GAT-1/SLC6A1 Antibody - Azide Free

Western Blot: GAT-1/SLC6A1 Antibody [NB100-61097] - Rat hippocampal homogenate showing specific immunolabeling of the~ 67k GAT-1 protein.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

GAT-1/SLC6A1 Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit GAT-1/SLC6A1 Antibody - Azide Free (NB100-61097) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. Accession # P23978
• Specificity: Specific for endogenous levels of the ~67 kDa GAT-1/SLC6A1 protein. Immunolabeling is blocked by pre-adsorption of antibody with the peptide used to generate the antibody.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC6A1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:1000
• Theoretical MW: 67 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

It is anticipated that the antibody will also react with mouse tissue based on the fact that this species has 100% homology with the amino acid sequence used as antigen.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Affinity purified

Alternate Names for GAT-1/SLC6A1 Antibody - Azide Free

• GABA Transporter 1
• GABATHG
• GABATR
• GABATRGAT-1
• GABT1
• GAT1
• GAT-1
• GAT1sodium- and chloride-dependent GABA transporter 1
• SLC6A1
• solute carrier family 6 (neurotransmitter transporter, GABA), member 1
• Solute carrier family 6 member 1

Background

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl negative channel associated with the GABAA receptor (GABAA-R) subtype. GABA plasma membrane transporters (GATs) influence synaptic neurotransmission by high-affinity uptake and release of GABA. To date, four distinct GABA transporters have been identified: GAT-1, GAT-2, GAT-3, and BGT-1. GAT-1, the most abundant of the transporters, is found predominantly in neurons, but also in some specialized glia (Minelli et al., 1995). GAT-1 is thought to play a key role in epileptogenesis (Zhao et al. 2003).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC6A1
• Entrez:
  • 50676(Rat)
• Uniprot:
  • P23978(Rat)