GITR/TNFRSF18 Antibody (110416) [Allophycocyanin]

Human peripheral blood CD4+lymphocytes treated with PHA for 5 days were stained with Mouse Anti-Human GITR/TNFRSF18 APC-conjugated Monoclonal Antibody (Catalog # FAB689A, filled histogram) or isotype control antibody (IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

RPMI8226 (positive cell line)/Jurkat (negative cell line) were stained with Mouse Anti-Human GITR/TNFRSF18 APC‑conjugated Monoclonal Antibody (Catalog # FAB689A, filled histogram) or , open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 110416
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

GITR/TNFRSF18 Antibody (110416) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human GITR/TNFRSF18
  Gln26-Glu161 (Thr43Ala)
  Accession # Q9Y5U5
• Specificity: Detects human GITR in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) 4-1BB, recombinant mouse (rm) 4-1BB, rhCD27, rmCD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rhEDAR, rmEDAR, rhFas, rmFAS, rmGITR, rhHVEM, rhLymphotoxin R beta , rmLymphotoxin R beta , rhNGF R, rhOPG, rmOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rmTNF RI, rhTNF RII, or rmTNF RII.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFRSF18
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  12 months from date of receipt, 2 to 8 degreesC as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GITR/TNFRSF18 Antibody (110416) [Allophycocyanin]

• Activation-inducible TNFR family receptor
• AITR
• AITRTNF receptor superfamily activation-inducible protein
• CD357 antigen
• CD357
• GITR
• GITR-D
• GITRtumor necrosis factor receptor superfamily member 18
• Glucocorticoid-induced TNFR-related protein
• TNFRSF18
• tumor necrosis factor receptor superfamily, member 18

Background

GITR (glucocorticoid-induced tumor necrosis factor receptor, also named AITR, activation-inducible TNF R family member), is a 228 amino acid (aa) type I transmembrane protein belonging to the TNF R family and has been designated TNFRSF18. The GITR cytoplasmic domain has striking homology with the cytoplasmic domain of 4-1BB and CD27. Human GITR shares 55% homology with murine GITR. GITR is expressed at low levels in peripheral blood T cells, bone marrow, thymus, spleen, and lymph nodes. In contrast to mouse GITR, expression of human GITR is not induced by treatment with dexamethasone, but is up-regulated by antigen stimulation or by treatment with anti-CD3 plus anti-CD28, or PMA plus ionomycin. Human GITR ligand was identified from human umbilical vein endothelial cells and is a 177 aa polypeptide belonging to the TNF superfamily (TNFSF18). Ligation of GITR can activate NF-kappa B through TRAF2, and protect T cells from TCR activation-induced cell death. It has been proposed that GITR ligand and GITR may modulate T lymphocyte functions.

1. Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
2. Kwon, B. et al. (1999) J. Biol. Chem. 274:6056.
3. Gurney, A.L. et al. (1999) Current Biology 9:215.
4. Kwon, B. et al. (1999) Current Opinion in Immunology 11:340.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GITR/TNFRSF18 Antibody (FAB689A)(4)

Publications using FAB689A

• Anne-Kristin Heninger, Anke Theil, Carmen Wilhelm, Cathleen Petzold, Nicole Huebel, Karsten Kretschmer et al. IL-7 Abrogates Suppressive Activity of Human CD4+CD25+FOXP3+ Regulatory T Cells and Allows Expansion of Alloreactive and Autoreactive T Cells The Journal of Immunology 2012-12-15 [PMID: 23129754]
• Perez SA, Karamouzis MV, Skarlos DV, Ardavanis A, Sotiriadou NN, Iliopoulou EG, Salagianni ML, Orphanos G, Baxevanis CN, Rigatos G, Papamichail M CD4+CD25+ regulatory T-cell frequency in HER-2/neu (HER)-positive and HER-negative advanced-stage breast cancer patients. Clin. Cancer Res., 2007-05-01;13(9):2714-21. 2007-05-01 [PMID: 17473204] (Flow Cytometry, Human)
• Tuyaerts S, Van Meirvenne S, Bonehill A, Heirman C, Corthals J, Waldmann H, Breckpot K, Thielemans K, Aerts JL Expression of human GITRL on myeloid dendritic cells enhances their immunostimulatory function but does not abrogate the suppressive effect of CD4+CD25+ regulatory T cells. J. Leukoc. Biol., 2007-04-20;82(1):93-105. 2007-04-20 [PMID: 17449724] (Flow Cytometry, Human)
• Yang XH, Yamagiwa S, Ichida T, Matsuda Y, Sugahara S, Watanabe H, Sato Y, Abo T, Horwitz DA, Aoyagi Y Increase of CD4+ CD25+ regulatory T-cells in the liver of patients with hepatocellular carcinoma. J. Hepatol., 2006-03-09;45(2):254-62. 2006-03-09 [PMID: 16600416] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: TNFRSF18
• Uniprot:
  • Q9Y5U5()