Bax inhibitor 1 Antibody (20F430)

Immunohistochemistry-Paraffin: Bax inhibitor 1 Antibody (20F430) [NBP2-24919] - Formalin-fixed, paraffin-embedded tissue sections stained for Bi-1 expression using this antibody at 5 ug/ml. A: normal human lung alveoli with positive lung macrophages. B: normal mouse cartilage with positive chondrocytes. Hematoxylin-eosin counterstain.

Immunohistochemistry-Paraffin: Bax inhibitor 1 Antibody (20F430) [NBP2-24919] - Formalin-fixed, paraffin-embedded human adenocarcinoma of the rectum stained with Bi-1 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Immunohistochemistry-Paraffin: Bax inhibitor 1 Antibody (20F430) [NBP2-24919] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human liver cancer using 5 ug/ml concentration of Bax inhibitor 1 antibody (clone 20F430). Intense cytoplasmic immune-positivity of Bax inhibitor 1 (TMBIM6) was observed in the hepatis cancer cells [10X Magnification]

Immunohistochemistry-Paraffin: Bax inhibitor 1 Antibody (20F430) [NBP2-24919] - Formalin-fixed, paraffin-embedded normal human tissue sections stained for Bi-1 expression using this antibody at 5 ug/ml. A: liver. B: skin. Hematoxylin-eosin counterstain.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 20F430
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Bax inhibitor 1 Antibody (20F430) Summary

• Immunogen: A synthetic peptide made to the N-terminal portion of the human Bax inhibitor 1 protein (between amino acids 1-40) UniProt P55061.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TMBIM6
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot reported in scientific literature (PMID 20359348)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.2% Gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Bax inhibitor 1 Antibody (20F430)

• BAXI1
• BI1
• BI-1Transmembrane BAX inhibitor motif-containing protein 6
• TEGTbax inhibitor 1
• testis enhanced gene transcript
• Testis-enhanced gene transcript protein
• transmembrane BAX inhibitor motif containing 6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Bax inhibitor 1 Antibody (NBP2-24919)(2)

Publications using NBP2-24919

• Kotsafti A, Farinati F, Cardin R et al. Bax inhibitor-1 down-regulation in the progression of chronic liver diseases. BMC Gastroenterol. 2010-04-01 [PMID: 20359348] (WB)
  
  Details:
  Bi-1 (IMG-5796). WB: Cirrhotic non-cancerious liver tissues (PHCC) and in surrounding hepatocellular carcinoma (HCC) tissues obtained from the same patient, Fig 3A.
• Zhang M, Li X, Zhang Y, Zhou K. Bax inhibitor-1 mediates apoptosis-resistance in human nasopharyngeal carcinoma cells. Mol Cell Biochem. 2010-01-01 [PMID: 19579059] (WB, Human)
  
  Details:
  Product cited: Bi-1 (IMG-5796). WB: CNE-2Z (poorly differentiated human nasopharyngeal carcinoma cell line) and CNE-1 (well differentiated human nasopharyngeal carcinoma cell line) cell line, Fig 1A. WB: Parental and transfected Bi-1 siRNA CNE-2Z and CNE-

Bioinformatics

• Gene Symbol: TMBIM6
• Uniprot:
  • P55061(Human)