GDNF Antibody

GDNF and GFR alpha ‑1 were detected in perfusion fixed frozen sections of rat dorsal root ganglia using 10 µg/mL Goat Anti-Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF-212-NA) and 15 µg/mL Biotinylated Goat Anti-Rat GFR alpha ‑1 Antigen Affinity-purified Polyclonal Antibody (red; Catalog # BAF560). Tissue was incubated with primary antibodies overnight at 4 °C. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Recombinant Human GDNF (Catalog # 212-GD) induces proliferation in the SH‑SY5Y human neuroblastoma cell line in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714-GR) in a dose-dependent manner (orange line), as measured by Rezazurin (Catalog # AR002). Under these conditions, proliferation elicited by GDNF is neutralized (green line) by increasing concentrations of Goat Anti-Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-212-NA). The ND50 is typically 0.25-1 μg/mL.

• Reactivity: Hu, Rt
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

GDNF Antibody Summary

• Immunogen: E. coli-derived and Mouse myeloma cell line NS0-derived recombinant human GDNF
  Arg109-Ile211
  Accession # P39905
• Specificity: Detects human and rat GDNF in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GDNF
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.25-1 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GDNF Antibody

• Astrocyte-derived trophic factor
• ATF
• ATF1
• ATF2
• GDNF
• glial cell derived neurotrophic factor
• glial cell line derived neurotrophic factor
• glial cell line-derived neurotrophic factor
• HFB1-GDNF
• HGDNF
• HSCR3

Background

Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons. Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell-expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GDNF Antibody (AF-212-NA)(48)

