GLI-1 Antibody

Images

 
Western Blot: GLI-1 Antibody [NBP2-24662] - Analysis of human kidney lysate using 1) pre-immune sera at 1:5000 dilution and 2) GLI-1 antibody at 1 ug/ml. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL ...read more
Immunocytochemistry/ Immunofluorescence: GLI-1 Antibody [NBP2-24662] - staining of GLI-1 at 10 ug/ml. Top panel: C666-1 malignant human nasopharyngel carcinoma cell line. Bottom panel: OKF6 tert-1 human oral epithelial ...read more
Immunohistochemistry-Paraffin: GLI-1 Antibody [NBP2-24662] - Formalin-fixed, paraffin-embedded human brain tissue stained with an isotype control antibody (top) and GLI-1 antibody (bottom) at 5 ug/ml.

Product Details

Summary
Reactivity Hu, EqSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

GLI-1 Antibody Summary

Immunogen
A portion of amino acids 940-990 of human GLI1 was used as the immunogen for this antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GLI1
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-3 ug/ml

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.1 ml PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for GLI-1 Antibody

  • GLI family zinc finger 1
  • GLI1
  • GLI-1
  • glioma-associated oncogene family zinc finger 1
  • glioma-associated oncogene homolog 1 (zinc finger protein)
  • Glioma-associated oncogene
  • GLIzinc finger protein GLI1
  • Oncogene GLI

Background

GLI1 is a protein originally isolated in human glioblastoma. The GLI proteins are the effectors of Sonic Hedgehog (Hh) signaling and have been shown to be involved in cell-fate determination, proliferation and patterning in many cell types and most organs during embryo development. GLI1 was originally isolated from a glioma tumour and has been found to be up regulated in many tumors including muscle, brain and skin tumors such as Basal cell carcinoma (BCC). Shh and the Gli genes are normally expressed in hair follicles, and skin tumours expressing GLI1 may arise from hair follicles. The level of GLI1 expression correlates with the tumor grade in bone and soft tissue sarcomas. GLI1 expression can be cytoplasm and or nucleus. The GLI1 protein is tethered in the cytoplasm by binding to SUFU. Activation and translocation of the GLI1 protein to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is also promoted by interaction with ZIC1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for GLI-1 Antibody (NBP2-24662): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GLI-1 Antibody (NBP2-24662). (Showing 1 - 1 of 1 FAQ).

  1. I would like to know what the stock concentration / volume is for NBP2-24662 lot # 920852.
    • Concentration: 0.5mg/ml

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics

Gene Symbol GLI1