In addition, desensitization of GLP1R on pancreatic beta-cells is one of the causes of non insulin-dependent diabetes mellitus (NIDDM), and GLP1R is starting to play an increasingly important role in endocrine gastrointestinal tumor management.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.