Publications using AF-212-NA

• Ford, MM;George, BE;Van Laar, VS;Holleran, KM;Naidoo, J;Hadaczek, P;Vanderhooft, LE;Peck, EG;Dawes, MH;Ohno, K;Bringas, J;McBride, JL;Samaranch, L;Forsayeth, JR;Jones, SR;Grant, KA;Bankiewicz, KS; GDNF gene therapy for alcohol use disorder in male non-human primates Nature medicine 2023-08-01 [PMID: 37580533] (IHC, Primate - Rhesus macaque)
• Yuki Saito, Maki Miyajima, Sena Yamamoto, Norihiro Miura, Tsukasa Sato, Arisa Kita et al. Intrathecal Injection of Mesenchymal Stromal Cell Cultured on 3D Fiber Ameliorates Multiple Organ Damage in Murine Lupus Stem Cells Translational Medicine 6/22/2022 [PMID: 35466994]
• N Sultan, BA Scheven Exploring the potential application of dental pulp stem cells in neuroregenerative medicine Neural regeneration research, 2022-04-01;17(4):775-776. 2022-04-01 [PMID: 34472466] (Neutralization, Rat)
• N Sultan, LE Amin, AR Zaher, ME Grawish, BA Scheven Dental pulp stem cells stimulate neuronal differentiation of PC12 cells Neural regeneration research, 2021-09-01;16(9):1821-1828. 2021-09-01 [PMID: 33510089] (Neutralization, Rat)
• J Yin, HM Chang, Y Yi, Y Yao, PCK Leung TGF-beta1 Increases GDNF Production by Upregulating the Expression of GDNF and Furin in Human Granulosa-Lutein Cells Cells, 2020-01-10;9(1):. 2020-01-10 [PMID: 31936902] (Western Blot, Human)
• James W. H. Sonne, Jason S. Groshong, Corey Seavey, Don M. Gash Spatial and temporal immunoreactivity in the rat brain using an affinity purified polyclonal antibody to DNSP-11 Journal of Chemical Neuroanatomy 10/1/2019 [PMID: 31394198]
• Dwaine F. Emerich, Jeffrey H. Kordower, Yaping Chu, Chris Thanos, Briannan Bintz, Giovanna Paolone et al. Widespread Striatal Delivery of GDNF from Encapsulated Cells Prevents the Anatomical and Functional Consequences of Excitotoxicity Neural Plasticity 3/11/2019 [PMID: 30984255]
• Masao Nakasatomi, Shunsuke Takahashi, Toru Sakairi, Hidekazu Ikeuchi, Yoriaki Kaneko, Keiju Hiromura et al. Enhancement of HGF-induced tubulogenesis by endothelial cell-derived GDNF PLOS ONE 3/7/2019 [PMID: 30845150]
• L Quintino, A Namislo, M Davidsson, LS Breger, P Kavanagh, M Avallone, E Elgstrand-, C Isaksson, C Lundberg Destabilizing Domains Enable Long-Term and Inert Regulation of GDNF Expression in the Brain Mol Ther Methods Clin Dev, 2018-09-04;11(0):29-39. 2018-09-04 [PMID: 30324128] (IHC, Rat)
• Anna-Maija Penttinen, Ilmari Parkkinen, Merja H. Voutilainen, Maryna Koskela, Susanne Bäck, Anna Their et al. Pre-alpha -pro-GDNF and Pre-beta -pro-GDNF Isoforms Are Neuroprotective in the 6-hydroxydopamine Rat Model of Parkinson's Disease Frontiers in Neurology 6/20/2018 [PMID: 29973907]
• R Jalili, J Horecka, JR Swartz, RW Davis, HHJ Persson Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies Proc. Natl. Acad. Sci. U.S.A., 2018-01-16;0(0):. 2018-01-16 [PMID: 29339495] (Affinity Assay)
• Richard Mills, Hermes Taylor-Weiner, Jorge C. Correia, Leandro Z. Agudelo, Ilary Allodi, Christina Kolonelou et al. Neurturin is a PGC-1 alpha 1-controlled myokine that promotes motor neuron recruitment and neuromuscular junction formation Molecular Metabolism 1/1/2018 [PMID: 29157948]
• K Nagashima, T Miwa, H Soumiya, D Ushiro, T Takeda-Kaw, N Tamaoki, S Ishiguro, Y Sato, K Miyamoto, T Ohno, M Osawa, T Kunisada, T Shibata, KI Tezuka, S Furukawa, H Fukumitsu Priming with FGF2 stimulates human dental pulp cells to promote axonal regeneration and locomotor function recovery after spinal cord injury Sci Rep, 2017-10-18;7(1):13500. 2017-10-18 [PMID: 29044129] (ICC, Human)
