GLP-1R Antibody

Western Blot: GLP-1R Antibody [NBP1-97308] - Analysis of GLP1R in human pancreas cell lysate.

Western Blot: GLP-1R Antibody [NBP1-97308] - Total protein from Human and Mouse brain and SHSY-5Y cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-GLP1R in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Simple Western: GLP-1R Antibody [NBP1-97308] - Simple Western lane view shows a specific band for GLP-1R in 0.5 mg/ml of Human Pancreas (left) and Mouse Pancreas (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody

Immunohistochemistry: GLP-1R Antibody [NBP1-97308] - Analysis of GLP1R in mouse pancreas using DAB with hematoxylin counterstain.

Immunocytochemistry/Immunofluorescence: GLP-1R Antibody [NBP1-97308] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-GLP-1R at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: GLP-1R Antibody [NBP1-97308] - Tissue section of mouse pancreas using 1:200 dilution of rabbit anti-GLP1R antibody. The staining was developed with HRP labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This GLP1R antibody primarily generated a specific membrane cytoplasmic staining of apparently beta cells in the Islets of Langerhans. The cells of lobular/inter-lobular ducts and the acinar cells were largely negative for GLP1R.

Immunocytochemistry/Immunofluorescence: GLP1R Antibody [NBP1-97308] - GLP1R-1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry-Paraffin: GLP-1R Antibody [NBP1-97308] - Tissue section of mouse pancreas using 1:200 dilution of rabbit anti-GLP1R antibody. The staining was developed with HRP labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This GLP1R antibody primarily generated a specific membrane cytoplasmic staining of apparently beta cells in the Islets of Langerhans.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, Simple Western, DB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.06 mg/ml

GLP-1R Antibody Summary

• Immunogen: A synthetic peptide made to an internal portion of the human GLP1R protein (between residues 250-350) [UniProt P43220]
• Localization: Cell membrane; Multi-pass membrane protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GLP1R
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • Simple Western 1:100
  • Dot Blot
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200 - 1:400
  • Immunohistochemistry-Paraffin 1:200 - 1:400
• Application Notes: This GLP1R antibody is useful for WB, ICC/IF, and IHC-P. In WB, a band is seen ~53kDa representing GLP1R. In ICC/IF, membrane staining was observed in HeLa cells. In IHC-P, strong membrane staining was observed in mouse pancreas tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Dot blot reported in scientific literature (PMID 27435156).
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27435156). Canine reactivity reported in scientific literature (PMID: 25747753).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.06 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GLP-1R Antibody

• GLP-1 receptor
• GLP1R
• GLP-1R
• GLP-1-R
• glucagon-like peptide 1 receptor
• MGC138331

Background

GLP1R (Glucagon-like peptide 1 receptor) is an incretin hormone produced in the intestine that is released in response to food intake. GLP1R plays an important role in maintaining blood glucose homeostasis and has been suggested to affect the feelings of satiety or hunger, sensation of glucose levels and control of glucagon sensitivity of islets. In addition, desensitization of GLP1R on pancreatic beta-cells is one of the causes of non insulin-dependent diabetes mellitus (NIDDM), and GLP1R is starting to play an increasingly important role in endocrine gastrointestinal tumor management.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GLP-1R Antibody (NBP1-97308)(12)

Publications using NBP1-97308

• Iwaya C, Nomiyama T, Komatsu S et al. Exendin-4, a glucagon-like peptide-1 receptor agonist, attenuates breast cancer growth by inhibiting NF-kB activation. Endocrinology. 2017 Oct 13 [PMID: 29045658] (IHC-P, Human)
• Wenjing H, Shuang Y, Weisong L, Haipeng X. Exendin-4 does not modify growth or apoptosis of human colon cancer cells. Endocr. Res. Mar 20 2017 [PMID: 28318339] (IHC, Human)
• Steven S, Jurk K, Kopp M et al. Glucagon-like peptide-1 receptor signalling reduces microvascular thrombosis, nitrosative and oxidative stress, systemic inflammation and platelet activation in endotoxaemic mice Br. J. Pharmacol. 2016 Jul 19 [PMID: 27435156] (WB, DB, Human, Mouse, Rat)
• Tsutsumi Y, Nomiyama T, Kawanami T et al. Combined Treatment with Exendin-4 and Metformin Attenuates Prostate Cancer Growth. PLoS One 2015 [PMID: 26439622] (IHC)
• Ihara M, Asanuma H, Yamazaki S et al. An interaction between glucagon-like peptide-1 and adenosine contributes to cardioprotection of a dipeptidyl peptidase 4 inhibitor from myocardial ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol 2015 May 15 [PMID: 25747753]
• Nomiyama T, Yanase, T. GLP-1 receptor agonist as treatment for cancer as well as diabetes: beyond blood glucose control. Expert Review of Endocrinology & Metabolism. May 23 2016
• Cappetta D, Ciuffreda L, Cozzolino A et al. Dipeptidyl Peptidase 4 Inhibition Ameliorates Chronic Kidney Disease in a Model of Salt-Dependent Hypertension Oxidative Medicine and Cellular Longevity Jan 10 2019 [PMID: 30774748] (IHC, Rat)
• Zhou D, Chen YW, Zhao ZH et al. Sodium butyrate reduces high-fat diet-induced non-alcoholic steatohepatitis through upregulation of hepatic GLP-1R expression Exp. Mol. Med. Dec 3 2018 [PMID: 30510243] (IHC-P, WB, Human)
• Zhou Y, Li Z, Cao X et al. Exendin-4 Improves Behaviorial Deficits via GLP-1/GLP-1R Signaling following Partial Hepatectomy. Oncogenesis. Nov 12 2018 [PMID: 30408479] (IHC, Rat)
• Rocha-Ferreira E, Poupon L, Zelco A et al. Neuroprotective exendin-4 enhances hypothermia therapy in a model of hypoxic-ischaemic encephalopathy Brain Aug 24 2018 [PMID: 30165597] (IHC, Mouse)
• He W, Yu S, Wang L et al. Exendin-4 inhibits growth and augments apoptosis of ovarian cancer cells. Mol. Cell. Endocrinol. Aug 3 2016 [PMID: 27496641] (IHC, Human)
• Kedees MH, Guz Y, Grigoryan M et al. Functional activity of murine intestinal mucosal cells is regulated by the glucagon-like peptide-1 receptor Peptides. 2013 Aug 6. pii: S0196-9781(13)00262-3. August 6, 2013 [PMID: 23927844] (WB, Mouse)

FAQs for GLP-1R Antibody (NBP1-97308). (Showing 1 - 2 of 2 FAQs).

1. If possible I would like to get more information on the immunogen used to rase NBP1-97308.
   • The immunogen for NBP1-97308 is considered to be proprietary; however the range of amino acids where the immunogen is located is between residues 250-350. [UniProt P43220].
2. I am wondering if I can use it for in situ PLA to test interaction with a GPCR scaffolding protein, i.e. the secondary antibody should be exposed to the cytosol. Is this antibody suitable?
   • NBP1-97308 recognizes an extracellular portion of GLP1R so it does not seem that it is suitable for your studies, as described.

Bioinformatics

• Gene Symbol: GLP1R
• Entrez:
  • 2740(Human)
• Uniprot:
  • P43220()