GATA-3 Antibody

Product Discontinued

GATA-3 Antibody Summary

• Immunogen: The antiserum was produced against synthesized peptide derived from human GATA3. The immunogen region is: Internal.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GATA3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+, pH 7.4), 0.15M NaCl and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GATA-3 Antibody

• 131320
• GATA binding protein 3
• GATA3
• GATA-3
• GATA-binding factor 3
• GATA-binding protein 3
• HDRMGC5199
• MGC2346
• MGC5445
• trans-acting T-cell-specific transcription factor GATA-3

Background

GATA3 is a 45-50 kDa transcription factor found in the nucleus of T cells, mast cells, and embryonic brain and kidney that contains a GATA-type zinc finger motif. This transcription factor recognizes and binds the GATA core nucleotide motif (A/T) GATA (A/G) and has been shown to regulate T(H1) and T(H2) cell differentiation by directly binding to the IL-5 and IL-13 promoters, remodeling chromatin structure to open the IL-4 locus, and repressing IL-12R beta-2 chain expression. GATA3 has been shown to be induced by IL-4 through Stat6 and can be upregulated by positive feedback. GATA-3 can be modified by acetylation and has been shown to interact with Smad3. The Poly6071 antibody has been shown to be useful for Western blotting of human GATA3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GATA3
• Entrez:
  • 2625(Human)
  • 14462(Mouse)
• Uniprot:
  • P23771()