GAT-1/SLC6A1 Antibody (HL3475)

GAT-1/SLC6A1 antibody [HL3475] detects GAT-1/SLC6A1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Green: GAT-1/SLC6A1 stained by GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:250.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GAT-1/SLC6A1 antibody [HL3475] detects GAT-1/SLC6A1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: GAT-1/SLC6A1 stained by GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:250.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Unboiled human tissue extract was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

GAT-1/SLC6A1 antibody [HL3475] detects GAT-1/SLC6A1 protein by immunohistochemical analysis.Sample: Paraffin-embedded human tissues.GAT-1/SLC6A1 stained by GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 minCorresponding RNA levels (RPKM) in the tissues are based on NCBI database.

Unboiled various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Boiled and unboiled rat tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

GAT-1/SLC6A1 antibody [HL3475] detects GAT-1/SLC6A1 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse eye.Green: GAT-1/SLC6A1 stained by GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:250.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: HL3475
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

GAT-1/SLC6A1 Antibody (HL3475) Summary

• Description: Novus Biologicals Rabbit GAT-1/SLC6A1 Antibody (HL3475) (NBP3-48818) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein encompassing a sequence within the Extracellular domain of mouse GAT-1/SLC6A1. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SLC6A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for GAT-1/SLC6A1 Antibody (HL3475)

• GABA Transporter 1
• GABATHG
• GABATR
• GABATRGAT-1
• GABT1
• GAT1
• GAT-1
• GAT1sodium- and chloride-dependent GABA transporter 1
• SLC6A1
• solute carrier family 6 (neurotransmitter transporter, GABA), member 1
• Solute carrier family 6 member 1

Background

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl negative channel associated with the GABAA receptor (GABAA-R) subtype. GABA plasma membrane transporters (GATs) influence synaptic neurotransmission by high-affinity uptake and release of GABA. To date, four distinct GABA transporters have been identified: GAT-1, GAT-2, GAT-3, and BGT-1. GAT-1, the most abundant of the transporters, is found predominantly in neurons, but also in some specialized glia (Minelli et al., 1995). GAT-1 is thought to play a key role in epileptogenesis (Zhao et al. 2003).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC6A1