DDX1 Antibody (3E5E2) - BSA Free

Western blot shows lysates of U2OS human osteosarcoma parental cell line and DDX1 knockout U2OS cell line (KO). Nitrocellulose membrane was probed with DDX1 Antibody (3E5E2) - BSA Free (Catalog # NBP2-61745) followed by HRP-conjugated secondary antibody. A specific band was detected for DDX1 at approximately 82.4 kDa (as indicated) in the parental U2OS cell line, but is not detectable in knockout U2OS cell line. Primary antibody dilution used: 1/500. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

U2OS human osteosarcoma cell line lysates were prepared and immunoprecipitation was performed using 2.0 μg of DDX1 Antibody (3E5E2) - BSA Free (Catalog # NBP2-61745) pre-coupled to Dynabeads Protein G. Immunoprecipitated DDX1 was detected in Western Blot with a rabbit DDX1 antibody used at 1/1000. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Western Blot: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis using DDX1 mAb against HEK293 (1) and DDX1 (AA: 642-740)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of Hela cells using DDX1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry-Paraffin: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of bladder cancer tissues using DDX1 mouse mAb with DAB staining.

Flow Cytometry: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of Hela cells using DDX1 mouse mAb (green) and negative control (red).

Western Blot: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis using DDX1 mAb against human DDX1 (AA: 642-740) recombinant protein. (Expected MW is 37 kDa)

Western Blot: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis using DDX1 mouse mAb against Hela (1), MCF-7 (2), A431 (3), PC-3 (4), NIH/3T3 (5), Jurkat (6), U251 (7), and HEK293 (8) cell lysate.

Immunohistochemistry-Paraffin: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of colon cancer tissues using DDX1 mouse mAb with DAB staining.

ELISA: DDX1 Antibody (3E5E2) [NBP2-61745] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP, KO
• Clone: 3E5E2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

DDX1 Antibody (3E5E2) - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Mouse DDX1 Antibody (3E5E2) - BSA Free (NBP2-61745) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow, ICC/IF and IP. Anti-DDX1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human DDX1 (AA: 642-740) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DDX1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation
  • Knockout Validated Validated for Knockout from YCharOS Inc. (ycharos.com)
  • Western Blot 1:500-1:2000
• Application Notes: Use in Immunoprecipitation reported in scientific literature (PMID:32755435)
• Theoretical MW: 82.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for DDX1 Antibody (3E5E2) - BSA Free

• ATP-dependent RNA helicase DDX1
• DBP-RB
• DBP-RBDEAD box polypeptide 1
• DDX1
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
• DEAD box protein 1
• DEAD box protein retinoblastoma
• DEAD Box-1
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1
• DEAD/H-Box Helicase 1
• EC 3.6.1
• EC 3.6.1.10
• EC 3.6.4.13
• UKVH5d

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DDX1 Antibody (NBP2-61745)(2)

Publications using NBP2-61745

• Lu S, Ye Q, Singh D, et al. The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein Nature communications 2021-01-21 [PMID: 33479198] (ICC/IF, Human)
• Sharma M, Wente SR Nucleocytoplasmic shuttling of Gle1 impacts DDX1 at transcription termination sites Mol. Biol. Cell 2020-08-05 [PMID: 32755435] (IP, ICC/IF, WB, Human)

Review for DDX1 Antibody (NBP2-61745) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 05/07/2020

Summary

• Application: Western Blot
• Sample Tested: AC16
• Species: Human
• Lot: 160325

Bioinformatics

• Gene Symbol: DDX1