• J Lavaur, D Le Nogue, M Lemaire, J Pype, G Farjot, EC Hirsch, PP Michel The Noble Gas Xenon Provides Protection and Trophic Stimulation to Midbrain Dopamine Neurons J. Neurochem., 2017-05-16;0(0):. 2017-05-16 [PMID: 28398653] (Neutralization, Rat)
• Y Dong, A Kanda, K Noda, W Saito, S Ishida Pathologic Roles of Receptor-Associated Prorenin System in Idiopathic Epiretinal Membrane Sci Rep, 2017-03-09;7(0):44266. 2017-03-09 [PMID: 28276504] (IHC-P, Human)
• GM Thomsen, M Alkaslasi, JP Vit, G Lawless, M Godoy, G Gowing, O Shelest, CN Svendsen Systemic injection of AAV9-GDNF provides modest functional improvements in the SOD1(G93A) ALS rat but has adverse side effects Gene Ther, 2017-03-09;0(0):. 2017-03-09 [PMID: 28276446] (IHC, Rat)
• SR Stott, S Hayat, T Carnwath, S Garas, JP Sleeman, RA Barker CD24 expression does not affect dopamine neuronal survival in a mouse model of Parkinson&#039;s disease PLoS ONE, 2017-02-09;12(2):e0171748. 2017-02-09 [PMID: 28182766] (IHC, Mouse)
• Christopher R. Hansebout, Caixin Su, Kiran Reddy, Donald Zhang, Cai Jiang, Michel P. Rathbone et al. Enteric glia mediate neuronal outgrowth through release of neurotrophic factors Neural Regen Res 10/5/2012 [PMID: 25538736]
• Wang T, Bruggeman K, Sheean R, Turner B, Nisbet D, Parish C Characterization of the stability and bio-functionality of tethered proteins on bioengineered scaffolds: implications for stem cell biology and tissue repair. J Biol Chem, 2014-04-03;289(21):15044-51. 2014-04-03 [PMID: 24700461] (ELISA Development (Detection), N/A)
• Sarin S, Boivin F, Li A, Lim J, Svajger B, Rosenblum N, Bridgewater D beta-Catenin overexpression in the metanephric mesenchyme leads to renal dysplasia genesis via cell-autonomous and non-cell-autonomous mechanisms. Am J Pathol, 2014-03-15;184(5):1395-410. 2014-03-15 [PMID: 24637293] (IHC-P, Mouse)
• Quintino L, Manfre G, Wettergren E, Namislo A, Isaksson C, Lundberg C Functional neuroprotection and efficient regulation of GDNF using destabilizing domains in a rodent model of Parkinson&#039;s disease. Mol Ther, 2013-07-24;21(12):2169-80. 2013-07-24 [PMID: 23881415] (IHC-Fr, Western Blot, Human, Rat)
• Susanne Bäck, Johan Peränen, Emilia Galli, Päivi Pulkkila, Liina Lonka-Nevalaita, Tuulia Tamminen et al. Gene therapy with AAV2-CDNF provides functional benefits in a rat model of Parkinson's disease Brain and Behavior 3/1/2013 [PMID: 23532969]
• Taylor H, Barua N, Bienemann A, Wyatt M, Castrique E, Foster R, Luz M, Fibiger C, Mohr E, Gill S Clearance and toxicity of recombinant methionyl human glial cell line-derived neurotrophic factor (r-metHu GDNF) following acute convection-enhanced delivery into the striatum. PLoS ONE, 2013-03-20;8(3):e56186. 2013-03-20 [PMID: 23526931] (IHC, Rat)
• Somayeh Ahmadiantehrani, Dorit Ron Dopamine D2 receptor activation leads to an up-regulation of glial cell line-derived neurotrophic factor via G beta gamma -Erk1/2-dependent induction of Zif268 Journal of Neurochemistry 4/1/2013 [PMID: 23373701]
• Somayeh Ahmadiantehrani, Dorit Ron Dopamine D2 receptor activation leads to an up-regulation of glial cell line-derived neurotrophic factor via G beta gamma -Erk1/2-dependent induction of Zif268 Journal of Neurochemistry 2013-04-01 [PMID: 23373701] (Western Blot, Rat)
• Ohshima-Hosoyama S, Simmons HA, Goecks N A Monoclonal Antibody-GDNF Fusion Protein Is Not Neuroprotective and Is Associated with Proliferative Pancreatic Lesions in Parkinsonian Monkeys. PLoS ONE, 2012-06-20;7(6):e39036. 2012-06-20 [PMID: 22745701] (IHC-Fr, Primate - Macaca mulatta (Rhesus Macaque))
