GLP-1R Antibody

Images

 
Immunohistochemistry: GLP-1R Antibody [NBP1-97308] - Analysis of GLP1R in mouse pancreas using DAB with hematoxylin counterstain.
Biological Strategies: Knockdown Validated: GLP-1R Antibody [NBP1-97308] - Liraglutide reduces apoptosis of hCMSCs via PKA/i2-catenin pathway. The expression of GLP-1R and apoptotic proteins Bax, Bcl-2, cleaved ...read more
Simple Western: GLP-1R Antibody [NBP1-97308] - Simple Western lane view shows a specific band for GLP-1R in 0.5 mg/ml of Human Pancreas (left) and Mouse Pancreas (right) lysate. This experiment was performed under ...read more
Western Blot: GLP-1R Antibody [NBP1-97308] - Total protein from Human and Mouse brain and SHSY-5Y cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more
Biological Strategies: Western Blot: GLP-1R Antibody [NBP1-97308] - Expression of GLP-1R in three MSCs at different time points under the stimulation of LPS. The expression of GLP-1R in hCMSCs, hBMSCs, and hAMSCs ...read more
Immunocytochemistry/ Immunofluorescence: GLP-1R Antibody [NBP1-97308] - GLP1R-1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry-Paraffin: GLP-1R Antibody [NBP1-97308] - Tissue section of mouse pancreas using 1:200 dilution of rabbit anti-GLP1R antibody. The staining was developed with HRP labeled anti-rabbit IgG secondary ...read more
Western Blot: GLP-1R Antibody [NBP1-97308] - Analysis of GLP1R in human pancreas cell lysate.
Immunocytochemistry/ Immunofluorescence: GLP-1R Antibody [NBP1-97308] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were ...read more
Immunohistochemistry-Paraffin: GLP-1R Antibody [NBP1-97308] - Tissue section of mouse pancreas using 1:200 dilution of rabbit anti-GLP1R antibody. The staining was developed with HRP labeled anti-rabbit IgG secondary ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Simple Western, DB, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

GLP-1R Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human GLP1R protein (between residues 250-350) [UniProt P43220]
Localization
Cell membrane; Multi-pass membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GLP1R
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:100
  • Dot Blot
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200 - 1:400
  • Knockdown Validated
Application Notes
This GLP1R antibody is useful for WB, ICC/IF, and IHC-P. In WB, a band is seen ~53kDa representing GLP1R. In ICC/IF, membrane staining was observed in HeLa cells. In IHC-P, strong membrane staining was observed in mouse pancreas tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Dot blot reported in scientific literature (PMID 27435156). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Knockdown validation (PMID: 31900217).
Control Peptide
GLP-1R Peptide (NBP1-97308PEP)
Publications
Read Publications using
NBP1-97308 in the following applications:

  • DB
    1 publication
  • 1 publication
  • IHC
    7 publications
  • 2 publications
  • KD
    1 publication
  • WB
    7 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27435156). Canine reactivity reported in scientific literature (PMID: 25747753).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GLP-1R Antibody

  • GLP-1 receptor
  • GLP1R
  • GLP-1R
  • GLP-1-R
  • glucagon-like peptide 1 receptor
  • MGC138331

Background

GLP1R (Glucagon-like peptide 1 receptor) is an incretin hormone produced in the intestine that is released in response to food intake. GLP1R plays an important role in maintaining blood glucose homeostasis and has been suggested to affect the feelings of satiety or hunger, sensation of glucose levels and control of glucagon sensitivity of islets. In addition, desensitization of GLP1R on pancreatic beta-cells is one of the causes of non insulin-dependent diabetes mellitus (NIDDM), and GLP1R is starting to play an increasingly important role in endocrine gastrointestinal tumor management.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-97308
Species: Hu, Mu, Rt, Ca
Applications: WB, Simple Western, DB, ICC/IF, IHC, IHC-P, KD

Publications for GLP-1R Antibody (NBP1-97308)(19)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: DB, ICC/IF, IHC, IHC-P, KD, WB.


