GLI-2 Antibody [Biotin]

GLI-2 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Sheep Anti-Human GLI-2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3526) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

GLI-2 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human GLI-2
  Ala2-Glu91
  Accession # BAA03568
• Specificity: Detects human GLI-2 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse GLI-2 is observed and 5% cross-reactivity with recombinant human (rh) GLI-1 and rhGLI-3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: GLI2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GLI-2 Antibody [Biotin]

• GLI family zinc finger 2
• GLI2
• GLI-2
• GLI-Kruppel family member GLI2
• glioma-associated oncogene family zinc finger 2
• HPE9tax helper protein 1
• oncogene GLI2
• tax helper protein 2
• Tax helper protein
• tax-responsive element-2 holding protein
• tax-responsive element-25-bp sequence binding protein
• THP
• THP1
• THP2
• zinc finger protein GLI2

Background

GLI-2 is a 166 kDa biopotential transcription regulator of the hedgehog signaling pathway. It contains 5 conserved tandem C2H2 zinc finger domains that are flanked by a repression domain at the N-terminal region and an activation domain in the C-terminal region. At least four isoforms that differ in their N- or C-terminal regions have been described. Transcriptional activity of GLI-2 is regulated by proteolytic processing and degradation. Within the region used as immunogen, human and mouse GLI-2 share 88% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GLI2
• Uniprot:
  • BAA03568()