• Bonanomi D, Chivatakarn O, Bai G, Abdesselem H, Lettieri K, Marquardt T, Pierchala BA, Pfaff SL. Ret Is a Multifunctional Coreceptor that Integrates Diffusible- and Contact-Axon Guidance Signals. Cell;148(3):568-82. 2/3/2012 [PMID: 22304922]
• Valentina Di Liberto, Giuseppa Mudò, Natale Belluardo mGluR2/3 agonist LY379268, by enhancing the production of GDNF, induces a time-related phosphorylation of RET receptor and intracellular signaling Erk1/2 in mouse striatum Neuropharmacology 9/1/2011 [PMID: 21619889]
• Richardson RM, Kells AP, Rosenbluth KH, Salegio EA, Fiandaca MS, Larson PS, Starr PA, Martin AJ, Lonser RR, Federoff HJ, Forsayeth JR, Bankiewicz KS Interventional MRI-guided putaminal delivery of AAV2-GDNF for a planned clinical trial in Parkinson&#039;s disease. Mol. Ther., 2011-02-22;19(6):1048-57. 2011-02-22 [PMID: 21343917] (IHC-Fr, Primate - Macaca mulatta (Rhesus Macaque))
• Ann D. Cohen, Michael J. Zigmond, Amanda D. Smith Effects of intrastriatal GDNF on the response of dopamine neurons to 6-hydroxydopamine: Time course of protection and neurorestoration Brain Research 1/25/2011 [PMID: 21062624]
• Hadaczek P, Johnston L, Forsayeth J, Bankiewicz KS Pharmacokinetics and bioactivity of glial cell line-derived factor (GDNF) and neurturin (NTN) infused into the rat brain. Neuropharmacology, 2010-02-11;58(7):1114-21. 2010-02-11 [PMID: 20153340] (IHC-Fr, Rat)
• Hongtao Wang, Inna Hughes, William Planer, Alexander Parsadanian, John R. Grider, Bhupinder P. S. Vohra et al. The Timing and Location of Glial Cell Line-Derived Neurotrophic Factor Expression Determine Enteric Nervous System Structure and Function The Journal of Neuroscience 1/27/2010 [PMID: 20107080]
• Hongtao Wang, Inna Hughes, William Planer, Alexander Parsadanian, John R. Grider, Bhupinder P. S. Vohra et al. The Timing and Location of Glial Cell Line-Derived Neurotrophic Factor Expression Determine Enteric Nervous System Structure and Function The Journal of Neuroscience 2010-01-27 [PMID: 20107080] (Immunohistochemistry, Transgenic Mouse)
• Emborg ME, Moirano J, Raschke J, Bondarenko V, Zufferey R, Peng S, Ebert AD, Joers V, Roitberg B, Holden JE, Koprich J, Lipton J, Kordower JH, Aebischer P Response of aged parkinsonian monkeys to in vivo gene transfer of GDNF. Neurobiol. Dis., 2009-08-04;36(2):303-11. 2009-08-04 [PMID: 19660547] (IHC-Fr, Primate - Macaca mulatta (Rhesus Macaque))
• Kells AP, Hadaczek P, Yin D, Bringas J, Varenika V, Forsayeth J, Bankiewicz KS Efficient gene therapy-based method for the delivery of therapeutics to primate cortex. Proc. Natl. Acad. Sci. U.S.A., 2009-02-04;106(7):2407-11. 2009-02-04 [PMID: 19193857] (IHC, Primate - Macaca mulatta (Rhesus Macaque))
• Suzuki M, McHugh J, Tork C, Shelley B, Hayes A, Bellantuono I, Aebischer P, Svendsen CN Direct muscle delivery of GDNF with human mesenchymal stem cells improves motor neuron survival and function in a rat model of familial ALS. Mol. Ther., 2008-09-16;16(12):2002-10. 2008-09-16 [PMID: 18797452] (IHC-Fr, Human)
• Ai X, Kitazawa T, Do AT, Kusche-Gullberg M, Labosky PA, Emerson CP SULF1 and SULF2 regulate heparan sulfate-mediated GDNF signaling for esophageal innervation. Development, 2007-09-01;134(18):3327-38. 2007-09-01 [PMID: 17720696] (IHC-Fr, Mouse)
• Hamra FK, Chapman KM, Nguyen D, Garbers DL Identification of neuregulin as a factor required for formation of aligned spermatogonia. J. Biol. Chem., 2006-11-10;282(1):721-30. 2006-11-10 [PMID: 17098736] (Neutralization, Rat)
