GCKR Antibody

Western Blot: GCKR Antibody [NBP1-28910] - (0.1ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Mu, Rt, Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GCKR Antibody Summary

• Description: Novus Biologicals Goat GCKR Antibody (NBP1-28910) is a polyclonal antibody validated for use in WB and ELISA. Anti-GCKR Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence KRFQHVIETPEPGK -C corresponding to N-Terminus according to NP_001477.1.
• Predicted Species: Human (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GCKR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 70 kDa band observed in mouse and rat liver lysates (calculated MW of 68.7 kDa band according to human NP_001477.1 and of 68.9 kDa band according to rat NP_037252.1).
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GCKR Antibody

• FGQTL5
• GKRP
• glucokinase (hexokinase 4) regulator
• glucokinase (hexokinase 4) regulatory protein
• Glucokinase regulator
• glucokinase regulatory protein

Background

This gene encodes a protein belonging to the GCKR subfamily of the SIS (Sugar ISomerase) family of proteins. The gene product is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. This gene is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young (MODY). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GCKR Antibody (NBP1-28910)(2)

Publications using NBP1-28910

• Tomilov A, Bettaieb A, Kim K et al. Shc depletion stimulates brown fat activity in vivo and in vitro. Aging Cell. 2014-09-26 [PMID: 25257068]
• Kathiresan S, Melander O, Guiducci C et al. Six new loci associated with blood low-density lipoprotein cholesterol, high-density lipoprotein cholesterol or triglycerides in humans. Nat Genet 2008-02-01 [PMID: 18193044]

Bioinformatics

• Gene Symbol: GCKR
• Entrez:
  • 2646(Human)
  • 231103(Mouse)
  • 25658(Rat)
• Uniprot:
  • Q14397(Human)