Recombinant Mouse Artemin Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity, Bioactivity

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Recombinant Mouse Artemin Protein Summary

Details of Functionality
Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is 2-8 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse GFR alpha ‑3/GDNF R alpha ‑3 Fc Chimera (Catalog # 2645-FR) at 1 µg/mL (100 µL/well) can bind Recombinant Mouse Artemin with an apparent KD <1 nM.
Source
E. coli-derived mouse Artemin protein
Ala112-Gly224
Accession #
N-terminal Sequence
Ala112
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Artn
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
12 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
1085-AR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4 mM HCI containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Artemin Protein

  • Artemin
  • ARTN
  • Enovin
  • EVN
  • EVNneurotrophic factor
  • NBN
  • Neublastin

Background

Artemin is a member of the Glia Cell-Derived Neurotrophic factor (GDNF) family ligands, which include GDNF, Persephin, Artemin, and Neurturin. GDNF family ligands are distant members of the Transforming Growth Factor beta (TGF-beta ) superfamily (1-4). Similar to other TGF-beta family proteins, Artemin is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of Artemin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine-knot and the single interchain disulfide bond. Biologically active Artemin is a disulfide-linked homodimer of the carboxy-terminal 113 amino acid residues. Mature mouse Artemin shares 88.5% amino acid sequence similarity with human Artemin. Mature Artemin also shares approximately 40% amino acid sequence identity with the other three members of the GDNF family ligands (5). Bioactivities of all GDNF family ligands are mediated through a receptor complex composed of a high affinity ligand binding component (GFR alpha -1-GFR alpha -4) and a common signaling component, cRET (receptor tyrosine kinase) (5-8). Artemin prefers to bind to GFR alpha -3 and activites the GFR alpha -3-RET. However, in the presence of RET, it can bind to GFR alpha -1 as well (4, 5, 9). Artemin has been shown to promote the survival and growth of various peripheral and central neurons, including sympathetic and dopaminergic neurons. It may also play an important role in the development of sympathetic neurons and several organs (5, 10, 11).

  1. Lin, L-F.H. et al. (1993) Science 260:1130.
  2. Milbrandt, J. et al. (1998) Neuron 20:245.
  3. Kotzbauer, P.T. et al. (1996) Nature 384:467.
  4. Baloh, R.H. et al. (1998) Neuron 21:1291.
  5. Takahashi, M. (2001) Cytokine and Growth Factor Reviews 12:361.
  6. Baloh, R.H. et al. (1997) Neuron 18:793.
  7. Jing, S. et al. (1996) Cell 85:1113.
  8. Jing, S. et al. (1997) J Biol Chem 272:33111.
  9. Nishino, J. et al. (1999) Neuron 23:725.
  10. Enomoto, H. et al. (2001) Development 128:3963.
  11. Andres, R. et al. (2001) Development 128:3685.

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Publications for Artemin (1085-AR)(6)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: Bioassay, In Vivo.


Filter By Application
Bioassay
(3)
In Vivo
(3)
All Applications
Filter By Species
Mouse
(5)
Rat
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using 1085-AR Applications Species
S Schaller, D Buttigieg, A Alory, A Jacquier, M Barad, M Merchant, D Gentien, P de la Gran, G Haase Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons Proc. Natl. Acad. Sci. U.S.A, 2017;114(12):E2486-E2493. 2017 [PMID: 28270618] (Bioassay, Mouse) Bioassay Mouse
EK Lippoldt, S Ongun, GK Kusaka, DD McKemy Inflammatory and neuropathic cold allodynia are selectively mediated by the neurotrophic factor receptor GFR?3 Proc Natl Acad Sci USA, 2016;113(16):4506-11. 2016 [PMID: 27051069] (In Vivo, Mouse) In Vivo Mouse
Ikeda-Miyagawa Y, Kobayashi K, Yamanaka H, Okubo M, Wang S, Dai Y, Yagi H, Hirose M, Noguchi K Peripherally increased artemin is a key regulator of TRPA1/V1 expression in primary afferent neurons. Mol Pain, 2015;11(0):8. 2015 [PMID: 25889103] (In Vivo, Rat) In Vivo Rat
Bohorquez D, Samsa L, Roholt A, Medicetty S, Chandra R, Liddle R An enteroendocrine cell-enteric glia connection revealed by 3D electron microscopy. PLoS ONE, 2014;9(2):e89881. 2014 [PMID: 24587096] (Bioassay, Mouse) Bioassay Mouse
Li J, Klein C, Liang C, Rauch R, Kawamura K, Hsueh AJ Autocrine regulation of early embryonic development by the artemin-GFRA3 (GDNF family receptor-alpha 3) signaling system in mice. FEBS Lett., 2009;583(15):2479-85. 2009 [PMID: 19580811] (Bioassay, Mouse) Bioassay Mouse
Malin SA, Molliver DC, Koerber HR, Cornuet P, Frye R, Albers KM, Davis BM Glial cell line-derived neurotrophic factor family members sensitize nociceptors in vitro and produce thermal hyperalgesia in vivo. J. Neurosci., 2006;26(33):8588-99. 2006 [PMID: 16914685] (In Vivo, Mouse) In Vivo Mouse

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Bioinformatics

Gene Symbol Artn
Uniprot