Ghrelin/Obestatin Antibody

Images

 
Immunohistochemistry-Paraffin: Ghrelin Antibody [NB600-813] - Ghrelin preproprotein Antibody [NB600-813] - IHC staining of paraffin embedded Human Placenta using this antibody at 2ug/ml. Steamed antigen retrieval with ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Ghrelin/Obestatin Antibody Summary

Immunogen
Peptide with sequence C-QDILWEEAKEAPADK corresponding to C-Terminus according to NP_057446.1, NP_001128413.1, NP_001128416.1, NP_001128417.1, NP_001128418.1.
Specificity
This antibody is expected to recognise all reported isoforms.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GHRL
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 2 - 5 ug/ml
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Peptide ELISA Detection limit 1:8000
Application Notes
ICC successful usage in ICC for rat neurons is reported in scientific literature (PMID: 19540881). WB: Not yet tested - Our standard WB protocol would not expect to reliably detect proteins as small as the calculated size of this preproprotein of approx. 12.9 kDa band according to NP_057446.1 and of its derivatives. IHC-P: Human placenta shows staining of trophoblast cytoplasm.
Positive Control
Ghrelin/Obestatin Lysate (NBL1-11067)
Publications
Read Publications using NB600-813.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ghrelin/Obestatin Antibody

  • appetite-regulating hormone
  • ghrelin
  • ghrelin/obestatin preprohormone
  • ghrelin/obestatin prepropeptide
  • Ghrelin/Obestatin
  • Growth hormone secretagogue
  • Growth hormone-releasing peptide
  • Motilin-related peptide
  • MTLRPgrowth hormone secretagogue receptor ligand
  • obestatin
  • Protein M46

Background

Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ghrelin/Obestatin Antibody (NB600-813)(2)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GHRL
Entrez
Uniprot