GFR alpha-3/GDNF R alpha-3 Antibody (111004)

Images

 
Western blot shows lysates of human stomach tissue and human small intestine tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GFR alpha ‑3/GDNF R alpha ‑3 Monoclonal Antibody (Catalog # MAB6701) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
111004
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price

GFR alpha-3/GDNF R alpha-3 Antibody (111004) Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human GFR alpha ‑3/GDNF R alpha ‑3
Gly31-Trp382
Accession # AAC24355
Specificity
Detects human GFR alpha ‑3/GDNF R alpha ‑3 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) GFR alpha -3 is observed and no cross-reactivity with recombinant human (rh) GFR alpha -1 or rhGFR alpha -4 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GFRA3
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GFR alpha-3/GDNF R alpha-3 Antibody (111004)

  • GDNF family receptor alpha 3
  • GDNF family receptor alpha-3
  • GDNF R alpha-3
  • GDNF receptor alpha-3
  • GDNFR-alpha-3
  • GFR alpha3
  • GFR alpha-3
  • GFRA3
  • GFRa-3
  • GFR-alpha-3
  • glial cell line-derived neurotrophic factor receptor alpha-3
  • GPI-linked receptor

Background

Glial cell line-derived growth factor (GDNF), neurturin (NTN), persephin (PEP) and artemin (ART), distant members of the TGF-beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of these neurotrophic factors are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and one of four ligand binding cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins (GFR alpha -1, 2, 3, or 4). These four GFR-alpha  proteins share conserved placements of many of their cysteine residues. GFR alpha -1, 2, 3, or 4 have been shown to preferentially bind GDNF, NTN, ART, and PSP, respectively. While the GFR alpha -3/Ret complex is highly specific for ART, the GFR alpha -1 /Ret complex is extremely promiscuous and may also be utilized by NTN and ART. Human GFR alpha -3 cDNA encodes a 400 amino acid (aa) precursor protein with an N-terminal signal peptide and C-terminal hydrophobic domain. GFR alpha -3 is approximately 34% and 36% identical to GFR alpha -1 and GFR alpha -2, respectively. Human and mouse GFR alpha -3 share approximately 76% aa sequence homology. The gene encoding GFR alpha -3 has been localized to human chromosome 5. GFR alpha -3 is expressed at high levels in the developing and adult sensory and sympathetic ganglia of the peripheral nervous system. It is also expressed in non-neural tissues and may show tissue-specific differences in molecular weight. In the trigeminal ganglions, the expression of GFR alpha -3 is found in a population of neurons distinct from those expressing GFR alpha -1 or 2.

  1. Baloh, R. et al. (1998) Neuron 21:1291.
  2. Baloh, R. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
  3. Worby, C. et al. (1998) J. Biol. Chem. 273:3502.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

212-GD/CF
Species: Hu
Applications: Bind, BA
AF1085
Species: Mu
Applications: IHC, Neut, WB
AF482
Species: Mu
Applications: IHC, WB
1297-NE/CF
Species: Hu
Applications: Bind, BA
AF429
Species: Hu, Mu
Applications: Block, ICC, IHC, WB
267-N3/CF
Species: Hu
Applications: BA
NBP2-35208
Species: Hu
Applications: BA, Func, PAGE
NBP2-57362
Species: Hu
Applications: ICC/IF, WB
DBD00
Species: Hu
Applications: ELISA
NBP2-24653
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP2-02541
Species: Hu, Pm, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF1056
Species: Rt
Applications: IHC, WB
NB100-1617
Species: Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
DUP00
Species: Hu
Applications: ELISA
MAB6701
Species: Hu
Applications: WB

Publications for GFR alpha-3/GDNF R alpha-3 Antibody (MAB6701) (0)

There are no publications for GFR alpha-3/GDNF R alpha-3 Antibody (MAB6701).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GFR alpha-3/GDNF R alpha-3 Antibody (MAB6701) (0)

There are no reviews for GFR alpha-3/GDNF R alpha-3 Antibody (MAB6701). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GFR alpha-3/GDNF R alpha-3 Antibody (MAB6701) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional GFR alpha-3/GDNF R alpha-3 Products

Bioinformatics Tool for GFR alpha-3/GDNF R alpha-3 Antibody (MAB6701)

Discover related pathways, diseases and genes to GFR alpha-3/GDNF R alpha-3 Antibody (MAB6701). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GFR alpha-3/GDNF R alpha-3 Antibody (MAB6701)

Discover more about diseases related to GFR alpha-3/GDNF R alpha-3 Antibody (MAB6701).
 

Pathways for GFR alpha-3/GDNF R alpha-3 Antibody (MAB6701)

View related products by pathway.

PTMs for GFR alpha-3/GDNF R alpha-3 Antibody (MAB6701)

Learn more about PTMs related to GFR alpha-3/GDNF R alpha-3 Antibody (MAB6701).

Blogs on GFR alpha-3/GDNF R alpha-3

There are no specific blogs for GFR alpha-3/GDNF R alpha-3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GFR alpha-3/GDNF R alpha-3 Antibody (111004) and receive a gift card or discount.

Bioinformatics

Gene Symbol GFRA3
Uniprot