DDX1 Antibody (3E5E2) - BSA Free

Images

 
Western Blot: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis using DDX1 mAb against HEK293 (1) and DDX1 (AA: 642-740)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of Hela cells using DDX1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa ...read more
Immunohistochemistry-Paraffin: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of bladder cancer tissues using DDX1 mouse mAb with DAB staining.
Flow Cytometry: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of Hela cells using DDX1 mouse mAb (green) and negative control (red).
Western Blot: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis using DDX1 mAb against human DDX1 (AA: 642-740) recombinant protein. (Expected MW is 37 kDa)
Western Blot: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis using DDX1 mouse mAb against Hela (1), MCF-7 (2), A431 (3), PC-3 (4), NIH/3T3 (5), Jurkat (6), U251 (7), and HEK293 (8) cell lysate.
Immunohistochemistry-Paraffin: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of colon cancer tissues using DDX1 mouse mAb with DAB staining.
ELISA: DDX1 Antibody (3E5E2) [NBP2-61745] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP
Clone
3E5E2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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Catalog# & Formulation Size Price

DDX1 Antibody (3E5E2) - BSA Free Summary

Immunogen
Purified recombinant fragment of human DDX1 (AA: 642-740) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DDX1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation
  • Western Blot 1:500-1:2000
Application Notes
Use in Immunoprecipitation reported in scientific literature (PMID:32755435)
Theoretical MW
82.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-61745 in the following applications:

Publications
Read Publications using
NBP2-61745 in the following applications:

  • 2 publications
  • IP
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for DDX1 Antibody (3E5E2) - BSA Free

  • ATP-dependent RNA helicase DDX1
  • DBP-RB
  • DBP-RBDEAD box polypeptide 1
  • DDX1
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
  • DEAD box protein 1
  • DEAD box protein retinoblastoma
  • DEAD Box-1
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1
  • DEAD/H-Box Helicase 1
  • EC 3.6.1
  • EC 3.6.1.10
  • EC 3.6.4.13
  • UKVH5d

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DDX1 Antibody (NBP2-61745)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IP, WB.


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ICC/IF
(2)
IP
(1)
WB
(1)
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Human
(2)
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Review for DDX1 Antibody (NBP2-61745) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-61745:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot DDX1 NBP2-61745
Enlarge
5
reviewed by:
Verified Customer
WB Human 05/07/2020
View

Summary

ApplicationWestern Blot
Sample TestedAC16
SpeciesHuman
Lot160325

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DDX1 Antibody (NBP2-61745) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
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2
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1
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Verified Customer
05/07/2020
Application: WB
Species: Human

Bioinformatics

Gene Symbol DDX1