• Xu L, Yan J, Chen D, Welsh AM, Hazel T, Johe K, Hatfield G, Koliatsos VE Human neural stem cell grafts ameliorate motor neuron disease in SOD-1 transgenic rats. Transplantation, 2006-10-15;82(7):865-75. 2006-10-15 [PMID: 17038899] (IHC, Rat)
• Kobayashi T, Ahlenius H, Thored P, Kobayashi R, Kokaia Z, Lindvall O Intracerebral infusion of glial cell line-derived neurotrophic factor promotes striatal neurogenesis after stroke in adult rats. Stroke, 2006-07-27;37(9):2361-7. 2006-07-27 [PMID: 16873711] (IHC-Fr, Rat)
• Fouchecourt S, Godet M, Sabido O, Durand P Glial cell-line-derived neurotropic factor and its receptors are expressed by germinal and somatic cells of the rat testis. J. Endocrinol., 2006-07-01;190(1):59-71. 2006-07-01 [PMID: 16837611] (Flow Cytometry, ICC, Western Blot, Rat)
• Oo TF, Ries V, Cho J, Kholodilov N, Burke RE Anatomical basis of glial cell line-derived neurotrophic factor expression in the striatum and related basal ganglia during postnatal development of the rat. J. Comp. Neurol., 2005-03-28;484(1):57-67. 2005-03-28 [PMID: 15717300] (ICC, IHC-Fr, Rat)
• Esper RM, Loeb JA Rapid axoglial signaling mediated by neuregulin and neurotrophic factors. J. Neurosci., 2004-07-07;24(27):6218-27. 2004-07-07 [PMID: 15240814] (Neutralization, Chicken)
• Benner EJ, Mosley RL, Destache CJ, Lewis TB, Jackson-Lewis V, Gorantla S, Nemachek C, Green SR, Przedborski S, Gendelman HE Therapeutic immunization protects dopaminergic neurons in a mouse model of Parkinson's disease. Proc. Natl. Acad. Sci. U.S.A., 2004-06-14;101(25):9435-40. 2004-06-14 [PMID: 15197276] (IHC, Mouse)
• Sun W, Oppenheim RW Response of motoneurons to neonatal sciatic nerve axotomy in Bax-knockout mice. Mol. Cell. Neurosci., 2003-12-01;24(4):875-86. 2003-12-01 [PMID: 14697655] (Neutralization, Mouse)
• Eslamboli A, Cummings RM, Ridley RM, Baker HF, Muzyczka N, Burger C, Mandel RJ, Kirik D, Annett LE Recombinant adeno-associated viral vector (rAAV) delivery of GDNF provides protection against 6-OHDA lesion in the common marmoset monkey (Callithrix jacchus). Exp. Neurol., 2003-11-01;184(1):536-48. 2003-11-01 [PMID: 14637123] (IHC, Primate - Callitrix jacchus (Common Marmoset))
• Honma M, Namikawa K, Mansur K, Iwata T, Mori N, Iizuka H, Kiyama H Developmental alteration of nerve injury induced glial cell line-derived neurotrophic factor (GDNF) receptor expression is crucial for the determination of injured motoneuron fate. J. Neurochem., 2002-08-01;82(4):961-75. 2002-08-01 [PMID: 12358802] (IHC, Rat)
• Pina Serra M, 2019, Quartu M, Ambu R, Follesa P, Del Fiacco M Immunohistochemical localization of GDNF in the human hippocampal formation from prenatal life to adulthood. 6811, 2002-02-22;928(1):138-46. 2002-02-22 [PMID: 11844480] (IHC-Fr, Western Blot, Human, Rat)

Reviews for GDNF Antibody (AF-212-NA) (3)

Average Rating: 4.3

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Human.

reviewed by: Verified Customer IF Human 12/18/2014

Summary

• Application: Immunofluorescence
• Sample Tested: Human GDNF bound to the surface of HEK293 cells transfected with the GDNF receptor GFRa1
• Species: Human

reviewed by: Verified Customer IP Human 12/18/2014

Summary

• Application: Immunoprecipitation
• Sample Tested: N/A
• Species: Human

reviewed by: Verified Customer WB Human 12/18/2014

Summary

• Application: Western Blot
• Sample Tested: Recombinant GDNF binding to 293 cells expressing GDNF receptors or primary hippocampal neurons or brain homogenates
• Species: Human

Bioinformatics

• Gene Symbol: GDNF
• Uniprot:
  • P39905(Goat)