GATA-4 Antibody (OTI9F9) - Azide and BSA Free

Western Blot: GATA-4 Antibody (OTI9F9) - Azide and BSA Free [NBP2-70797] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATA4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA4.

Immunocytochemistry/Immunofluorescence: GATA-4 Antibody (OTI9F9) - Azide and BSA Free [NBP2-70797] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GATA4.

Immunohistochemistry: GATA-4 Antibody (OTI9F9) - Azide and BSA Free [NBP2-70797] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-GATA4 mouse monoclonal antibody.

Flow Cytometry: GATA-4 Antibody (OTI9F9) - Azide and BSA Free [NBP2-70797] - Staining of HeLa cells using anti-GATA4 mouse monoclonal antibody.

Western Blot: GATA-4 Antibody (OTI9F9) - Azide and BSA Free [NBP2-70797] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GATA4 monoclonal antibody.

Immunohistochemistry: GATA-4 Antibody (OTI9F9) - Azide and BSA Free [NBP2-70797] - Staining of paraffin-embedded Human endometrium tissue using anti-GATA4 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI9F9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

GATA-4 Antibody (OTI9F9) - Azide and BSA Free Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 296-442 of human GATA4 (NP_002043) produced in E.coli.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: GATA4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200-500
• Theoretical MW: 44.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for GATA-4 Antibody (OTI9F9) - Azide and BSA Free

• GATA binding protein 4
• GATA4
• GATA-4
• GATA-binding factor 4
• GATA-binding protein 4
• MGC126629
• transcription factor GATA-4

Background

GATA4 is a 46 kDa member of the GATA family of zinc finger containing transcription factors that is involved in the development of cardiac hypertrophy and remodeling, and plays a critical role in regulating basal and agonist or stress induced gene expression in cardiac and smooth muscle cell types. GATA4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain. Serine 105 of GATA4 is phosphorylated in response to agonist stimulation through MEK 1 ERK1 / 2, and weakly through JNK or p38 MAPKs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GATA4