Reactivity | Hu, Mu, Rt, CaSpecies Glossary |
Applications | WB, Simple Western, DB, ICC/IF, IHC, IHC-P, KD |
Clonality | Polyclonal |
Host | Rabbit |
Conjugate | Unconjugated |
Format | BSA Free |
Concentration | 1.0 mg/ml |
Immunogen | A synthetic peptide made to an internal portion of the human GLP1R protein (between residues 250-350) [UniProt P43220] |
Localization | Cell membrane; Multi-pass membrane protein. |
Isotype | IgG |
Clonality | Polyclonal |
Host | Rabbit |
Gene | GLP1R |
Purity | Immunogen affinity purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | This GLP1R antibody is useful for WB, ICC/IF, and IHC-P. In WB, a band is seen ~53kDa representing GLP1R. In ICC/IF, membrane staining was observed in HeLa cells. In IHC-P, strong membrane staining was observed in mouse pancreas tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Dot blot reported in scientific literature (PMID 27435156). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Knockdown validation (PMID: 31900217). |
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Control Peptide |
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Publications |
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Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer | PBS |
Preservative | 0.02% Sodium Azide |
Concentration | 1.0 mg/ml |
Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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