Filter By Application
DB
(1)
ICC/IF
(1)
IHC
(7)
IHC-P
(2)
KD
(1)
WB
(7)
All Applications
Filter By Species
Human
(8)
Mouse
(7)
Rat
(3)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NBP1-97308 Applications Species
Sho H, Fukui K, Yoneda S et al. Insulinoma induces a hyperinsulinemia-mediated decrease of GLUT2 and GLP1 receptor in normal pancreatic beta-cells Biochem Biophys Res Commun Nov 13 2020 [PMID: 33199025] (WB, Mouse)

Details:
Western blot analysis performed on Min6 cells cultured in insulin
WB Mouse
Deng H, Yang F, Ma X et al. Long-Term Liraglutide Administration Induces Pancreas Neogenesis in Adult T2DM Mice Cell Transplant Jun 26 2020 [PMID: 32584149] (ICC/IF, Mouse) ICC/IF Mouse
Timper K, Del Rio-Martin A, Cremer AL et al. GLP-1 Receptor Signaling in Astrocytes Regulates Fatty Acid Oxidation, Mitochondrial Integrity, and Function Cell Metab. Jun 2 2020 [PMID: 32433922] (WB, Mouse) WB Mouse
Yang X, Ma X, Don O et al. Mesenchymal stem cells combined with liraglutide relieve acute lung injury through apoptotic signaling restrained by PKA/beta-catenin Stem Cell Res Ther May 19 2020 [PMID: 32429994] (WB, Human) WB Human
Chaudhari S, Yazdizadeh Shotorbani P, Tao Y et al. Inhibition of interleukin-6 on matrix protein production by glomerular mesangial cells and its pathway involved Am. J. Physiol. Renal Physiol. May 11 2020 [PMID: 32390515]
Feng Y, Wang L, Ma X et al. Effect of hCMSCs and liraglutide combination in ALI through cAMP/PKAc/beta-catenin signaling pathway Stem Cell Res Ther Jan 3 2020 [PMID: 31900217] (WB, KD, Human, Mouse) WB, KD Human, Mouse
Cappetta D, Ciuffreda L, Cozzolino A et al. Dipeptidyl Peptidase 4 Inhibition Ameliorates Chronic Kidney Disease in a Model of Salt-Dependent Hypertension Oxidative Medicine and Cellular Longevity Jan 10 2019 [PMID: 30774748] (IHC, Rat) IHC Rat
Zhou D, Chen YW, Zhao ZH et al. Sodium butyrate reduces high-fat diet-induced non-alcoholic steatohepatitis through upregulation of hepatic GLP-1R expression Exp. Mol. Med. Dec 3 2018 [PMID: 30510243] (IHC-P, WB, Human) IHC-P, WB Human
Zhou Y, Li Z, Cao X et al. Exendin-4 Improves Behaviorial Deficits via GLP-1/GLP-1R Signaling following Partial Hepatectomy. Oncogenesis. Nov 12 2018 [PMID: 30408479] (IHC, Rat) IHC Rat
Rocha-Ferreira E, Poupon L, Zelco A et al. Neuroprotective exendin-4 enhances hypothermia therapy in a model of hypoxic-ischaemic encephalopathy Brain Aug 24 2018 [PMID: 30165597] (IHC, Mouse) IHC Mouse
Show All 19 Publications.

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Product General Protocols

View specific protocols for GLP-1R Antibody (NBP1-97308): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GLP-1R Antibody (NBP1-97308). (Showing 1 - 2 of 2 FAQs).

  1. If possible I would like to get more information on the immunogen used to rase NBP1-97308.
    • The immunogen for NBP1-97308 is considered to be proprietary; however the range of amino acids where the immunogen is located is between residues 250-350. [UniProt P43220].
  2. I am wondering if I can use it for in situ PLA to test interaction with a GPCR scaffolding protein, i.e. the secondary antibody should be exposed to the cytosol. Is this antibody suitable?
    • NBP1-97308 recognizes an extracellular portion of GLP1R so it does not seem that it is suitable for your studies, as described.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-97308AF350
Alexa Fluor 405 NBP1-97308AF405
Alexa Fluor 488 NBP1-97308AF488
Alexa Fluor 532 NBP1-97308AF532
Alexa Fluor 594 NBP1-97308AF594
Alexa Fluor 647 NBP1-97308AF647
Alexa Fluor 700 NBP1-97308AF700
Alexa Fluor 750 NBP1-97308AF750
Biotin NBP1-97308B
DyLight 350 NBP1-97308UV
DyLight 405 NBP1-97308V
DyLight 488 NBP1-97308G
DyLight 550 NBP1-97308R
DyLight 594 NBP1-97308DL594
DyLight 650 NBP1-97308C
DyLight 680 NBP1-97308FR
DyLight 755 NBP1-97308IR
FITC NBP1-97308F
HRP NBP1-97308H
Janelia Fluor 549 NBP1-97308JF549
Janelia Fluor 646 NBP1-97308JF646

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Bioinformatics

Gene Symbol GLP1R
Entrez
Uniprot